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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1557 [new locus tag: SA_RS08780 ]
  • pan locus tag?: SAUPAN004400000
  • symbol: ccpA
  • pan gene symbol?: ccpA
  • synonym:
  • product: catabolite control protein A

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1557 [new locus tag: SA_RS08780 ]
  • symbol: ccpA
  • product: catabolite control protein A
  • replicon: chromosome
  • strand: -
  • coordinates: 1784194..1785138
  • length: 945
  • essential: yes DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGCCACAGTGTCGCGTGTTGTTAATGGGAACCAAAATGTTAAAGCAGAAACTAAAAAT
    AAAGTTAACGAAGTCATTAAGCGTTTGAATTATCGTCCAAATGCTGTTGCTAGAGGTTTA
    GCTAGTAAAAAGACAACAACAGTAGGTGTGATCATTCCAGATATATCTAATATCTATTAT
    TCACAACTTGCTCGTGGACTTGAAGATATTGCAACAATGTATAAATATCACTCAATTATT
    TCAAATTCAGATAACGATCCTGAAAAGGAAAAAGAAATTTTTAATAACTTATTAAGTAAA
    CAGGTTGATGGTATTATTTTCCTTGGTGGTACAATTACTGAAGAAATGAAAGAATTGATA
    AATCAATCATCTGTACCTGTAGTAGTATCAGGAACAAATGGTAAGGATGCACATATAGCA
    TCAGTTAATATTGATTTTACTGAAGCTGCGAAAGAAATTACGGGAGAATTAATTGAAAAA
    GGCGCTAAATCATTTGCTTTAGTAGGTGGAGAACATTCTAAAAAAGCTCAAGAAGATGTT
    TTAGAAGGTTTAACTGAAGTGTTAAATAAAAATGGCCTTCAATTAGGTGATACATTGAAT
    TGTTCTGGTGCTGAAAGTTATAAAGAAGGCGTAAAAGCTTTTGCTAAAATGAAAGGCAAT
    TTGCCAGATGCCATTTTATGTATCAGCGACGAAGAAGCAATTGGTATTATGCATAGTGCA
    ATGGATGCTGGTATTAAAGTTCCAGAGGAATTACAAATTATTAGTTTCAATAATACACGA
    TTAGTTGAGATGGTTAGACCACAACTTTCTAGTGTTATTCAACCATTATATGATATCGGT
    GCAGTAGGGATGCGCTTATTAACAAAATATATGAACGATGAAAAGATAGAAGAACCAAAT
    GTAGTTTTACCTCACAGAATTGAATACCGAGGAACTACAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    945

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1557 [new locus tag: SA_RS08780 ]
  • symbol: CcpA
  • description: catabolite control protein A
  • length: 314
  • theoretical pI: 5.4927
  • theoretical MW: 34367.1
  • GRAVY: -0.250637

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 536.4)
    and 2 more
    Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 146.4)
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 91.7)
  • TheSEED  :
    • Catabolite control protein A
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory HPr catabolite repression system  Catabolite control protein A
  • PFAM:
    Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 105.5)
    and 8 more
    Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 74.8)
    Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 71.2)
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 41.6)
    no clan defined ATP-synt_F; ATP synthase (F/14-kDa) subunit (PF01990; HMM-score: 16.4)
    4Fe-4S (CL0344) Fer4_12; 4Fe-4S single cluster domain (PF13353; HMM-score: 14.7)
    MurF-HprK_N (CL0365) Hpr_kinase_N; HPr Serine kinase N terminus (PF02603; HMM-score: 14.6)
    no clan defined DUF327; Protein of unknown function (DUF327) (PF03885; HMM-score: 14.4)
    NADP_Rossmann (CL0063) CoA_binding_2; CoA binding domain (PF13380; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005332
    • TAT(Tat/SPI): 0.000559
    • LIPO(Sec/SPII): 0.000661
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MATVSRVVNGNQNVKAETKNKVNEVIKRLNYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDPEKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNIDFTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSGAESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRLVEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)

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