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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1537 [new locus tag: SA_RS08650 ]
  • pan locus tag?: SAUPAN004367000
  • symbol: SA1537
  • pan gene symbol?: thiI
  • synonym:
  • product: thiamine biosynthesis protein ThiI

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1537 [new locus tag: SA_RS08650 ]
  • symbol: SA1537
  • product: thiamine biosynthesis protein ThiI
  • replicon: chromosome
  • strand: -
  • coordinates: 1755646..1756869
  • length: 1224
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAAGTATGATCACTTGCTTGTTAGATACGGGGAGTTAACATTAAAGGGTTCAAATAGA
    AAAAAATTTGTAAATCAATTAAGAAATAATGTAAATAAGTCATTAAAAGGACTTGATGGG
    TTTGTCGTTAAAGGCAAACGAGATCGTATGTATATTGAACTTGAAGACCATGCAGATATA
    AATGAAATAACATATCGATTATCAAAAATTTTCGGTATTAAATCTATTAGTCCAGTATTA
    AAAGTAGAAAAAACAATAGAGGCAATGAGTGCAGCGGCAATTAAATTTGCGCAGCAATTT
    GAAGAAAACAGCACATTTAAAATTGATGTGAAGCGTGCCGATAAAAATTTCCCAATGGAT
    ACGTATGAATTACAACGTGAATTGGGTGGTACCGTATTGAAACAAATTGAAAATGTTTCT
    GTTAATGTCAAACGTGCAGATCATGAAATTCGAGTGGAAGTTAGATTAGATGCAATTTAT
    ATGTATGAAGAAGTTGTTCCGGGTTCAGGCGGATTACCAGTTGGTACTGGTGGTAAGACG
    TTACTAATGCTTTCAGGCGGTATAGACTCACCAGTTGCTGGAATGGAAGTGATGAGACGT
    GGCGTAACAATTGAAGCGATTCATTTCCATAGTCCACCATTTACAAGTGATCAAGCAAAA
    GAAAAAGTTATTGAATTGACACGTATTTTAGCTGAACGTGTTGGACCAATTAAATTGCAT
    ATTGTACCATTTACAGAATTGCAAAAACAGGTAAATAAAGTTGTACATCCAAGATATACA
    ATGACTTCAACGAGACGTATGATGATGCGTGTTGCTGATAAATTAGTACATCAAATAGGG
    GCTTTAGCTATTGTAAATGGTGAAAACCTAGGGCAGGTAGCCAGTCAAACACTTCATAGC
    ATGTATGCAATTAATAATGTAACTTCTACTCCTGTATTACGTCCTTTATTAACTTACGAT
    AAAGAAGAAATTATTATTAAATCGAAAGAAATTGGTACATTTGAAACATCTATTCAACCA
    TTTGAAGATTGTTGTACAATTTTCACCCCTAAAAATCCAGTAACCGAACCAAACTTTGAA
    AAGGTAGTCCAATATGAAAGTGTCTTTGATTTTGAAGAGATGATTAATCGTGCTGTTGAA
    AATATTGAAACACTTGAAATAACTAGTGATTATAAAACTATTAAAGAACAGCAAACAAAC
    CAATTAATAAATGACTTTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1224

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1537 [new locus tag: SA_RS08650 ]
  • symbol: SA1537
  • description: thiamine biosynthesis protein ThiI
  • length: 407
  • theoretical pI: 7.32256
  • theoretical MW: 46202
  • GRAVY: -0.281327

⊟Function[edit | edit source]

  • reaction:
    EC 2.8.1.4?  ExPASy
    tRNA uracil 4-sulfurtransferase ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP?
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 389.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine tRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 389.7)
    and 3 more
    Unknown function General queuosine biosynthesis protein QueC (TIGR00364; HMM-score: 21)
    Hypothetical proteins Conserved putative methyltransferase, TIGR01177 family (TIGR01177; HMM-score: 18.8)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 13.1)
  • TheSEED  :
    • tRNA 4-thiouridine(8) synthase (EC 2.8.1.4)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Cofactors, Vitamins, Prosthetic Groups, Pigments - no subcategory Thiamin biosynthesis  tRNA S(4)U 4-thiouridine synthase (former ThiI)
  • PFAM:
    HUP (CL0039) ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 205.5)
    and 3 more
    no clan defined THUMP; THUMP domain (PF02926; HMM-score: 59)
    HUP (CL0039) QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 19.9)
    ATP_bind_3; PP-loop family (PF01171; HMM-score: 14)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004641
    • TAT(Tat/SPI): 0.000294
    • LIPO(Sec/SPII): 0.000906
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAMSAAAIKFAQQFEENSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRADHEIRVEVRLDAIYMYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFEKVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTNQLINDFL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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