From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1047 [new locus tag: SA_RS05950 ]
  • pan locus tag?: SAUPAN003484000
  • symbol: pyrF
  • pan gene symbol?: pyrF
  • synonym:
  • product: orotidine 5'-phosphate decarboxylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1047 [new locus tag: SA_RS05950 ]
  • symbol: pyrF
  • product: orotidine 5'-phosphate decarboxylase
  • replicon: chromosome
  • strand: +
  • coordinates: 1186414..1187106
  • length: 693
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAAGATTTACCAATTATTGCATTAGATTTTGAATCAAAAGAAAAAGTAAATCAATTT
    TTAGATTTATTTGATGAATCATTATTCGTAAAAGTAGGTATGGAACTTTTTTATCAAGAA
    GGTCCTCAATTAATTAATGAGATAAAAGAAAGAGGCCATGATGTATTTTTAGATTTAAAA
    CTGCATGATATTCCTAATACAGTTGGTAAGGCGATGGAAGGACTAGCTAAATTGAATGTT
    GATCTGGTAAATGTTCATGCTGCTGGTGGCGTAAAAATGATGTCTGAGGCCATTAAAGGA
    TTAAGAAAACATAATCAACATACAAAAATTATTGCAGTAACACAGCTTACGTCAACAACA
    GAAGACATGTTACGACACGAACAAAATATACAAACATCGATTGAAGAGGCCGTTTTAAAT
    TATGCCAAGTTAGCAAATGCAGCTGGTTTAGATGGCGTTGTTTGTTCACCTCTTGAAAGT
    CGTATGTTGACTGAAAAGTTAGGTACATCATTTTTAAAAGTAACACCAGGTATTAGACCT
    AAAGGTGCATCTCAAGATGACCAACACCGTATTACGACACCGGAAGAAGCAAGACAGCTT
    GGTTCGACGCATATTGTAGTCGGTAGACCGATTACACAAAGTGACAATCCAGTCGAAAGT
    TATCATAAAATTAAAGAAAGTTGGTTAGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    693

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1047 [new locus tag: SA_RS05950 ]
  • symbol: PyrF
  • description: orotidine 5'-phosphate decarboxylase
  • length: 230
  • theoretical pI: 6.2391
  • theoretical MW: 25619.2
  • GRAVY: -0.292174

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.1.23?  ExPASy
    Orotidine-5'-phosphate decarboxylase Orotidine 5'-phosphate = UMP + CO2
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotidine 5'-phosphate decarboxylase (TIGR01740; EC 4.1.1.23; HMM-score: 207.2)
    and 1 more
    3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 22.3)
  • TheSEED  :
    • Orotidine 5'-phosphate decarboxylase (EC 4.1.1.23)
    Nucleosides and Nucleotides Pyrimidines De Novo Pyrimidine Synthesis  Orotidine 5'-phosphate decarboxylase (EC 4.1.1.23)
  • PFAM:
    TIM_barrel (CL0036) OMPdecase; Orotidine 5'-phosphate decarboxylase / HUMPS family (PF00215; HMM-score: 160.3)
    and 1 more
    no clan defined DUF3842; Domain of unknown function (DUF3842) (PF12953; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000682
    • TAT(Tat/SPI): 0.000081
    • LIPO(Sec/SPII): 0.000126
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQLINEIKERGHDVFLDLKLHDIPNTVGKAMEGLAKLNVDLVNVHAAGGVKMMSEAIKGLRKHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLESRMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPVESYHKIKESWLV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: pyrR leader (transcription antitermination) regulon
    pyrR leader(RNA)important in Pyrimidine metabolism; RegPrecise binding of the PyrR antitermination protein

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA1047&oldid=71106"