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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0518 [new locus tag: SA_RS03020 ]
  • pan locus tag?: SAUPAN002333000
  • symbol: SA0518
  • pan gene symbol?: azo1
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0518 [new locus tag: SA_RS03020 ]
  • symbol: SA0518
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 604220..604786
  • length: 567
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAAAGGATTAATTATTATTGGCAGTGCACAAGTGAATTCACATACAAGTGCACTAGCA
    AGATACTTAACTGAGCATTTTAAAACACATGATATTGAAGCGGAAATATTCGATTTAGCA
    GAAAAACCGTTAAATCAATTAGATTTTTCAGGAACAACACCGTCTATTGATGAAATCAAA
    CAAAATATGAAAGATTTAAAAGAGAAAGCAATGGCGGCGGACTTTTTAATATTAGGAACG
    CCAAACTATCATGGTTCATATTCTGGAATATTGAAAAATGCATTAGATCATCTAAATATG
    GATTATTTTAAAATGAAACCTGTAGGCTTAATAGGAAATAGTGGTGGTATTGTTAGTTCA
    GAGCCATTGTCACATTTAAGAGTAATCGTCAGAAGTTTACTAGGCATTGCTGTACCAACT
    CAAATAGCAACACATGATTCTGATTTTGCTAAAAATGAAGATGGTTCATATTACTTAAAT
    GATAGTGAATTCCAATTACGAGCAAGATTATTTGTCGATCAAATTGTATCTTTTGTGAAT
    AATAGTCCATATGAACATTTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    567

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0518 [new locus tag: SA_RS03020 ]
  • symbol: SA0518
  • description: hypothetical protein
  • length: 188
  • theoretical pI: 6.07624
  • theoretical MW: 20911.6
  • GRAVY: -0.191489

⊟Function[edit | edit source]

  • reaction:
    EC 1.7.-.-?  ExPASy
  • TIGRFAM:
    FMN reductase (TIGR03566; EC 1.5.1.-; HMM-score: 54.1)
    Metabolism Central intermediary metabolism Sulfur metabolism FMN reductase (TIGR03567; EC 1.5.1.38; HMM-score: 48.9)
    and 4 more
    LLM-partnered FMN reductase, CE1759 family (TIGR04037; EC 1.-.-.-; HMM-score: 35.4)
    Cellular processes Cellular processes Detoxification arsenical resistance protein ArsH (TIGR02690; HMM-score: 30.1)
    Genetic information processing Protein synthesis Translation factors ribosome silencing factor (TIGR00090; HMM-score: 13)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides cytidine deaminase (TIGR01355; EC 3.5.4.5; HMM-score: 11.5)
  • TheSEED  :
    • Predicted flavoprotein
  • PFAM:
    Flavoprotein (CL0042) FMN_red; NADPH-dependent FMN reductase (PF03358; HMM-score: 100.7)
    and 1 more
    Flavodoxin_2; Flavodoxin-like fold (PF02525; HMM-score: 42.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FMN
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.030316
    • TAT(Tat/SPI): 0.000993
    • LIPO(Sec/SPII): 0.003323
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPLNQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDYFKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLNDSEFQLRARLFVDQIVSFVNNSPYEHLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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