PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2226 [new locus tag: SA_RS12780 ]
• pan locus tag^?: SAUPAN005986000
• symbol: SA2226
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2226 [new locus tag: SA_RS12780 ]
• symbol: SA2226
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2498051..2499091
• length: 1041
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 3251081 NCBI
• RefSeq: NP_375549 NCBI
• BioCyc: see SA_RS12780
• MicrobesOnline: 104575 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
  
  ATGGCAAGAAAATTGCATAGAGAGTTGAATAACAGACACATCCAATTGATAGCAATTGGG
  GGCGCAATTGGAACTGGGTTATTCCTAGGATCAGGTCAAACAATATCTTTAACTGGTCCA
  TCACTGTTATTCACATACATGATTATTGGGGTTGTACTATTCGCTTTTATGCGCGCATTA
  GGCGAATTGTTGTTGAGCAATACAAGATTTAATTCATTTGTTGATATTGCAAATGAATAT
  TTAGGCCCTTTTGGTGGCTTTGTCATTGGCTGGACTTACTGGTTATGTTGGATTGTATCA
  AGTATGTCAGACCTAACTGCGATGGGACAATACTTTGCATTTTGGTATCCACAAGTCCCA
  AATTGGATTACCGTGCTATTTATTGTTTTAATCTTGATTAGCTTCAACTTATTAGGTGCC
  AGATTATTTGGTGAACTGGAGTTTTGGTTCTCTATTATTAAAGTTGTCACAATTATTGCG
  ATGGTTATCGTTGGTCTTGTATTAATCTTTTTCTCATTTAAAACACATTATGGACATGCA
  TCATTCACAAACTTAATCAGTCACGGCGGCATGTTCCCTGGAGGAACATTTGGTTTCCTA
  ATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCT
  GGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGT
  ATTTTATTATTCTATATCGGTGGTCTATTAGTAATTATGTCAGTCATACCTTGGAATGAT
  ATCGATCCAAATAGTAGCCCTTTCGTTAAACTCTTTACATTAATCGGCGTGCCATTTGCA
  GCAGGCGTCGTTAACTTTGTCGTGCTAACTGCCGCGGCCTCTGCTACAAATAGTGGTATC
  TATTCGAATAGTCGTATCTTATTCGGACTGTCACAACAAGGGTTAGGTCCTAAAGTTTTA
  AATTGGCGTGCCTTATTTATCAATGTTAGTTTCATCAATTGCATTACTTATAGCAGCCTT
  GTTAAACTACATTTTCCCTAA
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  1041
  

⊟Protein

⊟General

• locus tag: SA2226 [new locus tag: SA_RS12780 ]
• symbol: SA2226
• description: hypothetical protein
• length: 346
• theoretical pI: 9.47179
• theoretical MW: 38143.9
• GRAVY: 0.815029

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 274)
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 236.5)
  and 4 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 105.2)
  Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 68.3)
  Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 62.3)
  histidine-histamine antiporter (TIGR04298; HMM-score: 52.9)
• TheSEED  :
  • D-serine/D-alanine/glycine transporter
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  D-serine/D-alanine/glycine transporter
  and 1 more
  Carbohydrates Central carbohydrate metabolism Pyruvate Alanine Serine Interconversions  D-serine/D-alanine/glycine transporter
• PFAM:
  APC (CL0062) AA_permease; Amino acid permease (PF00324; HMM-score: 282.1)
  and 2 more
  AA_permease_2; Amino acid permease (PF13520; HMM-score: 118.7)
  Spore_permease; Spore germination protein (PF03845; HMM-score: 23.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 8
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.015444
  • TAT(Tat/SPI): 0.004814
  • LIPO(Sec/SPII): 0.083446
• predicted transmembrane helices (TMHMM): 9

⊟Accession numbers

• GI: 15928016 NCBI
• RefSeq: NP_375549 NCBI
• UniProt: A0A0H3JNF3 UniProt

⊟Protein sequence

• MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLFTYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSMSDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIAMVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGVTAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIGVPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNWRALFINVSFINCITYSSLVKLHFP

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA2226 > SA2227 > SA2228

⊟Regulation

• regulators: CcpA regulon, CodY (repression) regulon

  CcpA	(TF)	important in Carbon catabolism; RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications