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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA1585 [new locus tag: SA_RS08935 ]
- pan locus tag?: SAUPAN004454000
- symbol: SA1585
- pan gene symbol?: putA
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA1585 [new locus tag: SA_RS08935 ]
- symbol: SA1585
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 1823844..1824845
- length: 1002
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1124431 NCBI
- RefSeq: NP_374874 NCBI
- BioCyc: see SA_RS08935
- MicrobesOnline: 103900 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGGCACTATTAAAGAATTTTTTTATCGGATTATCTAATAATAGTTTTTTAAACAACGCA
GCAAAAAAAGTGGGCCCACGTTTGGGCGCCAATAAAGTCGTTGCCGGAAATACAATTCCA
GAGTTAATTAATACAATCGAATACTTAAATGACAAGAATATCGCTGTTACGGTAGACAAT
TTAGGGGAATTTGTCGGTACAGTTGAAGAAAGTAATCATGCTAAAGAACAAATTTTAACA
ATTATGGACGCGCTTCATCAACATGGCGTAAAGGCACATATGTCTGTTAAATTGAGTCAG
TTAGGTGCAGAATTCGACTTAGAATTAGCTTACCAAAATTTAAGAGAAATTTTACTTAAA
GCAAATACTTACAACAATATGCATATAAATATTGATACTGAAAAATATGCTAGCCTGCAA
CAAATTGTTCAAGTTTTAGATCGCTTAAAAGGCGAATTTAGAAATGTTGGTACTGTAATT
CAAGCATATTTATACGATAGCCACAAATTAGTTGATAAGTACCAAGATTTACGATTACGT
TTGGTTAAAGGTGCATATAAAGAAAACGAATCAATTGCATTTCAATCTAAGGAAGACGTA
GATGCAAATTACATCAAAATAATTGAACAACGTTTGTTAAACGCACGCAATTTCACTTCA
ATTGCAACACATGACCATCGCATCATTAATCATGTAAAACAATTTATGAAAGAAAATCAC
ATTGAAAAAGATCGTATGGAATTCCAAATGCTCTATGGTTTTAGATCAGAGTTAGCAGAA
GAAATCGCAAATGAAGGCTATAATTTCACTATTTATGTACCTTATGGCGATGATTGGTTT
GCGTATTTTATGAGAAGATTAGCAGAACGCCCACAAAACCTATCTCTTGCTGTAAAAGAA
TTTGTGAAACCTGCTGGCTTAAAACGTGTTGGCATAATTGCAGCTTTAGGAGCTACAGTT
ATGTTAGGTTTAAGTACAATTAAAAAATTATGCCGTAAATAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA1585 [new locus tag: SA_RS08935 ]
- symbol: SA1585
- description: hypothetical protein
- length: 333
- theoretical pI: 9.0278
- theoretical MW: 38028.5
- GRAVY: -0.245345
⊟Function[edit | edit source]
- TIGRFAM: acidobacterial duplicated orphan permease (TIGR03434; HMM-score: 15.3)
- TheSEED :
- Proline dehydrogenase (EC 1.5.5.2)
Amino Acids and Derivatives Proline and 4-hydroxyproline Proline, 4-hydroxyproline uptake and utilization Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase)and 1 more - PFAM: FAD_oxidored (CL0086) Pro_dh; Proline dehydrogenase (PF01619; HMM-score: 108.9)and 3 moreno clan defined PorB; Alpha helical Porin B (PF11565; HMM-score: 13.6)DUF3347; Protein of unknown function (DUF3347) (PF11827; HMM-score: 13)ExbD; Biopolymer transport protein ExbD/TolR (PF02472; HMM-score: 12.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: FAD
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- LocateP: Intracellular /TMH start AFTER 60
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: Possibly Sec-
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.015058
- TAT(Tat/SPI): 0.002094
- LIPO(Sec/SPII): 0.001817
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELINTIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLGAEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVIQAYLYDSHKLVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNFTSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYGDDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: CcpA regulon
CcpA (TF) important in Carbon catabolism; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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