From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0900 [new locus tag: SA_RS05105 ]
  • pan locus tag?: SAUPAN003250000
  • symbol: sspB
  • pan gene symbol?: sspB
  • synonym:
  • product: cysteine protease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0900 [new locus tag: SA_RS05105 ]
  • symbol: sspB
  • product: cysteine protease
  • replicon: chromosome
  • strand: -
  • coordinates: 1020384..1021565
  • length: 1182
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATAGTTCATATAAATCTAGAGTATTCAATATTATAAGCATCATAATGGTTTCAATG
    CTTATTTTATCACTAGGCGCATTTGCTAATAACAATAAAGCCAAAGCCGATTCACACTCT
    AAACAGCTAGAAATTAATGTTAAGAGTGACAAAGTACCTCAAAAAGTAAAAGATCTAGCA
    CAACAACAATTTGCTGGTTATGCTAAAGCATTAGATAAACAAAGTAATGCAAAAACTGGT
    AAATATGAACTTGGCGAAGCTTTTAAAATTTATAAATTTAATGGTGAAGAAGATAATAGT
    TACTATTATCCAGTTATAAAAGACGGTAAAATTGTTTATACTTTAACACTTAGCCCTAAA
    AATAAAGATGATTTAAACAAATCAAAAGAAGACATGAATTACAGTGTTAAAATTTCAAAC
    TTCATCGCTAAAGATTTAGACCAAATTAAAGATAAAAATTCAAATATCACTGTTCTTACT
    GATGAAAAAGGGTTTTATTTTGAAGAAGATGGCAAAGTTAGATTAGTAAAAGCTACGCCA
    CTACCTGGTAATGTAAAAGAAAAAGAAAGTGCTAAAACAGTTTCAGCAAAATTGAAACAA
    GAGTTAAAAAATACAGTAACACCTACTAAAGTTGAAGAAAACGAAGCGATACAAGAAGAT
    CAAGTTCAATATGAAAATACATTAAAAAACTTCAAAATTAGAGAACAACAATTCGATAAC
    TCATGGTGTGCAGGATTTAGTATGGCAGCATTATTAAATGCAACTAAAAATACAGACACT
    TATAATGCACATGATATTATGCGTACATTATACCCTGAAGTAAGTGAGCAAGACCTTCCT
    AATTGCTCAACATTCCCTAATCAAATGATTGAATACGGTAAATCACAAGGCAGAGATATT
    CATTATCAAGAAGGCGTACCATCATATGAACAAGTTGATCAACTTACAAAAGATAATGTA
    GGAATTATGATTCTTGCACAAAGTGTATCTCAAAACCCTAATGATCCACATTTAGGACAT
    GCCCTAGCAGTTGTTGGTAATGCTAAAATTAATGACCAAGAAAAACTTATTTACTGGAAT
    CCTTGGGATACAGAATTATCAATCCAAGATGCAGATTCAAGCCTATTACATTTATCATTC
    AATCGTGATTATAACTGGTATGGTTCAATGATAGGTTACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1182

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0900 [new locus tag: SA_RS05105 ]
  • symbol: SspB
  • description: cysteine protease
  • length: 393
  • theoretical pI: 5.75826
  • theoretical MW: 44594.8
  • GRAVY: -0.671756

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.22.-?  ExPASy
  • TIGRFAM:
  • TheSEED  :
    • hypothetical protein
  • PFAM:
    Peptidase_CA (CL0125) Peptidase_C47; Staphopain peptidase C47 (PF05543; HMM-score: 315.5)
    Cystatin (CL0121) Staphopain_pro; Staphopain proregion (PF14731; HMM-score: 269.1)
    and 1 more
    no clan defined NusG_II; NusG domain II (PF07009; HMM-score: 7.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helix: 1
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: NKAKADSH
  • SignalP: Signal peptide SP(Sec/SPI) length 36 aa
    • SP(Sec/SPI): 0.961517
    • TAT(Tat/SPI): 0.009676
    • LIPO(Sec/SPII): 0.01917
    • Cleavage Site: CS pos: 36-37. AKA-DS. Pr: 0.7838
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNSSYKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLEINVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYYYPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLTDEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQEDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSEQDLPNCSTFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPNDPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIGY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA0900&oldid=80676"