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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA0522 [new locus tag: SA_RS03040 ]
- pan locus tag?: SAUPAN002338000
- symbol: SA0522
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA0522 [new locus tag: SA_RS03040 ]
- symbol: SA0522
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 616540..618012
- length: 1473
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1123327 NCBI
- RefSeq: NP_373775 NCBI
- BioCyc: see SA_RS03040
- MicrobesOnline: 102801 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1441ATGAATTACATTTTAGGAACAATTTTAGAAAGTAAAATTACAGGTGTAGAAAAAGCGCAA
ATAAATAGATTGAAGTTGTTCAAACAACACGGCATATCTTCAAAATGTGTATATGTTAAA
TGGAATCCTTATTCATACACATATGCGAAGCAACATCAGATTGAAAATGATGTATTTACA
ATGTATGACTATTTTCAAAAAGCAATCAATTATAAAAAGACAAAGCAAGTTAACTGGATA
CAGTATTGGGAAAAGTCATGTAGGTACACATTGAAATTTGTGGAAAATTCAAATGATGTC
AGAATATATGATGAAGAGCAATTTGTAATGTATGCTCATTTTTTAGATAAACAGTATCAT
CAATTAAACTATGTGAATTATTTTGATCATAAAAGAAGAAAAGTAAAACGCGAATTGTAT
GATGGAAGAGGCTTTTTAAGTTGTTCTCGAATTTTAGGTGAAGGACAACGGATTGTACTC
GAAAATTACTATACACCTAATGGGGAAATCGTCATCCAAAAATATTTCGACGATATAAAA
GGAAAAAACACGCTCACAAAGGTTATCTTAAATGAAGACCAGCAGCAACAATTTTTTGAT
ACAGAAGATGAATTAGTTCAATATTTTCTCCATCAATTATGTAAAAATAATGATCAAATC
ATATTAGATCGTCCTCATGAATTAGGAAATGTTATAGCGGGATTAAATCAAAGTATTCCA
GTTGTTGTTGTGCTCCATAGTACACATTTATCCGGTACCGGTAATGGTATAAAAAGTTTT
TATAAAACAGTATTTAATAATTTAACACGTTATAAAGCGATTGTTGTATCAACAGAACAG
CAATGCCAAGATATTTCACAATATATTGAAAATAAAATACCAGTTATCAATATTCCGGTT
GGCTACGTGGCAAATTTAAAGTATCAATTTGACATCAATCAAAAGGAGAAAAATCATATT
ATATCAATTGCTCGCCTCGTTGAAAATAAACAAATTAAACATCAAATTGAAGTGATCAAG
CAATTAGTAACAAAACATCCCAATATTCAATTGAATATTTATGGACATGGAAATGGTTTG
TCAGAATATCGACAACTTGTAGAAGATTATCATTTATCGGAACATGTTAAATTTCATGGT
TTTAAGACGCATATTAATGAAGAGATTGCTAAAGCAGAACTGATGTTATCGACAAGTAAA
ATGGAAGGTTTTGGCTTAGCAATTTTAGAGTCGCTTTCAGTAGGTACACCAGTGATCAGT
TATGATATAGATTATGGGCCATCAGAACTGATTCAAGATGGATTTAATGGCTATTTAGTA
CCTAAAGGTGACATCAATCAAATGGTTGAAAAGGTCGATCAATTACTAAATAATACTCAA
AAATTGCAACAGTTTTCGATTAATAGCATAGAATCTGCACAACAGTACAATGCAACTACT
ATCAGTACAAAGTGGCAAAATATTTTAAACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA0522 [new locus tag: SA_RS03040 ]
- symbol: SA0522
- description: hypothetical protein
- length: 490
- theoretical pI: 7.95544
- theoretical MW: 57213.6
- GRAVY: -0.479184
⊟Function[edit | edit source]
- TIGRFAM: Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 151.6)and 12 moreBiosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 104.8)sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 69.3)colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 63.5)putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 50.5)sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 38.2)D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 35.3)glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 34)PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 33.7)Protein fate Protein modification and repair accessory Sec system glycosyltransferase GtfB (TIGR02919; EC 2.4.1.-; HMM-score: 33.2)Energy metabolism Biosynthesis and degradation of polysaccharides glycogen/starch synthase, ADP-glucose type (TIGR02095; EC 2.4.1.21; HMM-score: 29.2)Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 27)Energy metabolism Biosynthesis and degradation of polysaccharides glycogen synthase, Corynebacterium family (TIGR02149; HMM-score: 25.7)
- TheSEED :
- Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52)
- PFAM: GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 131.4)and 2 moreGlyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 85.2)Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 26.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.036755
- TAT(Tat/SPI): 0.000684
- LIPO(Sec/SPII): 0.007062
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPYSYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRIYDEEQFVMYAHFLDKQYHQLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVLENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQQQQFFDTEDELVQYFLHQLCKNNDQIILDRPHELGNVIAGLNQSIPVVVVLHSTHLSGTGNGIKSFYKTVFNNLTRYKAIVVSTEQQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKHQIEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKAELMLSTSKMEGFGLAILESLSVGTPVISYDIDYGPSELIQDGFNGYLVPKGDINQMVEKVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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