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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0512 [new locus tag: SA_RS02990 ]
  • pan locus tag?: SAUPAN002326000
  • symbol: ilvE
  • pan gene symbol?: ilvE
  • synonym:
  • product: branched-chain amino acid aminotransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0512 [new locus tag: SA_RS02990 ]
  • symbol: ilvE
  • product: branched-chain amino acid aminotransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 599248..600324
  • length: 1077
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTCACAAGCAGTTAAAGTTGAACGACGAGAAACATTAAAACAAAAACCAAATACATCT
    CAACTAGGTTTTGGTAAATATTTTACTGATTATATGTTGAGTTATGATTATGATGCAGAT
    AAAGGATGGCATGATTTGAAGATAGTACCTTATGGTCCTATTGAAATTTCACCTGCTGCA
    CAAGGTGTTCATTATGGTCAATCGGTATTCGAAGGATTAAAAGCATATAAAAGAGATGGG
    GAAGTTGCACTTTTCCGTCCTGAAGAAAATTTTAAGCGTCTTAATAACTCGTTAGCACGA
    TTAGAAATGCCTCAAGTAGACGAAGCAGAATTGTTAGAGGGGCTAAAACAATTAGTTGAT
    ATAGAAAGAGATTGGATTCCTGAAGGGGAAGGTCAATCATTATATATTCGTCCATTTGTT
    TTTGCAACAGAAGGGGCACTTGGCGTTGGTGCATCACATCAGTATAAATTATTAATTATT
    TTATCTCCTTCAGGTGCATATTATGGTGGTGAGACTTTAAAACCAACTAAAATCTATGTA
    GAAGATGAATATGTGCGTGCTGTTCGTGGCGGTGTAGGCTTTGCAAAAGTTGCAGGTAAC
    TATGCGGCAAGTTTATTAGCACAAACAAATGCAAATAAATTAGGTTATGACCAAGTATTA
    TGGCTTGATGGTGTTGAACAGAAATATATCGAAGAAGTTGGTAGCATGAATATTTTCTTC
    GTTGAAAATGGAAAAGTAATTACACCAGAGTTGAATGGCAGTATTTTACCTGGTATTACA
    CGTAAATCTATTATCGAATTAGCTAAAAACTTAGGATATGAAGTCGAAGAGCGCCGCGTT
    TCAATCGATGAATTATTCGAATCATATGATAAAGGTGAGTTAACAGAAGTATTTGGTAGT
    GGTACTGCAGCAGTTATTTCACCTGTGGGTACATTGAGATACGAAGATCGTGAAATCGTT
    ATTAATAATAATGAGACTGGTGAAATTACTCAAAAATTATACGACGTCTATACTGGTATT
    CAAAATGGTACTTTAGAAGATAAAAATGGTTGGAGAGTCGTTGTACCAAAATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1077

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0512 [new locus tag: SA_RS02990 ]
  • symbol: IlvE
  • description: branched-chain amino acid aminotransferase
  • length: 358
  • theoretical pI: 4.62585
  • theoretical MW: 40085.9
  • GRAVY: -0.349721

⊟Function[edit | edit source]

  • reaction:
    EC 2.6.1.42?  ExPASy
    Branched-chain-amino-acid transaminase L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate? L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate?
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family branched-chain amino acid aminotransferase (TIGR01123; EC 2.6.1.42; HMM-score: 399.5)
    and 3 more
    Metabolism Amino acid biosynthesis Pyruvate family branched-chain amino acid aminotransferase (TIGR01122; EC 2.6.1.42; HMM-score: 184.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid aminodeoxychorismate lyase (TIGR03461; EC 4.1.3.38; HMM-score: 57.1)
    Metabolism Energy metabolism Amino acids and amines D-amino-acid transaminase (TIGR01121; EC 2.6.1.21; HMM-score: 55.8)
  • TheSEED  :
    • Branched-chain amino acid aminotransferase (EC 2.6.1.42)
    Amino Acids and Derivatives Alanine, serine, and glycine Alanine biosynthesis  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
    and 6 more
    Amino Acids and Derivatives Branched-chain amino acids Branched-Chain Amino Acid Biosynthesis  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
    Amino Acids and Derivatives Branched-chain amino acids Isoleucine degradation  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
    Amino Acids and Derivatives Branched-chain amino acids Leucine Biosynthesis  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
    Amino Acids and Derivatives Branched-chain amino acids Valine degradation  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
    Carbohydrates Central carbohydrate metabolism Dehydrogenase complexes  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
    Carbohydrates Central carbohydrate metabolism Pyruvate Alanine Serine Interconversions  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
  • PFAM:
    no clan defined Aminotran_4; Amino-transferase class IV (PF01063; HMM-score: 132.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013678
    • TAT(Tat/SPI): 0.001197
    • LIPO(Sec/SPII): 0.000946
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWHDLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLEMPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLIILSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQVLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVEERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLYDVYTGIQNGTLEDKNGWRVVVPKY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

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