PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1020 [new locus tag: SA_RS05815 ]
• pan locus tag^?: SAUPAN003446000
• symbol: SA1020
• pan gene symbol^?: bshC
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1020 [new locus tag: SA_RS05815 ]
• symbol: SA1020
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1154519..1156132
• length: 1614
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123851 NCBI
• RefSeq: NP_374293 NCBI
• BioCyc: see SA_RS05815
• MicrobesOnline: 103319 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGACTGTAAAGTAGTTAGTTTAAATGAAAAAGATCAGTTTATACCAAAAATAAAGAGC
  AGTGACCCTGTAATAACAGGATTATTTCAATATGATGCAGCTCAACAAACTAGTTTTGAA
  AAAAGGATGTCTAAAGAAAATAATGGAAGAGAAGCGGCATTAGCGAATGTTATTCGTGAA
  TATATGAGTGATTTAAAGCTTTCAAATGAACAAGAATTAAACATACAACATTTAGCTAAT
  GGTTCAAAAGTTGTGATTGGTGGACAACAAGCAGGGCTTTTCGGGGGACCATTGTATACA
  TTCCATAAAATATTTTCAATCATTACTTTATCTAAGGAATTAACGGATACACATAAGCAA
  CAAGTAGTACCAGTTTTTTGGATTGCAGGAGAAGATCATGATTTCGATGAAGTGAATCAT
  ACATTTGTTTATAACGAAAATCATGGGTCGCTGCATAAGGTTAAATATCATACAATGGAG
  ATGCCAGAGACGACAGTCTCTAGATATTATCCTGATAAGGCTGAGTTGAAACAAACTTTA
  AAAACGATGTTCATTCATATGAAAGAAACTGTTCATACACAAGGTCTACTGGAGATTTGT
  GACAGAATTATTGACCAATATGACTCGTGGACTGATATGTTTAAAGCACTACTGCATGAA
  ACATTTAAAGCATATGGCGTTCTATTTATAGATGCGCAGTTTGAGCCGTTAAGAAAAATG
  GAAGCGCCTATGTTTAAAAAGATTTTGAAAAAACATCAGTTGCTTGATGATGCTTTTAGA
  GCAACACAACAACGTACTCAAAATCAAGGCTTGAATGCGATGATACAAACAGATACAAAT
  GTTCATTTATTCTTACATGATGAAAATATGCGCCAATTAGTTTCGTATGATGGTAAGCAT
  TTTAAATTAAATAAAACAGATAAGACATATATAAAGGAAGAAATTATAAATATTGCGGAA
  AATCAACCTGAATTATTTTCTAATAATGTAGTGACAAGACCATTAATGGAAGAATGGTTA
  TTTAACACGGTGGCATTTGTTGGAGGACCGAGTGAAATTAAGTACTGGGCTGAACTAAAA
  GATGTATTTGAACTATTTGATGTTGAAATGCCTATCGTGATGCCAAGGCTTAGAATTACT
  TATTTAAATGACCGTATAGAAAAATTACTTTCGAAATACAATATTCCATTAGAAAAAGTG
  TTAGTCGATGGTGTTGAAGGAGAAAGAAGTAAGTTTATTAGAGAACAAGCATCACATCAA
  TTTATTGAAAAGGTAGAAGGTATGATTGAACAACAGCGTCGTCTAAACAAAGACTTATTA
  GATGAAGTGGCGGGGAATCAAAATAATATTAACCTTGTGAATAAAAATAATGAAATTCAT
  ATACAACAGTATGATTATTTGTTAAAACGTTATCTTTTAAACATTGAAAGAGAAAACGAC
  ATCAGTATGAAGCAATTTAGAGAAATTCAAGAAACACTCCATCCAATGGGAGGATTACAA
  GAAAGAATATGGAATCCACTTCAAATTTTGAATGATTTTGGGACAGATGTGTTCAAGCCC
  TCCACCTATCCACCACTTTCTTACACTTTTGATCATATTATTATAAAACCTTAA
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⊟Protein

⊟General

• locus tag: SA1020 [new locus tag: SA_RS05815 ]
• symbol: SA1020
• description: hypothetical protein
• length: 537
• theoretical pI: 6.12147
• theoretical MW: 62833.4
• GRAVY: -0.494227

⊟Function

• reaction:
  EC 6.-.-.-?  ExPASy
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs bacillithiol biosynthesis cysteine-adding enzyme BshC (TIGR03998; HMM-score: 627.7)
• TheSEED  :
  • Glucosaminyl-malate:cysteine ligase
• PFAM:
  HUP (CL0039) BshC; Bacillithiol biosynthesis BshC (PF10079; HMM-score: 523.8)
  and 1 more
  no clan defined Minor_capsid_1; Minor capsid protein (PF10665; HMM-score: 13.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004053
  • TAT(Tat/SPI): 0.00031
  • LIPO(Sec/SPII): 0.000603
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926760 NCBI
• RefSeq: NP_374293 NCBI
• UniProt: Q7A621 UniProt

⊟Protein sequence

• MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMSKENNGREAALANVIREYMSDLKLSNEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFHKIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTMEMPETTVSRYYPDKAELKQTLKTMFIHMKETVHTQGLLEICDRIIDQYDSWTDMFKALLHETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQTDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRPLMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSKYNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNKDLLDEVAGNQNNINLVNKNNEIHIQQYDYLLKRYLLNIERENDISMKQFREIQETLHPMGGLQERIWNPLQILNDFGTDVFKPSTYPPLSYTFDHIIIKP

⊟Experimental data

• experimentally validated: data available for COL
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications