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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0963 [new locus tag: SA_RS05455 ]
  • pan locus tag?: SAUPAN003341000
  • symbol: pycA
  • pan gene symbol?: pycA
  • synonym:
  • product: pyruvate carboxylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0963 [new locus tag: SA_RS05455 ]
  • symbol: pycA
  • product: pyruvate carboxylase
  • replicon: chromosome
  • strand: +
  • coordinates: 1091242..1094694
  • length: 3453
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    TTGAAACAAATAAAAAAGTTACTTGTTGCTAACCGTGGAGAAATTGCAATTCGTATATTC
    AGAGCGGCGGCAGAATTAGACATCAGCACAGTTGCAATTTATTCGAATGAAGACAAAAGT
    TCATTACATAGATATAAAGCAGATGAATCCTATTTAGTTGGAAGTGATTTAGGTCCTGCT
    GAAAGTTATTTAAATATTGAGCGTATCATTGATGTAGCAAAACAAGCGAATGTGGATGCG
    ATTCATCCTGGCTATGGATTTTTAAGTGAAAATGAACAATTTGCGCGTCGTTGTGCAGAA
    GAAGGAATTAAATTTATTGGTCCTCATTTAGAACATTTAGATATGTTTGGAGATAAAGTT
    AAAGCTCGTACAACGGCTATCAAGGCAGATTTACCAGTTATTCCTGGTACAGACGGTCCA
    ATTAAATCATATGAATTAGCAAAAGAATTTGCAGAAGAAGCTGGTTTCCCGCTAATGATT
    AAAGCCACAAGTGGTGGCGGCGGTAAAGGTATGAGAATCGTTCGTGAAGAAAGTGAATTA
    GAAGATGCTTTCCATAGAGCAAAATCAGAAGCTGAAAAATCATTTGGTAATAGTGAAGTT
    TACATAGAAAGATACATTGATAATCCAAAGCATATTGAAGTACAAGTCATAGGTGACGAA
    CATGGAAATATCGTACACTTATTTGAACGTGATTGTTCAGTACAACGTCGTCATCAAAAA
    GTTGTAGAAGTTGCACCATCAGTTGGATTATCACCAACATTACGTCAACGTATTTGTGAT
    GCTGCAATTCAATTGATGGAAAATATTAAATATGTCAATGCAGGTACTGTTGAATTTCTA
    GTATCTGGTGACGAATTCTTCTTTATAGAAGTTAACCCTCGTGTACAAGTAGAGCATACA
    ATTACAGAGATGGTAACAGGAATTGATATTGTTAAGACACAAATTTTAGTTGCAGCAGGT
    GCCGATTTATTTGGTGAAGAGATTAATATGCCGCAACAAAAAGATATTACAACATTAGGC
    TATGCCATCCAATGTCGTATTACAACAGAAGATCCGTTAAATGATTTCATGCCGGATACT
    GGAACAATCATTGCTTATCGTTCAAGCGGTGGCTTTGGTGTACGTCTTGATGCTGGAGAT
    GGTTTCCAAGGTGCTGAGATATCACCTTATTATGATTCATTACTCGTAAAATTATCTACA
    CATGCGATATCATTTAAACAAGCAGAAGAAAAAATGGTACGTTCATTACGAGAAATGCGT
    ATTCGTGGTGTTAAAACTAATATTCCATTCTTAATTAATGTAATGAAGAATAAAAAGTTC
    ACAAGTGGTGATTACACAACTAAATTTATTGAAGAAACACCAGAACTTTTCGACATTCAG
    CCGTCTCTAGATAGAGGTACTAAAACATTAGAATATATAGGTAATGTAACAATTAATGGT
    TTCCCAAATGTTGAGAAACGTCCGAAACCAGACTATGAATTAGCATCAATTCCAACTGTA
    TCTTCAAGTAAAATCGCTTCATTTAGTGGTACGAAACAATTGCTTGATGAAGTAGGTCCA
    AAAGGTGTAGCTGAATGGGTTAAAAAGCAGGATGATGTCTTACTAACAGATACAACCTTT
    AGAGATGCACACCAATCATTATTAGCTACACGAGTTAGAACTAAGGATATGATTAATATC
    GCATCCAAAACAGCGGACGTATTTAAAGATGGTTTCTCACTAGAAATGTGGGGCGGTGCT
    ACATTTGATGTGGCATATAATTTCTTGAAGGAAAACCCATGGGAACGACTTGAACGTCTA
    CGTAAAGCTATCCCAAATGTATTATTCCAAATGTTGTTACGTGCTTCAAACGCAGTTGGT
    TATAAAAACTATCCTGATAATGTTATTCATAAATTCGTACAAGAAAGTGCTAAAGCAGGC
    ATAGATGTCTTTAGAATTTTCGATTCATTAAACTGGGTAGATCAAATGAAAGTTGCCAAT
    GAAGCAGTACAAGAAGCGGGCAAAATCTCTGAAGGTACTATTTGTTATACAGGTGACATT
    TTAAATCCTGAGCGATCAAACATTTATACTTTAGAGTATTATGTCAAACTAGCTAAAGAG
    TTAGAACGTGAAGGTTTCCATATTTTAGCGATTAAAGATATGGCAGGCTTATTAAAACCT
    AAAGCCGCTTACGAATTGATTGGTGAGTTAAAATCAGCTGTAGATTTACCAATTCATCTT
    CACACTCATGATACAAGTGGTAATGGTTTATTAACATACAAACAAGCAATAGATGCTGGG
    GTCGATATCATTGATACTGCTGTTGCTTCAATGAGTGGTTTAACAAGTCAGCCAAGCGCC
    AATTCGTTATATTATGCATTAAATGGCTTCCCACGCCACCTTAGAACTGATATTGAAGGT
    ATGGAGTCACTTAGTCATTATTGGTCAACTGTACGTACTTATTATTCAGACTTTGAAAGT
    GATATCAAATCACCGAATACTGAAATTTATCAACATGAAATGCCTGGTGGACAGTATTCG
    AATTTAAGTCAACAAGCTAAAAGTTTAGGTTTAGGCGAAAGATTTGATGAAGTCAAAGAT
    ATGTATCGCAGAGTGAATTTCTTATTTGGTGATATCGTAAAAGTAACTCCATCGTCTAAA
    GTAGTTGGTGATATGGCACTTTATATGGTACAAAATGATCTTGATGAACAATCCGTGATT
    ACAGATGGCTATAAATTAGATTTCCCAGAATCAGTAGTGTCGTTCTTCAAAGGCGAAATA
    GGACAACCTGTAAATGGTTTTAATAAAGATTTACAAGCGGTTATTTTAAAAGGCCAAGAA
    GCACTAACAGCTCGTCCAGGTGAATATCTAGAGCCAGTTGATTTTGAAAAAGTCCGTGAG
    TTGCTTGAAGAAGAGCAACAAGGTCCTGTTACGGAGCAAGATATTATTAGTTATGTATTA
    TATCCAAAAGTATATGAACAATATATTCAAACTAGAAATCAATACGGAAACTTATCGTTA
    CTTGATACGCCTACATTCTTCTTTGGAATGCGTAATGGTGAAACAGTAGAAATCGAAATC
    GATAAGGGTAAACGATTAATTATTAAACTAGAAACGATTAGTGAACCAGATGAAAATGGT
    AATAGAACGATTTACTATGCGATGAATGGTCAAGCGAGACGTATTTACATTAAAGATGAA
