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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0600 [new locus tag: SACOL_RS03110 ]
  • pan locus tag?: SAUPAN002326000
  • symbol: ilvE
  • pan gene symbol?: ilvE
  • synonym:
  • product: branched-chain amino acid aminotransferase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0600 [new locus tag: SACOL_RS03110 ]
  • symbol: ilvE
  • product: branched-chain amino acid aminotransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 626612..627688
  • length: 1077
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGTCACAAGCAGTTAAAGTTGAACGACGAGAAACATTAAAACAAAAACCAAATACATCT
    CAACTAGGTTTTGGTAAATATTTTACTGATTATATGTTGAGTTATGATTATGATGCAGAT
    AAAGGATGGCATGATTTGAAGATAGTACCTTATGGTCCTATTGAAATTTCACCTGCTGCA
    CAAGGTGTTCATTATGGTCAATCGGTATTCGAAGGATTAAAAGCATATAAAAGAGATGGG
    GAAGTTGCACTTTTCCGTCCTGAAGAAAATTTTAAGCGTCTTAATAACTCGTTAGCACGA
    TTAGAAATGCCTCAAGTAGACGAAGCAGAATTGTTAGAGGGGCTAAAACAATTAGTTGAT
    ATTGAAAGAGATTGGATTCCTGAAGGGGAAGGTCAATCATTATATATTCGTCCATTTGTT
    TTTGCAACAGAAGGGGCACTTGGCGTTGGTGCATCACATCAGTATAAATTATTAATTATT
    TTATCTCCTTCAGGTGCATATTATGGTGGTGAAACTTTAAAACCAACTAAAATCTATGTA
    GAAGATGAATATGTGCGTGCTGTTCGTGGCGGTGTAGGCTTTGCAAAAGTTGCAGGTAAC
    TATGCGGCAAGTTTATTAGCACAAACTAATGCAAATAAATTAGGTTATGACCAAGTATTA
    TGGCTTGATGGTGTTGAACAGAAATATATCGAAGAAGTTGGTAGCATGAACATTTTCTTC
    GTTGAAAATGGCAAAGTAATTACACCAGAGTTGAATGGCAGTATTTTACCTGGTATTACA
    CGTAAATCTATTATCGAATTAGCTAAAAACTTAGGATATGAAGTCGAAGAGCGCCGCGTT
    TCAATCGATGAATTATTCGAATCATATGATAAAGGTGAGTTAACAGAAGTATTTGGTAGT
    GGTACTGCAGCAGTTATTTCACCTGTGGGTACATTGAGATACGAAGATCGTGAAATCGTT
    ATTAATAATAATGAGACTGGTGAAATTACTCAAAAATTATACGACGTCTATACTGGTATT
    CAAAATGGTACTTTAGAAGATAAAAATGGTTGGAGAGTCGTTGTACCAAAATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1077

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0600 [new locus tag: SACOL_RS03110 ]
  • symbol: IlvE
  • description: branched-chain amino acid aminotransferase
  • length: 358
  • theoretical pI: 4.62585
  • theoretical MW: 40085.9
  • GRAVY: -0.349721

Function[edit | edit source]

  • reaction:
    EC 2.6.1.42?  ExPASy
    Branched-chain-amino-acid transaminase L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate? L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate?
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family branched-chain amino acid aminotransferase (TIGR01123; EC 2.6.1.42; HMM-score: 399.5)
    and 3 more
    Metabolism Amino acid biosynthesis Pyruvate family branched-chain amino acid aminotransferase (TIGR01122; EC 2.6.1.42; HMM-score: 184.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid aminodeoxychorismate lyase (TIGR03461; EC 4.1.3.38; HMM-score: 57.1)
    Metabolism Energy metabolism Amino acids and amines D-amino-acid transaminase (TIGR01121; EC 2.6.1.21; HMM-score: 55.8)
  • TheSEED:  
    Amino Acids and Derivatives Alanine, serine, and glycine Alanine biosynthesis  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
    and 6 more
    Branched-chain amino acids Branched-Chain Amino Acid Biosynthesis  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
    Isoleucine degradation  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
    Leucine Biosynthesis  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
    Valine degradation  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
    Carbohydrates Central carbohydrate metabolism Dehydrogenase complexes  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
    Pyruvate Alanine Serine Interconversions  Branched-chain amino acid aminotransferase (EC 2.6.1.42)
  • PFAM:
    no clan defined Aminotran_4; Amino-transferase class IV (PF01063; HMM-score: 132.6)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013678
    • TAT(Tat/SPI): 0.001197
    • LIPO(Sec/SPII): 0.000946
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDADKGWHDLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKRDGEVALFRPEENFKRLNNSLARLEMPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLIILSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQVLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVEERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLYDVYTGIQNGTLEDKNGWRVVVPKY

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
    experimental localization: Cytoplasmic [1] [2] [3]
    quantitative data / protein copy number per cell: 7538 [4]

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise  

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: 38.95 h [5]

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

Relevant publications[edit | edit source]