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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0551 [new locus tag: SA_RS03185 ]
  • pan locus tag?: SAUPAN002375000
  • symbol: SA0551
  • pan gene symbol?: merA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0551 [new locus tag: SA_RS03185 ]
  • symbol: SA0551
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 644319..645767
  • length: 1449
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    TTGCTCGTAATATTGCAACTACCATGTCACATTATCAGCAAGACGAACAGAATATCATTA
    TTAATTTTTATAATGAAAAAACAATCAAAGATGTCATTGAAGACATTCAAAAGGAGGATT
    TATGTCATGAAAACATATGATTTAATTGTAATAGGATTTGGGAAAGCTGGTAAAACTTTA
    GCGAAATATGCTGCATCAACAGGTCAACACGTCGCAGTTATCGAACAATCTTCGAAAATG
    TATGGCGGCACTTGTATAAATATAGGATGTATACCTTCGAAGACACTTGTACATGATGGA
    TTAGAAGGCAAATCTTTTGAAGCAAGTTATAACCGTAAAAACGATGTTGTCAATGCGCTA
    AACAATAAAAATTACCACTTATTAGCAGACGATAACAACATTGATGTATTGGATTTTAAA
    GCGCAGTTTAAATCTAATACTGAAGTGAATTTATTAGATCAGCATGGCGATATCGTCGAT
    AGTATTACTGCACCTCATATCATTATTAATACCGGTGCTACCTCTATCATTCCTAACATA
    AAAGGCCTTGATCAAGCTAAACATGTCTTCGATTCAACAGGTTTATTAAACATTAGCTAT
    CAACCTAAGCATCTCGTTATTGTAGGTGGCGGTTATATTGCCTTAGAATTTGCTTCAATG
    TTTGCGAATTTAGGTAGTAAGGTAACAGTATTAGAACGTGGTGAATCATTTATGCCACGC
    GAAGATCAAGATGTCGTTGCACATGCTATTACTGACTTAGAAAATAAAGGCATTGCATTG
    CATACAAATGTTGAAACGACTGAATTGTCATCTGACGATCATCATACAACAGTCCGTACT
    AATGTTGGCAACTTTGAGGCTGATGCAGTACTTTTGGCTATCGGGCGCAAACCGAATACA
    GCCTTAGCTTTAGAAAATACTGATATCGAATTAGGTGATAGAGGCGAAATTAAAGTCAAT
    GCTCAACTTCAAACATCTGTGCCACATATTTATGCTGCAGGTGATGTTAAAGGCGGACTT
    CAATTTACGTATATATCTTTAGATGATTATCGAATTATCAAATCAGCGTTATATGGTAAT
    CAGTCACGTACGACTGACAATAGAGGTAGCGTGCCTTATACAGTATTTATAGATCCACCA
    TTATCACGTGTTGGATTAACTAGTAAAGAAGCTGCCGCTCAACATTATGATTACACTGAA
    CATCAACTTTTAGTAAGTGCTATACCTCGTCACAAAATTAACAATGATCCAAGAGGTTTA
    TTTAAAGTAGTCATTAATAATGAAAATAATATGATTTTAGGTGCTACATTATATGGTAAG
    CAATCTGAAGAATTAATTAATATAATTAAACTTGCGATTGATCAAAACATTCCATATACC
    GTATTACGAGATAATATTTATACACATCCTACGATGGCCGAATCATTTAATGATTTATTT
    AATTTCTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1449

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0551 [new locus tag: SA_RS03185 ]
  • symbol: SA0551
  • description: hypothetical protein
  • length: 482
  • theoretical pI: 6.99987
  • theoretical MW: 53318.4
  • GRAVY: -0.15249

⊟Function[edit | edit source]

