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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0946 [new locus tag: SACOL_RS04850 ]
  • pan locus tag?: SAUPAN003043000
  • symbol: SACOL0946
  • pan gene symbol?: —
  • synonym:
  • product: Na+/H+ antiporter family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0946 [new locus tag: SACOL_RS04850 ]
  • symbol: SACOL0946
  • product: Na+/H+ antiporter family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 948636..949952
  • length: 1317
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATAAATGCAGTAGTAATAGCAGTAATTTTAATGATTATGCTATGTTTATGTCGATTA
    AACGTAGTTATAAGCTTATTTATCAGTGCGCTAGTTGGTGGCTTAATTTCAGGCATGAGC
    ATTGAAAAAGTTATAAATGTATTTGGGAAAAATATAGTCGATGGTGCTGAGGTAGCATTA
    AGCTATGCTTTATTAGGTGGATTTGCAGCATTAATTTCATACAGTGGTATCACAGACTAT
    TTAGTAGGAAAAATTATAAATGCAATTCACGCTGAAAATAGTCGATGGTCAAGAGTTAAA
    GTCAAAGTGACAATAATCATTGCATTATTAGCTATGAGTATCATGAGTCAAAACTTAATT
    CCTGTACATATTGCATTCATTCCAATTGTCATCCCACCATTGTTAAGTCTGTTTAATGAC
    TTAAAAATAGATAGACGTTTAATCGGTTTGATTATCGGTTTTGGTTTATGTTTCCCGTAT
    GTGTTATTACCATATGGATTCGGTCAAATTTTCCAGCAAATTATTCAAAGTGGCTTTGCA
    AAGGCAAATCACCCAATTGAGTTTAATATGATTTGGAAAGCAATGCTTATTCCTTCAATG
    GGGTATATTGTTGGCTTACTTATCGGTTTATATGTATATCGTAAACCACGTGAATATGAA
    ACACGTAAAATTTCAGATAGTGACAATGTTACAGAGTTAAAACCATATATCTTAATAGTA
    ACAATTGTAGCAATACTAGCTACATTTTTAGTACAAACATTTACAGATTCAATGATTTTT
    GGTGCACTGGCAGGGGTACTCGTATTCTTTATTTCACGTGCATATAATTGGTATGAATTA
    GATGCTAAGTTTGTTGAAGGTATTAAAATTATGGCTTATATTGGTGTAGTTATTTTAACA
    GCAAATGGATTTGCTGGTGTAATGAATGCTACTGGTGATATAGATGAATTAGTTAAAACT
    TTAACAAGTATTACTGGTGATAATAAATTATTTAGCATTATCATGATGTATGTGATAGGT
    TTAATTGTCACTTTAGGTATTGGATCATCATTTGCAACAATTCCTATTATCGCATCATTA
    TTCATTCCTTTTGGAGCGTCAATTGGACTAGATACAATGGCATTAATCGCATTGATTGGA
    ACAGCGAGTGCATTAGGTGACTCAGGTTCGCCTGCAAGTGATTCAACATTAGGACCAACT
    GCGGGATTAAATGTTGATGGCCAACATGATCATATACGTGATACATGTGTACCAAACTTC
    TTGTTTTATAATATTCCTTTAATGATTTTCGGTACTATTGCTGCTATGGTACTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1317

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0946 [new locus tag: SACOL_RS04850 ]
  • symbol: SACOL0946
  • description: Na+/H+ antiporter family protein
  • length: 438
  • theoretical pI: 6.6689
  • theoretical MW: 47311.2
  • GRAVY: 0.935616

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids TRAP transporter, DctM subunit (TIGR00786; HMM-score: 23.6)
    and 2 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids transporter, gluconate:H+ symporter (GntP) family (TIGR00791; HMM-score: 11.8)
    Metabolism Transport and binding proteins Unknown substrate transport protein (TIGR00833; HMM-score: 2.6)
  • TheSEED  :
    • Histidine permease YuiF
  • PFAM:
    IT (CL0182) Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 223.9)
    and 6 more
    Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 109.4)
    DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 39.9)
    DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 22.4)
    no clan defined AzlD; Branched-chain amino acid transport protein (AzlD) (PF05437; HMM-score: 13)
    IT (CL0182) GntP_permease; GntP family permease (PF02447; HMM-score: 12.5)
    no clan defined DUF969; Protein of unknown function (DUF969) (PF06149; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.053737
    • TAT(Tat/SPI): 0.000756
    • LIPO(Sec/SPII): 0.07889
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MINAVVIAVILMIMLCLCRLNVVISLFISALVGGLISGMSIEKVINVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVKVTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPYVLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPREYETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYNWYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSIIMMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPASDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: HisR* (repression) regulon
    HisR*(TF)important in Histidine biosynthesis; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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