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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA0523 [new locus tag: SA_RS03045 ]
- pan locus tag?: SAUPAN002340000
- symbol: SA0523
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA0523 [new locus tag: SA_RS03045 ]
- symbol: SA0523
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 618137..619627
- length: 1491
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1123328 NCBI
- RefSeq: NP_373776 NCBI
- BioCyc: see SA_RS03045
- MicrobesOnline: 102802 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1441ATGAATTATTTTGTAGGTAATAGTTTAGGTGTTAATTTAACTGGGATAGAAAAAGCAATT
ATTAATCGTTTAAATTTATTTAAAGAAATGGGAAGACCAGCGCAATGTGTATTTCTGTCA
TGGAATAGATATTTATATAGAAATGCCCAAAATTATATTACTAGTTCTGACTATATTAAT
ATGTATGATTTCTTCCAAGAAGCAACATATTTAGAACGCAATGAACCATTTGACTGGTTG
TCGTATTGGACTGATGAATGTCATTATACTTTAAAACATGTTGAAAATAGTCATGACTTT
AGAATATATGATCAAGAGCGTTTCTTAATGTATGCCCATTTTCAAGATCCTAAATATCGT
ATATTAGATTATGTGAATCATTTTGATAGTCAAAGACGAAAAGTAAAACGTGATTTTTAT
GATGTGCGTGGCTTTTTAAGTTGTAGTAGGATTCTTGTTGATAAACAACAAACATTATGT
GAATTTTTCTATAATCCAGAAGGTGACACAAAATTAGAAAAGTATTTTTCTTACAAGGAT
GGCAAACCAGAAGTACAAAAAATTATTGTTTACTATGCTAACAAACAATATTTCTTTAAT
AATGAAACGGAATTAGGCGCATTTTTCATTAAACAACTATATCAACATGGGGATTTATTT
TTCAGTGACCGTAATGTGTATACAGCACCTATTTTCAACTTAACACCTGAGTCAATCCCA
GTAGTAGCTGTGCTTCACAGTACACACATTAAAAATATTGATGCATTAGATTCATCGCCA
TTTAAAAATGTATATAAAGCGATGTTTGAAAATTTATCTCGATATCGTGCAATTATCGTT
TCTACTGAGCAGCAAAAATTAGATGTTGAAAAACGTATTAACCATACTATTCCAATTGTT
AATATTCCTGTCGGCTATAGTGAAACAATTGATACACCAGTCCAAACATTAGATCAGCGC
AGTGTTAAATTAATTTCCGTTGCTCGTTATTCACCTGAAAAACAATTACATCAACAAATT
GAATTAATTAAACGCTTAGTATCGTATGTCCCAAAAATAGAGCTTCATATGTATGGTTTT
GGTTCTGAAAGTAAAAAATTAAATGAATTGATACAAAAATATGGATTGGAAAATCATGTT
TACTTACGTGGCTTTTTAAGCAATCTAAACCAAGAATACAGCGATGCTTACTTAAGTTTA
ATAACAAGTAACATGGAAGGTTTCTCTTTAGCGCTACTTGAATCTTTAGCACATGGTGTT
CCAGTTATTAGTTATGATATTAAATATGGACCTAACGAATTGATTACGCCTGATTTCAAT
GGTTATTTAATTACTAAAAATGATGAAGATGCCCTATTTGATAAAGTAAAATATGTGATT
GATCATCCTGAAGTACAACAACGACTGTCAAAAGGTAGTTTGGCAAAAGCGCAACAATAT
AGTAAAGCGTCGTTAATTAAACAATGGGACCAATTTGTTCGTCTTATATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA0523 [new locus tag: SA_RS03045 ]
- symbol: SA0523
- description: hypothetical protein
- length: 496
- theoretical pI: 7.03865
- theoretical MW: 58441
- GRAVY: -0.423185
⊟Function[edit | edit source]
- TIGRFAM: Protein fate Protein modification and repair accessory Sec system glycosylation protein GtfA (TIGR02918; EC 2.4.1.-; HMM-score: 130.3)and 11 moreBiosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs N-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 76.8)colanic acid biosynthesis glycosyltransferase WcaL (TIGR04005; EC 2.4.-.-; HMM-score: 63.9)sugar transferase, PEP-CTERM/EpsH1 system associated (TIGR03088; HMM-score: 60.3)putative glycosyltransferase, TIGR04348 family (TIGR04348; EC 2.4.1.-; HMM-score: 28.3)PEP-CTERM/exosortase A-associated glycosyltransferase, Daro_2409 family (TIGR04063; EC 2.4.-.-; HMM-score: 26.4)Protein fate Protein modification and repair accessory Sec system glycosyltransferase GtfB (TIGR02919; EC 2.4.1.-; HMM-score: 25)D-inositol-3-phosphate glycosyltransferase (TIGR03449; EC 2.4.1.250; HMM-score: 24.9)glycosyltransferase, GG-Bacteroidales peptide system (TIGR04157; EC 2.4.1.-; HMM-score: 24.5)sucrose-phosphate synthase, putative, glycosyltransferase domain (TIGR02472; EC 2.4.1.14; HMM-score: 21.8)Unknown function Enzymes of unknown specificity glycosyltransferase, MSMEG_0565 family (TIGR04047; HMM-score: 19.7)colanic acid biosynthesis glycosyltransferase WcaC (TIGR04015; EC 2.4.-.-; HMM-score: 17.3)
- TheSEED :
- Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52)
- PFAM: GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 110.5)and 4 moreGlyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 71.7)Glyco_trans_1_2; Glycosyl transferases group 1 (PF13524; HMM-score: 25.7)NTF2 (CL0051) Ring_hydroxyl_B; Ring hydroxylating beta subunit (PF00866; HMM-score: 17.3)no clan defined ETC_C1_NDUFA5; ETC complex I subunit conserved region (PF04716; HMM-score: 14.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005522
- TAT(Tat/SPI): 0.000307
- LIPO(Sec/SPII): 0.001655
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRYLYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRIYDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLCEFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGDLFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYRAIIVSTEQQKLDVEKRINHTIPIVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEKQLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLNQEYSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITPDFNGYLITKNDEDALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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