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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0744 [new locus tag: SA_RS04235 ]
  • pan locus tag?: SAUPAN002796000
  • symbol: ssp
  • pan gene symbol?: emp
  • synonym:
  • product: extracellular ECM and plasma binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0744 [new locus tag: SA_RS04235 ]
  • symbol: ssp
  • product: extracellular ECM and plasma binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 853529..854551
  • length: 1023
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAAAGAAATTATTAGTTTTAACTATGAGCACGCTATTTGCTACACAACTTATCAAT
    TCAAATCACGCTAAAGCATCAGTGACAGAGAGTGTTGACAAAAAATTTGTAGTTCCAGAA
    TCAGGAATTAATAAAATTATTCCAGCTTACGATGAATTTAAGAATTCGCCAAAAGTAAAT
    GTTAGTAATTTAACTGACAATAAAAACTTTGTAGTTTCTGAAGACAAATTGAATAAGATT
    GTAGATTCATCGGCAGCTAGTAAAATTGTAGATAAAAACTTTGCCGTACCAGAATCAAAG
    TTAGGAAACATTGTACCAGAGTACAAAGAAATCAATAATCGCGTGAATGTAGCAACAAAC
    AATCCAGCTTCACAACAAGTTGATAAGCATTTTGTTGCTAAAGGCCCAGAAGTAAATAGA
    TTTATTACGCAAAACAAAGTAAACCACCACTTCATTACTACGCAAACCCACTACAAGAAA
    GTTATTACTTCATACAAATCAACACATGTACATAAACATGTAAATCATGCAAAGGATTCT
    ATTAATAAACACTTTATTGTTAAACCATCAGAATCGCCTAGATATACACATCCATCTCAA
    TCTTTAATTATCAAGCATCATTTTGCAGTTCCTGGATATCACGCGCATAAATTTGTTACA
    CCAGGGCATGCTAGCATTAAAATTAATCACTTTTGTGTTGTGCCACAAATAAATAGTTTC
    AAGGTAATTCCACCATATGGTCACAATTCACATCGTATGCATGTACCAAGTTTCCAAAAT
    AACACAACAGCAACACATCAAAATGCTAAAGTAAATAAAGCATATGACTATAAATACTTC
    TATTCTTATAAAGTAGTTAAAGGTGTGAAGAAATATTTCTCATTTTCACAATCAAATGGT
    TATAAAATTGGGAAACCATCATTAAATATCAAAAATGTAAATTATCAATATGCTGTTCCA
    AGTTATAGCCCTACACACTACGTTCCTGAATTTAAGGGTAGCTTACCAGCACCACGAGTA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1023

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0744 [new locus tag: SA_RS04235 ]
  • symbol: Ssp
  • description: extracellular ECM and plasma binding protein
  • length: 340
  • theoretical pI: 10.4459
  • theoretical MW: 38494.6
  • GRAVY: -0.547353

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Extracellular ECM and plasma binding protein Emp
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Extracellular ECM and plasma binding protein Emp
  • PFAM:

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 9.94
    • Extracellular Score: 0.06
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: NHAKASVT
  • SignalP: Signal peptide SP(Sec/SPI) length 26 aa
    • SP(Sec/SPI): 0.995787
    • TAT(Tat/SPI): 0.00051
    • LIPO(Sec/SPII): 0.002611
    • Cleavage Site: CS pos: 26-27. AKA-SV. Pr: 0.9446
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKKLLVLTMSTLFATQLINSNHAKASVTESVDKKFVVPESGINKIIPAYDEFKNSPKVNVSNLTDNKNFVVSEDKLNKIVDSSAASKIVDKNFAVPESKLGNIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKKVITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKFVTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYDYKYFYSYKVVKGVKKYFSFSQSNGYKIGKPSLNIKNVNYQYAVPSYSPTHYVPEFKGSLPAPRV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

M Hussain, K Becker, C von Eiff, J Schrenzel, G Peters, M Herrmann
Identification and characterization of a novel 38.5-kilodalton cell surface protein of Staphylococcus aureus with extended-spectrum binding activity for extracellular matrix and plasma proteins.
J Bacteriol: 2001, 183(23);6778-86
[PubMed:11698365] [WorldCat.org] [DOI] (P p)

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