    AATGTGCATACAAATGCGAACGTTAAGCCAAAAGCAGATAAGAGTAATCCAAGTCATATC
    GGTGCTCAAATGCCAGGTTCAGTAACTGAAGTCAAGGTTAGTGTTGGTGAAACTGTGAAA
    GCTAATCAGCCGTTGCTAATTACTGAAGCTATGAAAATGGAAACAACAATTCAAGCACCA
    TTTGACGGTGTGATTAAACAAGTAACTGTAAATAATGGTGACACAATAGCGACAGGCGAT
    TTATTAATCGAAATTGAAAAAGCAACTGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
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    660
    720
    780
    840
    900
    960
    1020
    1080
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    1320
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    1560
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    1680
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    1860
    1920
    1980
    2040
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    2160
    2220
    2280
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    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3453

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0963 [new locus tag: SA_RS05455 ]
  • symbol: PycA
  • description: pyruvate carboxylase
  • length: 1150
  • theoretical pI: 4.9295
  • theoretical MW: 128557
  • GRAVY: -0.326609

⊟Function[edit | edit source]

  • reaction:
    EC 6.4.1.1?  ExPASy
    Pyruvate carboxylase ATP + pyruvate + HCO3(-) = ADP + phosphate + oxaloacetate
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis pyruvate carboxylase (TIGR01235; EC 6.4.1.1; HMM-score: 1797.8)
    and 28 more
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxylase subunit (TIGR00514; EC 6.3.4.14; HMM-score: 547.7)
    Metabolism Central intermediary metabolism Nitrogen metabolism urea carboxylase (TIGR02712; EC 6.3.4.6; HMM-score: 532.3)
    Metabolism Transport and binding proteins Cations and iron carrying compounds oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 487.6)
    Metabolism Energy metabolism Other oxaloacetate decarboxylase alpha subunit (TIGR01108; EC 4.1.1.3; HMM-score: 487.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, large subunit (TIGR01369; EC 6.3.5.5; HMM-score: 67.9)
    Metabolism Energy metabolism Pyruvate dehydrogenase dihydrolipoyllysine-residue acetyltransferase (TIGR01348; EC 2.3.1.12; HMM-score: 43.4)
    alpha-L-glutamate ligase, RimK family (TIGR00768; EC 6.3.2.-; HMM-score: 42.3)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan D-alanine--D-alanine ligase (TIGR01205; EC 6.3.2.4; HMM-score: 41.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylglycinamide formyltransferase 2 (TIGR01142; EC 2.1.2.-; HMM-score: 38.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylaminoimidazole carboxylase, ATPase subunit (TIGR01161; EC 4.1.1.21; HMM-score: 38)
    Metabolism Transport and binding proteins Unknown substrate efflux transporter, RND family, MFP subunit (TIGR01730; HMM-score: 34.3)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis acetyl-CoA carboxylase, biotin carboxyl carrier protein (TIGR00531; HMM-score: 34)
    homocitrate synthase (TIGR02146; EC 2.3.3.14; HMM-score: 32.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 32)
    Metabolism Central intermediary metabolism Nitrogen fixation homocitrate synthase (TIGR02660; EC 2.3.3.14; HMM-score: 32)
    lysine biosynthesis enzyme LysX (TIGR02144; HMM-score: 28.9)
    isopropylmalate/citramalate/homocitrate synthases (TIGR02090; HMM-score: 26.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylamine--glycine ligase (TIGR00877; EC 6.3.4.13; HMM-score: 25.6)
    Metabolism Energy metabolism TCA cycle dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex (TIGR01347; EC 2.3.1.61; HMM-score: 23.7)
    Metabolism Energy metabolism Other 4-hydroxy-2-oxovalerate aldolase (TIGR03217; EC 4.1.3.39; HMM-score: 23.7)
    Cellular processes Cellular processes Biosynthesis of natural products cyanophycin synthetase (TIGR02068; EC 6.3.2.29,6.3.2.30; HMM-score: 23.2)
    2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase (TIGR02927; EC 2.3.1.61; HMM-score: 22.1)
