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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0976 [new locus tag: SA_RS05535 ]
  • pan locus tag?: SAUPAN003358000
  • symbol: isdB
  • pan gene symbol?: isdB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0976 [new locus tag: SA_RS05535 ]
  • symbol: isdB
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1104658..1106595
  • length: 1938
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAACAAACAGCAAAAAGAATTTAAATCATTTTATTCAATTAGAAAGTCATCACTAGGC
    GTTGCATCTGTAGCGATTAGTACACTTTTATTATTAATGTCAAATGGCGAAGCACAAGCA
    GCAGCTGAAGAAACAGGTGGTACAAATACAGAAGCACAACCAAAAACTGAAGCAGTTGCA
    AGTCCAACAACAACATCTGAAAAAGCTCCAGAAACTAAACCAGTAGCTAATGCTGTCTCA
    GTATCTAATAAAGAAGTTGAGGCCCCTACTTCTGAAACAAAAGAAGCTAAAGAAGTTAAA
    GAAGTTAAAGCCCCTAAGGAAACAAAAGCAGTTAAACCAGCAGCAAAAGCCACTAACAAT
    ACATATCCTATTTTGAATCAGGAACTTAGAGAAGCGATTAAAAACCCTGCAATAAAAGAT
    AAAGATCATAGCGCACCAAACTCTCGTCCAATTGATTTTGAAATGAAAAAAGAAAATGGT
    GAGCAACAATTTTATCATTATGCCAGCTCTGTTAAACCTGCTAGAGTTATTTTCACTGAT
    TCAAAACCAGAAATTGAATTAGGATTACAATCAGGTCAATTTTGGAGAAAATTTGAAGTT
    TATGAAGGTGACAAAAAGTTGCCAATTAAATTAGTATCATACGATACTGTTAAAGATTAC
    GCTTACATTCGCTTCTCTGTTTCAAATGGAACAAAAGCCGTTAAAATTGTAAGTTCAACT
    CACTTCAATAACAAAGAAGAAAAATACGATTACACATTAATGGAATTCGCACAACCAATT
    TATAACAGTGCAGATAAATTCAAAACTGAAGAAGATTATAAAGCTGAAAAATTATTAGCG
    CCATATAAAAAAGCGAAAACACTAGAAAGACAAGTTTATGAATTAAATAAAATTCAAGAT
    AAACTTCCTGAAAAATTAAAGGCTGAGTACAAGAAGAAATTAGAGGATACAAAGAAAGCT
    TTAGATGAGCAAGTGAAATCAGCTATTACTGAATTCCAAAATGTACAACCAACAAATGAA
    AAAATGACTGATTTACAAGATACAAAATATGTTGTTTATGAAAGTGTTGAGAATAACGAA
    TCTATGATGGATACTTTTGTTAAACACCCTATTAAAACAGGTATGCTTAACGGCAAAAAA
    TATATGGTCATGGAAACTACTAATGACGATTACTGGAAAGATTTCATGGTTGAAGGTCAA
    CGTGTTAGAACTATAAGCAAAGATGCTAAAAATAATACTAGAACAATTATTTTCCCATAT
    GTTGAAGGTAAAACTCTATATGATGCTATCGTTAAAGTTCACGTAAAAACGATTGATTAT
    GATGGACAATACCATGTCAGAATCGTTGATAAAGAAGCATTTACAAAAGCCAATACCGAT
    AAATCTAACAAAAAAGAACAACAAGATAACTCAGCTAAGAAGGAAGCTACTCCAGCTACG
    CCTAGCAAACCAACACCATCACCTGTTGAAAAAGAATCACAAAAACAAGACAGCCAAAAA
    GATGACAATAAACAATTACCAAGTGTTGAAAAAGAAAATGACGCATCTAGTGAGTCAGGT
    AAAGACAAAACGCCTGCTACAAAACCAACTAAAGGTGAAGTAGAATCAAGTAGTACAACT
    CCAACTAAGGTAGTATCTACGACTCAAAATGTTGCAAAACCAACAACTGCTTCATCAAAA
    ACAACAAAAGATGTTGTTCAAACTTCAGCAGGTTCTAGCGAAGCAAAAGATAGTGCTCCA
    TTACAAAAAGCAAACATTAAAAACACAAATGATGGACACACTCAAAGCCAAAACAATAAA
    AATACACAAGAAAATAAAGCAAAATCATTACCACAAACTGGTGAAGAATCAAATAAAGAT
    ATGACATTACCATTAATGGCATTACTAGCTTTAAGTAGCATCGTTGCATTCGTATTACCT
    AGAAAACGTAAAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1938

⊟Protein[edit | edit source]

Protein Data Bank: 3RTL
Protein Data Bank: 3RUR

⊟General[edit | edit source]

  • locus tag: SA0976 [new locus tag: SA_RS05535 ]
  • symbol: IsdB
  • description: hypothetical protein
  • length: 645
  • theoretical pI: 9.55952
  • theoretical MW: 72161.6
  • GRAVY: -0.938605

⊟Function[edit | edit source]

  • TIGRFAM:
    heme uptake protein IsdB (TIGR03657; HMM-score: 1482.5)
    and 4 more
    haptoglobin-binding heme uptake protein HarA (TIGR03658; HMM-score: 525.3)
    heme uptake protein IsdC (TIGR03656; HMM-score: 41.9)
    gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 39)
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 18.2)
  • TheSEED  :
    • Cell surface receptor IsdB for hemoglobin and hemoglobin-haptoglobin complexes
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Cell surface receptor IsdB for hemoglobin and hemoglobin-haptoglobin complexes
  • PFAM:
    no clan defined NEAT; Iron Transport-associated domain (PF05031; HMM-score: 95.2)
    and 3 more
    YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 34.7)
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 32.9)
    Proton_antipo_C; NADH-dehyrogenase subunit F, TMs, (complex I) C-terminus (PF01010; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: found LPxTG motif :LPQTG
  • SignalP: Signal peptide SP(Sec/SPI) length 40 aa
    • SP(Sec/SPI): 0.958209
    • TAT(Tat/SPI): 0.037585
    • LIPO(Sec/SPII): 0.001797
    • Cleavage Site: CS pos: 40-41. AQA-AA. Pr: 0.6565
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAAEETGGTNTEAQPKTEAVASPTTTSEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEVKAPKETKAVKPAAKATNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKENGEQQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVKDYAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAEKLLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQNVQPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWKDFMVEGQRVRTISKDAKNNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVDKEAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPSVEKENDASSESGKDKTPATKPTKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVVQTSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLPLMALLALSSIVAFVLPRKRKN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Yukiko K Stranger-Jones, Taeok Bae, Olaf Schneewind
Vaccine assembly from surface proteins of Staphylococcus aureus.
Proc Natl Acad Sci U S A: 2006, 103(45);16942-7
[PubMed:17075065] [WorldCat.org] [DOI] (P p)Catherine F M Gaudin, Jason C Grigg, Angelé L Arrieta, Michael E P Murphy
Unique heme-iron coordination by the hemoglobin receptor IsdB of Staphylococcus aureus.
Biochemistry: 2011, 50(24);5443-52
[PubMed:21574663] [WorldCat.org] [DOI] (I p)

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