  • TIGRFAM:
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 321)
    Cellular processes Cellular processes Detoxification mercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 305.4)
    and 41 more
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01424; EC 1.8.1.7; HMM-score: 243.9)
    mycothione reductase (TIGR03452; EC 1.8.1.15; HMM-score: 204.7)
    Metabolism Energy metabolism Electron transport glutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 200.8)
    thioredoxin and glutathione reductase (TIGR01438; EC 1.6.4.-; HMM-score: 161.2)
    trypanothione-disulfide reductase (TIGR01423; EC 1.8.1.12; HMM-score: 148.6)
    Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 122.6)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 92.7)
    Metabolism Energy metabolism Electron transport thioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 78.6)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 67.1)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 67.1)
    Metabolism Amino acid biosynthesis Glutamate family glutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 60.2)
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 52.9)
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 49.9)
    Unknown function Enzymes of unknown specificity putative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 49.2)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 40)
    Unknown function Enzymes of unknown specificity flavoprotein, HI0933 family (TIGR00275; HMM-score: 38)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 37.9)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 34)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 34)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 31.9)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 28)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 26)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis enzyme (TIGR00292; HMM-score: 25.4)
    Genetic information processing Protein fate Protein folding and stabilization xanthine dehydrogenase accessory protein XdhC (TIGR02964; HMM-score: 22.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other xanthine dehydrogenase accessory protein XdhC (TIGR02964; HMM-score: 22.4)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 19.8)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-galactopyranose mutase (TIGR00031; EC 5.4.99.9; HMM-score: 18.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 17.5)
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 17.3)
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 17.2)
    mycofactocin system FadH/OYE family oxidoreductase 1 (TIGR03996; EC 1.-.-.-; HMM-score: 15.9)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylD (TIGR03911; HMM-score: 15.2)
    Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 14.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 14.9)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 14.7)
    Metabolism Energy metabolism Amino acids and amines alanine dehydrogenase (TIGR00518; EC 1.4.1.1; HMM-score: 14.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other C-3',4' desaturase CrtD (TIGR02733; EC 1.3.99.-; HMM-score: 13.6)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, beta subunit family (TIGR01373; HMM-score: 12.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other phytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 11.2)
    lycopene cyclase family protein (TIGR01790; HMM-score: 10.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 9.5)
  • TheSEED  :
    • RclA-like protein
    Carbohydrates Central carbohydrate metabolism TCA Cycle  Putative Dihydrolipoamide dehydrogenase (EC 1.8.1.4)
    and 3 more
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Mercuric reductase  Mercuric ion reductase (EC 1.16.1.1)
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Mercuric reductase  PF00070 family, FAD-dependent NAD(P)-disulphide oxidoreductase
    Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Mercury resistance operon  Mercuric ion reductase (EC 1.16.1.1)
  • PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 204.6)
    and 21 more
    Reductase_C (CL0608) Pyr_redox_dim; Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain (PF02852; HMM-score: 81.7)
    NADP_Rossmann (CL0063) Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 81)
    Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 52.2)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 33.8)
    K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 28.6)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 27.4)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 26.2)
    XdhC_C; XdhC Rossmann domain (PF13478; HMM-score: 26)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 24.7)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 23)
    Thi4; Thi4 family (PF01946; HMM-score: 22.9)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 22.3)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 21.7)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 21.3)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 20.3)
    Lycopene_cycl; Lycopene cyclase protein (PF05834; HMM-score: 19.8)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 16.2)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 15.9)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 15.9)
    no clan defined DUF4147; Domain of unknown function (DUF4147) (PF13660; HMM-score: 13.4)
    NADP_Rossmann (CL0063) Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00786
    • TAT(Tat/SPI): 0.0008
    • LIPO(Sec/SPII): 0.001937
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLVILQLPCHIISKTNRISLLIFIMKKQSKMSLKTFKRRIYVMKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSSKMYGGTCINIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFKSNTEVNLLDQHGDIVDSITAPHIIINTGATSIIPNIKGLDQAKHVFDSTGLLNISYQPKHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAHAITDLENKGIALHTNVETTELSSDDHHTTVRTNVGNFEADAVLLAIGRKPNTALALENTDIELGDRGEIKVNAQLQTSVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVFIDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILGATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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