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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA1547 [new locus tag: SA_RS08720 ]
- pan locus tag?: SAUPAN004385000
- symbol: ptaA
- pan gene symbol?: ptaA
- synonym:
- product: PTS system N-acetylglucosamine-specific transporter subunit IIABC
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA1547 [new locus tag: SA_RS08720 ]
- symbol: ptaA
- product: PTS system N-acetylglucosamine-specific transporter subunit IIABC
- replicon: chromosome
- strand: -
- coordinates: 1768777..1770243
- length: 1467
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1124393 NCBI
- RefSeq: NP_374836 NCBI
- BioCyc: see SA_RS08720
- MicrobesOnline: 103862 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1441ATGTATAAATTTTTCCAAAATTTAGGGCGTTCGTTAATGTTACCAGTTGCCATTTTACCG
GCTGCAGCAATTATTGCCGGAATTGGTAATACGTTAAATGCACTTCATGCGACGCCGAAA
ATCGCAATGTTCTTTACTACGGTAGGTACTACGATTTTAGAGCAATTAGGTATTTTATTT
GCAATTGGTGTAGCTATAGGAATGGCAAAGAAAAATGATGGTGCAGTCGCGCTTGCAGCT
ACGTTAGGATATTTTTTAGTTACAGTTGTATTATCACCAATGAAATTAGCGCCTTTATTA
GGAATGAAAGCTTCGGAAATAAATTCAGCTTTTGAAAAGATGAATAATGGCAATGTCTTT
GTTGGTATTGTAATAGGTTTAATAGCAGCCTATGCATACAATAAGTTTAGTGAAACAGAA
TTACCATTAGCATTATCATTTTTTAGTGGTAAACGTCTAGTTCCAATTATGACTGCATTT
TACTGTACATTTTTAGTTGTCATATTGTTATTCTTATGGCCACTACTTTATTCATGGATT
GTAAAATTTGGTGAATCTATAGTCGGTTTAGGATCGTTTGGAGCTTTCATATATGGTGTT
GCCAATAGATTATTAATTCCTACAGGATTACATCATGCACTGAATAGTGTGTTCTGGTTT
GATACGATTGGTATTAATGATATTGGTAAATTCCAAAGTGGCAAAGATGCTATAAAAGGT
ATTACAGGACGCTATCAAGCGGGATTCTTCCCAATCATGATGTTCGGTATTCCAGCGGCT
GCGTTAGCAATGTATCATACAGCTAAAACGACTCAGAAAAAACAAGTTTACGGTTGGTTC
CTAGCGAGTTCAGTTGCAGCATTTTTCGTTGGTGTTACAGAGCCGATTGAGTTTGCATTT
ATGTTTGTTGCGCCAATCTTATATGTTGTTCATGCGTTATTAACAGGATTATCTTTATTT
ATCGCAGCTACATTCCATTGGACAGCTGGATTTTCATTTAGTGCAGGTTTGATAGATTAT
GTGTTATCACTAATTAATCCAGTATCAAATCATCCATTGATGTTGCTTGTTCAAGGTGTT
GTATTTTTCATCTTATACTATGTGATTTTCCGTGTAGTAATCCAAGTATTTAACTTGAAT
ACGATTGGTAGAGGTGAAAATGAATTAGTTGATCCAACAGTTGTAAAAGATAATATTGCT
CCTGGTGAAAATGATATTAAACAAAGTAAATATCATCAACATGCTATACAAATATTAGAA
GGTTTAGGCGGTCAAGAGAATATTGTTAATTTAACCAATTGTGCAACAAGGTTGCGTCTA
GAGTTAAAAGACACATCTATTATTGATCAACAGAAGATTAAAAACGCCGGTGCAGTTGGT
GTTACTGTTAATGGCAAACATTCAACTCAAGTTATTGTCGGTACACACGTACAGCAGGTT
GCTGATGAAATTGAAAAACATTTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA1547 [new locus tag: SA_RS08720 ]
- symbol: PtaA
- description: PTS system N-acetylglucosamine-specific transporter subunit IIABC
- length: 488
- theoretical pI: 9.24794
- theoretical MW: 53086.2
- GRAVY: 0.626025
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 715.3)and 11 moreTransport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 380.9)Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 292.8)Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 231.4)Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 197)Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 176.1)Signal transduction PTS PTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 176.1)PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 58)PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 55.8)Transport and binding proteins Carbohydrates, organic alcohols, and acids PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 46.4)Signal transduction PTS PTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 46.4)PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 44.7)
- TheSEED :
- PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.193)
- PTS system, N-acetylglucosamine-specific IIC component
Carbohydrates Aminosugars Chitin and N-acetylglucosamine utilization PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69)and 3 moreCarbohydrates Aminosugars Chitin and N-acetylglucosamine utilization PTS system, N-acetylglucosamine-specific IIC component - PFAM: PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 233.1)and 3 moreno clan defined PTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 57.2)Oxa1 (CL0376) 60KD_IMP; 60Kd inner membrane protein (PF02096; HMM-score: 8.8)no clan defined SH; Viral small hydrophobic protein (PF01445; HMM-score: 8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 11
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: 0.5
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.213941
- TAT(Tat/SPI): 0.014522
- LIPO(Sec/SPII): 0.102848
- predicted transmembrane helices (TMHMM): 11
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMFFTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAATLGYFLVTVVLSPMKLAPLLGMKASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAFYCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVFWFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQVYGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFSAGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDPTVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQQKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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