    Metabolism Transport and binding proteins Other efflux pump membrane protein (TIGR00998; HMM-score: 18.9)
    Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 15.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system secretion protein (TIGR03794; HMM-score: 15.9)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis protein PylC (TIGR03909; HMM-score: 14.7)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 13.9)
    Metabolism Transport and binding proteins Other membrane fusion protein cluster 2 protein (TIGR00999; HMM-score: 12.7)
  • TheSEED  :
    • Pyruvate carboxylase (EC 6.4.1.1)
    Carbohydrates Central carbohydrate metabolism Pyruvate metabolism I: anaplerotic reactions, PEP  Pyruvate carboxylase (EC 6.4.1.1)
  • PFAM:
    ATP-grasp (CL0179) CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain (PF02786; HMM-score: 259.2)
    Post-HMGL (CL0597) PYC_OADA; Conserved carboxylase domain (PF02436; HMM-score: 247.1)
    and 17 more
    PreATP-grasp (CL0483) Biotin_carb_N; Biotin carboxylase, N-terminal domain (PF00289; HMM-score: 145.6)
    Hybrid (CL0105) Biotin_carb_C; Biotin carboxylase C-terminal domain (PF02785; HMM-score: 111.8)
    TIM_barrel (CL0036) HMGL-like; HMGL-like (PF00682; HMM-score: 94.3)
    Hybrid (CL0105) Biotin_lipoyl; Biotin-requiring enzyme (PF00364; HMM-score: 68.7)
    ATP-grasp (CL0179) ATPgrasp_Ter; ATP-grasp in the biosynthetic pathway with Ter operon (PF15632; HMM-score: 48.3)
    ATP-grasp; ATP-grasp domain (PF02222; HMM-score: 42.9)
    Dala_Dala_lig_C; D-ala D-ala ligase C-terminus (PF07478; HMM-score: 38)
    Hybrid (CL0105) Biotin_lipoyl_2; Biotin-lipoyl like (PF13533; HMM-score: 37.3)
    ATP-grasp (CL0179) ATP-grasp_3; ATP-grasp domain (PF02655; HMM-score: 32.2)
    Hybrid (CL0105) HlyD_D23; Barrel-sandwich domain of CusB or HlyD membrane-fusion (PF16576; HMM-score: 28)
    HlyD_3; HlyD family secretion protein (PF13437; HMM-score: 26.1)
    ATP-grasp (CL0179) RimK; RimK-like ATP-grasp domain (PF08443; HMM-score: 23.9)
    ATP-grasp_4; ATP-grasp domain (PF13535; HMM-score: 15.8)
    GARS_A; Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (PF01071; HMM-score: 14.1)
    Hybrid (CL0105) RnfC_N; RnfC Barrel sandwich hybrid domain (PF13375; HMM-score: 13.9)
    GCV_H; Glycine cleavage H-protein (PF01597; HMM-score: 12.8)
    POTRA (CL0191) YqfD; Putative stage IV sporulation protein YqfD (PF06898; HMM-score: 10.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: biotin
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008855
    • TAT(Tat/SPI): 0.000494
    • LIPO(Sec/SPII): 0.001059
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHRYKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEGIKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMIKATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDNPKHIEVQVIGDEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSPTLRQRICDAAIQLMENIKYVNAGTVEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQKDITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYDSLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFIEETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASFSGTKQLLDEVGPKGVAEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTADVFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNYPDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILNPERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHLHTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDIEGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFDEVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVSFFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTEQDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKLETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSVTEVKVSVGETVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIEKATD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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