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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0947 [new locus tag: SACOL_RS04855 ]
  • pan locus tag?: SAUPAN003044000
  • symbol: SACOL0947
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0947 [new locus tag: SACOL_RS04855 ]
  • symbol: SACOL0947
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 949971..950345
  • length: 375
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    ATGACTCATTTATTAGAGACATTTGAGATGTCAATAGATCACCAGGAAGATGGTTTAGTT
    GTTATTTCTATGCCTGTTACTGATAAAGTAAAACAACCATTTGGATATTTACATGGTGGG
    GCTTCGATTGCTTTAGGTGAAACAGCATGTTCATTAGGATCTGCTAATTTAATTGATACA
    ACCAAATTTATTCCATTAGGTTTAGAGATGAATGCTAACCATATTCATTCTGCTAAAGAT
    GGTCGTGTTACTGCGACAGCTGAAATTATTCATCGAGGTAAGTCGACACATGTATGGGAT
    ATAAAAATTAAGAATGACAAAGAACAATTAATTACAGTTATGCGTGGTACAGTTGCTATT
    AAACCTTTAAAATAA
    60
    120
    180
    240
    300
    360
    375

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0947 [new locus tag: SACOL_RS04855 ]
  • symbol: SACOL0947
  • description: hypothetical protein
  • length: 124
  • theoretical pI: 7.23489
  • theoretical MW: 13544.6
  • GRAVY: -0.0669355

⊟Function[edit | edit source]

  • TIGRFAM:
    uncharacterized domain 1 (TIGR00369; HMM-score: 99.9)
    and 1 more
    phenylacetic acid degradation protein PaaD (TIGR02286; HMM-score: 38.9)
  • TheSEED  :
    • 1,4-dihydroxy-2-naphthoyl-CoA hydrolase (EC 3.1.2.28) in menaquinone biosynthesis
  • PFAM:
    HotDog (CL0050) 4HBT; Thioesterase superfamily (PF03061; HMM-score: 53.7)
    and 3 more
    DUF4442; Domain of unknown function (DUF4442) (PF14539; HMM-score: 21.5)
    4HBT_3; Thioesterase-like superfamily (PF13622; HMM-score: 17.9)
    GlnB-like (CL0089) CutA1; CutA1 divalent ion tolerance protein (PF03091; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007452
    • TAT(Tat/SPI): 0.002648
    • LIPO(Sec/SPII): 0.000704
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIALGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHRGKSTHVWDIKIKNDKEQLITVMRGTVAIKPLK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: HisR* (repression) regulon
    HisR*(TF)important in Histidine biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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