PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0743 [new locus tag: SA_RS04230 ]
• pan locus tag^?: SAUPAN002791000
• symbol: SA0743
• pan gene symbol^?: vwb
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0743 [new locus tag: SA_RS04230 ]
• symbol: SA0743
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 851674..853176
• length: 1503
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123552 NCBI
• RefSeq: NP_373998 NCBI
• BioCyc: see SA_RS04230
• MicrobesOnline: 103024 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  TTGAAAAATAAATTGCTAGTTTTATCATTGGGAGCATTATGTGTATCACAAATTTGGGAA
  AGTAATCATGCGAGTGCAGTGGTTTCTGGGGAGAAGAATCCATATGTATCAAAAGCTTTA
  GAATTGAAAGATAAAAGTAATAAATCCAATTCTTACGAAAATTATAGAGATAGTTTAGAA
  AGTTTGATTTCATCATTATCTTTTGCTGATTATGAAAAATATGAAGAGCCAGAATATGAA
  AAGGCTGTAAAAAAATATCAACAAAAATTTATGGCTGAAGATGATGCATTAAAAAATTTT
  TTAAATGAAGAAAAGAAGATAAAAAATGCAGATATTAGCAGAAAATCGAATAATTTATTA
  GGTTTAACACATGAAAGATATTCTTATATTTTTGATACATTAAAGAAAAATAAACAAGAG
  TTTTTAAAAGATATTGAAGAAATACAACTGAAAAATAGTGATTTAAAGGACTTTAACAAT
  ACAGAGCAACATAATGCCGACGTAGAAATAAACAATTTAGAAAATAAAGTATTAATGGTA
  GGGTATACATTCTATAATACAAATAAGGACGAAGTTGAAGAATTATATAGTGAGTTAGAT
  TTGATTGTTGGAGAAGTTCAAGATAAGTCGGATAAAAAAAGAGCAGTAAATCAAAGGATG
  TTAAATAGAAAAAAAGAGGATTTAGAATTTATTATAGATAAATTTTTTAAAAAAATTCAA
  CAAGAACGTCCAGAGAGTATACCAGCATTAACTAGTGAAAAAAATCATAATCAGACTATG
  GCATTAAAGTTAAAAGCAGATACAGAAGCTGCTAAAAATGACGTATCAAAAAGAAGTAAA
  AGAAGTTTAAATACTCAAAATAATAAATCTACAACACAAGAAATTTCTGAAGAACAAAAA
  GCTGAATATCAAAGAAAGTCAGAGGCATTAAAAGAAAGATTTATAAACAGACAAAAATCT
  AAAAATGAGTCTGTGGTTTCACTAATCGATGACGAAGACGACAACGAAAACGACAGGCAA
  CTTGTGGTTTCTGCGCCATCAAAGAAACCAACAACACCGACTACATATACTGAAACAACG
  ACTCAGGTACCAATGCCTACAGTTGAGCGTCAAACTCAGCAACAAATCGTTTACAAAACA
  CCAAAACCATTAGCTGGATTAAATGGTGAAAGTCATGATTTCACAACAACGCATCAATCA
  CCAACAACTTCAAATCATACGCATAATAATGTTGTTGAATTTGAAGAAACGTCTGCTTTA
  CCTGGTAGAAAATCAGGATCACTGGTTGGTATAAGTCAAATTGATTCTTCTCATCTAACT
  GAACGTGAGAAGCGTGTAATCAAGCGTGAACACGTTAGAGAAGCTCAAAAGTTAGTTGAT
  AATTATAAAGATACACATAGTTATAAAGACCGATTAAATGCACAACAAAAAGTAAATACT
  TTAAGTGAAGGTCATCAAAAACGTTTTAATAAACAAATCAATAAAGTATACAATGGCAAA
  TAA
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⊟Protein

⊟General

• locus tag: SA0743 [new locus tag: SA_RS04230 ]
• symbol: SA0743
• description: hypothetical protein
• length: 500
• theoretical pI: 8.2998
• theoretical MW: 57792
• GRAVY: -1.0796

⊟Function

• TIGRFAM:
• TheSEED  :
  • Secreted von Willebrand factor-binding protein VWbp
  Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Secreted von Willebrand factor-binding protein VWbp
• PFAM:
  no clan defined Coagulase; Staphylococcus aureus coagulase (PF08764; HMM-score: 63.6)
  and 2 more
  YopE_N; YopE, N terminal (PF09020; HMM-score: 13.6)
  Oxa1 (CL0376) DUF106; Integral membrane protein DUF106 (PF01956; HMM-score: 8.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.09
  • Cellwall Score: 0.18
  • Extracellular Score: 9.73
  • Internal Helices: 0
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 26 aa
  • SP(Sec/SPI): 0.97631
  • TAT(Tat/SPI): 0.003895
  • LIPO(Sec/SPII): 0.008577
  • Cleavage Site: CS pos: 26-27. ASA-VV. Pr: 0.7788
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926465 NCBI
• RefSeq: NP_373998 NCBI
• UniProt: A0A0H3JP98 UniProt

⊟Protein sequence

• MKNKLLVLSLGALCVSQIWESNHASAVVSGEKNPYVSKALELKDKSNKSNSYENYRDSLESLISSLSFADYEKYEEPEYEKAVKKYQQKFMAEDDALKNFLNEEKKIKNADISRKSNNLLGLTHERYSYIFDTLKKNKQEFLKDIEEIQLKNSDLKDFNNTEQHNADVEINNLENKVLMVGYTFYNTNKDEVEELYSELDLIVGEVQDKSDKKRAVNQRMLNRKKEDLEFIIDKFFKKIQQERPESIPALTSEKNHNQTMALKLKADTEAAKNDVSKRSKRSLNTQNNKSTTQEISEEQKAEYQRKSEALKERFINRQKSKNESVVSLIDDEDDNENDRQLVVSAPSKKPTTPTTYTETTTQVPMPTVERQTQQQIVYKTPKPLAGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTEREKRVIKREHVREAQKLVDNYKDTHSYKDRLNAQQKVNTLSEGHQKRFNKQINKVYNGK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications