PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0693 [new locus tag: SA_RS03950 ]
• pan locus tag^?: SAUPAN002642000
• symbol: murB
• pan gene symbol^?: murB
• synonym:
• product: UDP-N-acetylenolpyruvoylglucosamine reductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0693 [new locus tag: SA_RS03950 ]
• symbol: murB
• product: UDP-N-acetylenolpyruvoylglucosamine reductase
• replicon: chromosome
• strand: -
• coordinates: 791508..792431
• length: 924
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123500 NCBI
• RefSeq: NP_373948 NCBI
• BioCyc: see SA_RS03950
• MicrobesOnline: 102974 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  GTGATAAATAAAGACATCTATCAAGCTTTACAACAACTTATCCCAAATGAAAAAATTAAA
  GTTGATGAACCTTTAAAACGATACACTTATACTAAAACAGGTGGTAATGCCGACTTTTAT
  ATTACCCCTACTAAAAATGAAGAAGTACAAGCAGTTGTTAAATATGCCTATCAAAATGAG
  ATTCCTGTTACATATTTAGGAAATGGCTCAAATATTATTATCCGTGAAGGTGGTATTCGC
  GGCATTGTAATTAGTTTATTATCACTAGATCATATCGATGTATCTGATGATGCGATAATA
  GCCGGTAGCGGCGCTGCAATTATTGATGTCTCACGTGTTGCTCGTGATTACGCACTTACT
  GGCCTTGAATTTGCATGTGGTATTCCAGGTTCAATTGGTGGTGCAGTGTATATGAATGCT
  GGCGCTTATGGTGGCGAAGTTAAAGATTGTATAGACTATGCGCTTTGCGTAAACGAACAA
  GGCTCGTTAATTAAACTTACAACAAAAGAATTAGAGTTAGATTATCGTAATAGCATTATT
  CAAAAAGAACACTTAGTTGTATTAGAAGCTGCATTTACTTTAGCTCCTGGTAAAATGACT
  GAAATACAAGCTAAAATGGATGATTTAACAGAACGTAGAGAATCTAAACAACCTTTAGAG
  TATCCTTCATGTGGTAGTGTATTCCAAAGACCGCCTGGTCATTTTGCAGGTAAATTGATA
  CAAGATTCTAATTTGCAAGGTCACCGTATTGGCGGCGTTGAAGTTTCAACCAAACACGCT
  GGTTTTATGGTAAATGTAGACAATGGAACTGCTACAGATTATGAAAACCTTATTCATTAT
  GTACAAAAGACCGTCAAAGAAAAATTTGGCATTGAATTAAATCGTGAAGTTCGCATTATT
  GGTGAACATCCAAAGGAATCGTAA
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⊟Protein

⊟General

• locus tag: SA0693 [new locus tag: SA_RS03950 ]
• symbol: MurB
• description: UDP-N-acetylenolpyruvoylglucosamine reductase
• length: 307
• theoretical pI: 5.24321
• theoretical MW: 33783.2
• GRAVY: -0.24202

⊟Function

• reaction:
  EC 1.3.1.98?  ExPASy
  UDP-N-acetylmuramate dehydrogenase UDP-N-acetyl-alpha-D-muramate + NADP⁺ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylenolpyruvoylglucosamine reductase (TIGR00179; EC 1.3.1.98; HMM-score: 239.1)
  and 2 more
  Metabolism Energy metabolism Other glycolate oxidase, subunit GlcD (TIGR00387; HMM-score: 17.2)
  Cellular processes Cellular processes Sporulation and germination sporulation protein YtfJ (TIGR02874; HMM-score: 12.5)
• TheSEED  :
  • UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.3.1.98)
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158)
  and 1 more
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158)
• PFAM:
  no clan defined MurB_C; UDP-N-acetylenolpyruvoylglucosamine reductase, C-terminal domain (PF02873; HMM-score: 103.7)
  FAD_PCMH (CL0077) FAD_binding_4; FAD binding domain (PF01565; HMM-score: 91.6)
  and 1 more
  no clan defined Stomoxyn; Insect antimicrobial peptide, stomoxyn (PF11585; HMM-score: 12.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: FAD
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.047164
  • TAT(Tat/SPI): 0.000865
  • LIPO(Sec/SPII): 0.003688
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926415 NCBI
• RefSeq: NP_373948 NCBI
• UniProt: P65463 UniProt

⊟Protein sequence

• MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPTKNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIDVSDDAIIAGSGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQGSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQPLEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYENLIHYVQKTVKEKFGIELNREVRIIGEHPKES

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0692 < murB

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications