PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1007 [new locus tag: SA_RS05730 ]
• pan locus tag^?: SAUPAN003418000
• symbol: SA1007
• pan gene symbol^?: hla
• synonym:
• product: alpha-hemolysin

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1007 [new locus tag: SA_RS05730 ]
• symbol: SA1007
• product: alpha-hemolysin
• replicon: chromosome
• strand: -
• coordinates: 1140562..1141521
• length: 960
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123836 NCBI
• RefSeq: NP_374279 NCBI
• BioCyc: see SA_RS05730
• MicrobesOnline: 103305 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAACACGTATAGTCAGCTCAGTAACAACAACACTATTGCTAGGTTCCATATTAATG
  AATCCTGTCGCTAATGCCGCAGATTCTGATATTAATATTAAAACCGGTACTACAGATATT
  GGAAGCAATACTACAGTAAAAACAGGTGATTTAGTCACTTATGATAAAGAAAATGGCATG
  CACAAAAAAGTATTTTATAGTTTTATCGATGATAAAAATCACAATAAAAAACTGCTAGTT
  ATTAGAACGAAAGGTACCATTGCTGGTCAATATAGAGTTTATAGCGAAGAAGGTGCTAAC
  AAAAGTGGTTTAGCCTGGCCTTCAGCCTTTAAGGTACAGTTGCAACTACCTGATAATGAA
  GTAGCTCAAATATCTGATTACTATCCAAGAAATTCGATTGATACAAAAGAGTATATGAGT
  ACTTTAACTTATGGATTCAACGGTAATGTTACTGGTGATGATACAGGAAAAATTGGCGGC
  CTTATTGGTGCAAATGTTTCGATTGGTCATACACTGAAATATGTTCAACCTGATTTCAAA
  ACAATTTTAGAGAGCCCAACTGATAAAAAAGTAGGCTGGAAAGTGATATTTAACAATATG
  GTGAATCAAAATTGGGGACCATATGATAGAGATTCTTGGAACCCGGTATATGGCAATCAA
  CTTTTCATGAAAACTAGAAATGGTTCTATGAAAGCAGCAGAGAACTTCCTTGATCCTAAC
  AAAGCAAGTTCTCTATTATCTTCAGGGTTTTCACCAGACTTCGCTACAGTTATTACTATG
  GATAGAAAAGCATCCAAACAACAAACAAATATAGATGTAATATACGAACGAGTTCGTGAT
  GACTACCAATTGCATTGGACTTCAACAAATTGGAAAGGTACCAATACTAAAGATAAATGG
  ACAGATCGTTCTTCAGAAAGATATAAAATCGATTGGGAAAAAGAAGAAATGACAAATTAA
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⊟Protein

⊟General

• locus tag: SA1007 [new locus tag: SA_RS05730 ]
• symbol: SA1007
• description: alpha-hemolysin
• length: 319
• theoretical pI: 9.18585
• theoretical MW: 35975.1
• GRAVY: -0.662069

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance beta-channel forming cytolysin (TIGR01002; HMM-score: 437.6)
  and 1 more
  CXXX repeat peptide modification system protein (TIGR04116; HMM-score: 12)
• TheSEED  :
  • Cytolytic pore-forming protein => Alpha-hemolysin
  Virulence, Disease and Defense Toxins and superantigens Staphylococcus Two-component and Pore-forming Cytolysins  Alpha-hemolysin precursor
• PFAM:
  Leukocidin (CL0636) Leukocidin; Leukocidin/Hemolysin toxin family (PF07968; HMM-score: 277.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 10
  • Internal Helices: 0
• LocateP: Secretory(released) (with CS)
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: -0.17
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -2
  • Predicted Cleavage Site: PVANAADS
• SignalP: Signal peptide SP(Sec/SPI) length 26 aa
  • SP(Sec/SPI): 0.997425
  • TAT(Tat/SPI): 0.00165
  • LIPO(Sec/SPII): 0.000505
  • Cleavage Site: CS pos: 26-27. ANA-AD. Pr: 0.9908
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926746 NCBI
• RefSeq: NP_374279 NCBI
• UniProt: A0A0H3JMC2 UniProt

⊟Protein sequence

• MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNTTVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKLLVIRTKGTIAGQYRVYSEEGANKSGLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDTGKIGGLIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYGNQLFMKTRNGSMKAAENFLDPNKASSLLSSGFSPDFATVITMDRKASKQQTNIDVIYERVRDDYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulators: CcpA regulon, SaeR (activation) regulon

  CcpA	(TF)	important in Carbon catabolism; RegPrecise
  SaeR	(TF)	important in Virulence; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Saumya S Srivastava, Satyabrata Pany, Amita Sneh, Neesar Ahmed, Aejazur Rahman, Krishnasastry V Musti
Membrane bound monomer of Staphylococcal alpha-hemolysin induces caspase activation and apoptotic cell death despite initiation of membrane repair pathway.
PLoS One: 2009, 4(7);e6293
[PubMed:19621082] [WorldCat.org] [DOI] (I e)Anne F Hammerstein, Lakmal Jayasinghe, Hagan Bayley
Subunit dimers of alpha-hemolysin expand the engineering toolbox for protein nanopores.
J Biol Chem: 2011, 286(16);14324-34
[PubMed:21324910] [WorldCat.org] [DOI] (I p)Takaki Sugawara, Daichi Yamashita, Yoshikazu Tanaka, Jun Kaneko, Yoshiyuki Kamio, Isao Tanaka, Min Yao
Preliminary X-ray crystallographic study of staphylococcal α-haemolysin monomer.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2013, 69(Pt 8);868-70
[PubMed:23908030] [WorldCat.org] [DOI] (I p)