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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2226 [new locus tag: SA_RS12780 ]
  • pan locus tag?: SAUPAN005986000
  • symbol: SA2226
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA2226 [new locus tag: SA_RS12780 ]
  • symbol: SA2226
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2498051..2499091
  • length: 1041
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGCAAGAAAATTGCATAGAGAGTTGAATAACAGACACATCCAATTGATAGCAATTGGG
    GGCGCAATTGGAACTGGGTTATTCCTAGGATCAGGTCAAACAATATCTTTAACTGGTCCA
    TCACTGTTATTCACATACATGATTATTGGGGTTGTACTATTCGCTTTTATGCGCGCATTA
    GGCGAATTGTTGTTGAGCAATACAAGATTTAATTCATTTGTTGATATTGCAAATGAATAT
    TTAGGCCCTTTTGGTGGCTTTGTCATTGGCTGGACTTACTGGTTATGTTGGATTGTATCA
    AGTATGTCAGACCTAACTGCGATGGGACAATACTTTGCATTTTGGTATCCACAAGTCCCA
    AATTGGATTACCGTGCTATTTATTGTTTTAATCTTGATTAGCTTCAACTTATTAGGTGCC
    AGATTATTTGGTGAACTGGAGTTTTGGTTCTCTATTATTAAAGTTGTCACAATTATTGCG
    ATGGTTATCGTTGGTCTTGTATTAATCTTTTTCTCATTTAAAACACATTATGGACATGCA
    TCATTCACAAACTTAATCAGTCACGGCGGCATGTTCCCTGGAGGAACATTTGGTTTCCTA
    ATGTCATTCCAAATTGCTGTATATTCATTCATTGGTATTGAACTTATAGGTGTAACTGCT
    GGTGAAACGAAAGATCCTGAAAAAACCTTACCGAAAGCAATTAATAATGTACCTATCCGT
    ATTTTATTATTCTATATCGGTGGTCTATTAGTAATTATGTCAGTCATACCTTGGAATGAT
    ATCGATCCAAATAGTAGCCCTTTCGTTAAACTCTTTACATTAATCGGCGTGCCATTTGCA
    GCAGGCGTCGTTAACTTTGTCGTGCTAACTGCCGCGGCCTCTGCTACAAATAGTGGTATC
    TATTCGAATAGTCGTATCTTATTCGGACTGTCACAACAAGGGTTAGGTCCTAAAGTTTTA
    AATTGGCGTGCCTTATTTATCAATGTTAGTTTCATCAATTGCATTACTTATAGCAGCCTT
    GTTAAACTACATTTTCCCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1041

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA2226 [new locus tag: SA_RS12780 ]
  • symbol: SA2226
  • description: hypothetical protein
  • length: 346
  • theoretical pI: 9.47179
  • theoretical MW: 38143.9
  • GRAVY: 0.815029

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 274)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid permease (yeast) (TIGR00913; HMM-score: 236.5)
    and 4 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino acid transporter (TIGR00909; HMM-score: 105.2)
    Metabolism Transport and binding proteins Amino acids, peptides and amines ethanolamine permease (TIGR00908; HMM-score: 68.3)
    Metabolism Transport and binding proteins Amino acids, peptides and amines cationic amino acid transport permease (TIGR00906; HMM-score: 62.3)
    histidine-histamine antiporter (TIGR04298; HMM-score: 52.9)
  • TheSEED:  
    Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  D-serine/D-alanine/glycine transporter
    and 1 more
    Carbohydrates Central carbohydrate metabolism Pyruvate Alanine Serine Interconversions  D-serine/D-alanine/glycine transporter
  • PFAM:
    APC (CL0062) AA_permease; Amino acid permease (PF00324; HMM-score: 282.1)
    and 2 more
    AA_permease_2; Amino acid permease (PF13520; HMM-score: 118.7)
    Spore_permease; Spore germination protein (PF03845; HMM-score: 23.3)

Structure, modifications & interactions[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015444
    • TAT(Tat/SPI): 0.004814
    • LIPO(Sec/SPII): 0.083446
  • predicted transmembrane helices (TMHMM): 9

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MARKLHRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLFTYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSMSDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIAMVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGVTAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIGVPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNWRALFINVSFINCITYSSLVKLHFP

Experimental data[edit | edit source]

  • experimentally validated: no data available

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulators: CcpA regulon, CodY (repression) regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise  
    CodY(TF)important in Amino acid metabolism; RegPrecise  

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]