PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0804 [new locus tag: SA_RS04585 ]
• pan locus tag^?: SAUPAN003043000
• symbol: SA0804
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0804 [new locus tag: SA_RS04585 ]
• symbol: SA0804
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 909198..910514
• length: 1317
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123619 NCBI
• RefSeq: NP_374065 NCBI
• BioCyc: see SA_RS04585
• MicrobesOnline: 103091 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGATAAATGCAGTAGTAATAGCAGTAATATTAATGATTGTGCTATGTTTATGTCGATTA
  AACGTAGTTATAAGCTTATTTATCAGTGCGCTAGTTGGTGGCTTAATTTCAGGCATGAGC
  ATTGAAAAAGTTATAAATGTATTTGGGAAAAATATAGTCGATGGTGCTGAGGTAGCATTA
  AGCTATGCATTATTAGGTGGATTTGCAGCATTAATTTCATACAGTGGTATCACAGACTAT
  TTAGTAGGAAAAATTATAAATGCAATTCACGCTGAAAATAGTCGATGGTCAAGAGTTAAA
  GTAAAAGTGACAATAATCATTGCATTATTAGCTATGAGTATCATGAGTCAAAACTTAATT
  CCTGTACATATTGCATTCATTCCAATTGTCATCCCACCATTGTTAAGTCTGTTTAATGAC
  TTAAAAATAGATAGACGTTTAATCGGTTTGATTATCGGTTTTGGTTTATGTTTCCCGTAT
  GTGTTATTACCATATGGATTCGGTCAAATTTTCCAGCAAATTATTCAAAGTGGCTTTGCA
  AAGGCGAATCACCCAATTGAGTTTAATATGATTTGGAAAGCAATGCTTATTCCTTCAATG
  GGGTATATTGTTGGCTTACTTATCGGTTTATATGTATATCGTAAACCACGTGAATATGAA
  ACACGTAAAATTTCAGATAGTGACAATGTTACAGAGTTAAAACCATATATCTTAATAGTA
  ACAATTGTGGCAATACTAGCTACATTTTTAGTACAAACATTTACAGATTCAATGATTTTT
  GGTGCACTGGCAGGGGTACTCGTATTCTTTATTTCACGTGCATATAATTGGTATGAATTA
  GATGCTAAGTTTGTTGAAGGTATTAAAATTATGGCTTATATTGGTGTAGTTATTTTAACA
  GCAAATGGGTTTGCTGGTGTAATGAATGCTACTGGTGATATAGATGAATTAGTTAAAACT
  TTAACAAGTATTACTGGTGATAATAAATTATTTAGCATTATCATGATGTATGTGATAGGT
  TTAATTGTCACTTTAGGTATTGGATCATCATTTGCAACAATTCCTATTATCGCATCATTA
  TTCATTCCTTTTGGAGCGTCAATTGGACTAGATACAATGGCATTAATCGCATTGATTGGA
  ACAGCGAGTGCATTAGGTGACTCAGGTTCACCTGCAAGTGATTCAACATTAGGACCAACT
  GCGGGATTAAATGTTGATGGCCAACATGATCATATACGCGATACATGTGTACCAAACTTC
  TTGTTTTATAATATTCCTTTAATGATTTTCGGTACTATTGCTGCTATGGTACTATAA
  60
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  1317
  

⊟Protein

⊟General

• locus tag: SA0804 [new locus tag: SA_RS04585 ]
• symbol: SA0804
• description: hypothetical protein
• length: 438
• theoretical pI: 6.6689
• theoretical MW: 47279.2
• GRAVY: 0.940868

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids TRAP transporter, DctM subunit (TIGR00786; HMM-score: 23.7)
  and 1 more
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids transporter, gluconate:H+ symporter (GntP) family (TIGR00791; HMM-score: 11.4)
• TheSEED  :
  • Histidine permease YuiF
• PFAM:
  IT (CL0182) Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 223.9)
  and 6 more
  Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 110.5)
  DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 39.8)
  DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 22.5)
  no clan defined DUF969; Protein of unknown function (DUF969) (PF06149; HMM-score: 16.1)
  AzlD; Branched-chain amino acid transport protein (AzlD) (PF05437; HMM-score: 13)
  IT (CL0182) GntP_permease; GntP family permease (PF02447; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 11
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.05255
  • TAT(Tat/SPI): 0.000716
  • LIPO(Sec/SPII): 0.068386
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 15926532 NCBI
• RefSeq: NP_374065 NCBI
• UniProt: A0A0H3JM39 UniProt

⊟Protein sequence

• MINAVVIAVILMIVLCLCRLNVVISLFISALVGGLISGMSIEKVINVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVKVTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPYVLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPREYETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYNWYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSIIMMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPASDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0804 > SA0805

⊟Regulation

• regulator: HisR* (repression) regulon

  HisR*

  (TF)

  important in Histidine biosynthesis; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications