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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1214 [new locus tag: SA_RS06895 ]
  • pan locus tag?: SAUPAN003787000
  • symbol: opp-2B
  • pan gene symbol?: nikB
  • synonym: opp2B
  • product: oligopeptide transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1214 [new locus tag: SA_RS06895 ]
  • symbol: opp-2B
  • product: oligopeptide transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 1386698..1387684
  • length: 987
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTTCATAATAAAGTCTATGTTGTATAGACTGATGCAAATGATAGTTGTTTTATTTGTT
    ATTTCTACACTTACATTCATTTTAATGAAATTATCACCAGGCAATCCTGTTGATAAAATA
    TTACATCTTGATGTAGCACAAGTGTCGACGGAACAAATAAATGCAACCAAAGATAAATTG
    GGATTAAATGATTCTTTATTGGTACAGTGGTGGCATTGGATGAATCATTTATTACATTTT
    AATTTAGGAAAAAGTTTTGAATCAAAAGAACCGGTAACACAGATATTATTCAACTATGCA
    CCCATAACATTACTCATATCATTTTCAACATTAGTAGTGTCGTTATGCATATCTATACCG
    CTCGGCATTATAGCAGCCAAACGATTTCATAAGTGGACGGATAAAGTGATTAGAGTGATC
    TCAACACTATCGATTAGTTTACCAGCGTTCTTTATCGGTATTATTTTATTATTTATTGTC
    ACAAATTTAATGAATATAGATAGCGTTATACTAAGTCAGTTTATATTACCTGTAATCACG
    CTATCTTTAGGTATGTGTGCATACATCATTCGTTTAGTGCGTTCTAATTTATTGATGTTA
    TTGCAAAGTAATATCGTACAAGCATCAAGATTACGCGGTATGAATGAACGTTATATTTTA
    ATTCATGATTTACTAAAACCAACAATTTTGCCGATTATCCCATTACTAGGGATTTCACTT
    GGCAGTCTAATAGGTGGTACTGTAGTGATTGAAAATTTATTTGATATACCTGGTATTGGT
    TATCTATTAATGGATAGTATTAAATCTCGAGATTATCCTGTTATTCAAGGATGCGTGTTA
    TTTATTGGCTTCTTCGTTGTTATTATCAATACGATTGCTGATTTATTAACGTTATTACTT
    GATCCGAAGCAGCGTTTACAATTAGGAAATCCAAAAAACAAAACCAATACACCATTGATA
    TCAGAAAGTAGTGACCGTCATGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    987

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1214 [new locus tag: SA_RS06895 ]
  • symbol: Opp-2B
  • description: oligopeptide transporter permease
  • length: 328
  • theoretical pI: 9.77112
  • theoretical MW: 36815.1
  • GRAVY: 0.720732

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 227.4)
    and 3 more
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 27.2)
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 24.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 18.8)
  • TheSEED  :
    • ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines)
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 137.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.146125
    • TAT(Tat/SPI): 0.001088
    • LIPO(Sec/SPII): 0.016699
  • predicted transmembrane helices (TMHMM): 6

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFIIKSMLYRLMQMIVVLFVISTLTFILMKLSPGNPVDKILHLDVAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPITLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIVTNLMNIDSVILSQFILPVITLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERYILIHDLLKPTILPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQGCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKNKTNTPLISESSDRHA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Aurelia Hiron, Brunella Posteraro, Marie Carrière, Laetitia Remy, Cécile Delporte, Marilena La Sorda, Maurizio Sanguinetti, Vincent Juillard, Elise Borezée-Durant
A nickel ABC-transporter of Staphylococcus aureus is involved in urinary tract infection.
Mol Microbiol: 2010, 77(5);1246-60
[PubMed:20662775] [WorldCat.org] [DOI] (I p)

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