PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2227
• pan locus tag^?: SAUPAN005987000
• symbol: SA2227
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2227
• symbol: SA2227
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2499033..2499446
• length: 414
• essential: no DEG

⊟Accession numbers

• Gene ID: 1125154 NCBI
• RefSeq: NP_375550 NCBI
• BioCyc:
• MicrobesOnline: 104576 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  
  ATGTTAGTTTCATCAATTGCATTACTTATAGCAGCCTTGTTAAACTACATTTTCCCTAAT
  GCAATTCAACTATTCATATACGTTACAACGTTATCAACTGTGTTGTTTTTAGTTGTTTGG
  GCAATGATTATTGTCGCTTATCTAATGTATTTGAAAAAGCATCCTGAGGCACATAAAAAC
  AGTAAATTTAAGTTGATTGGTGGTAAGCCTATTGCTTACATTATTCTAGCGTTCTTCTTC
  TTTGTATTCATTTTATTATTCTTTAGTGACGAAACAAGAGCAGCTATTTATATTTCACCA
  TTCTGGTTTATATTTTTATTTTTCTTTTATAAAAAATACAAAACGAATGCTGAAAAGCTC
  GCGTATGAACAACGACAAAATGACAGCGGTCATTTCAGATATGACAATCAATAA
  60
  120
  180
  240
  300
  360
  414
  

⊟Protein

⊟General

• locus tag: SA2227
• symbol: SA2227
• description: hypothetical protein
• length: 137
• theoretical pI: 9.9069
• theoretical MW: 16199.2
• GRAVY: 0.641606

⊟Function

• TIGRFAM:
  undecaprenyl-phosphate glucose phosphotransferase (TIGR03023; EC 2.7.8.-; HMM-score: 12.8)
  Metabolism Transport and binding proteins Amino acids, peptides and amines GABA permease (TIGR01773; HMM-score: 10.5)
  Metabolism Energy metabolism Electron transport cytochrome c oxidase, subunit II (TIGR02866; EC 1.9.3.1; HMM-score: 10.4)
  and 1 more
  exopolysaccharide biosynthesis polyprenyl glycosylphosphotransferase (TIGR03025; HMM-score: 10.2)
• TheSEED  :
  • D-serine/D-alanine/glycine transporter
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  D-serine/D-alanine/glycine transporter
  and 1 more
  Carbohydrates Central carbohydrate metabolism Pyruvate Alanine Serine Interconversions  D-serine/D-alanine/glycine transporter
• PFAM:
  APC (CL0062) AA_permease; Amino acid permease (PF00324; HMM-score: 28.8)
  and 16 more
  AA_permease_2; Amino acid permease (PF13520; HMM-score: 18.6)
  no clan defined Oxidored_q3; NADH-ubiquinone/plastoquinone oxidoreductase chain 6 (PF00499; HMM-score: 16.8)
  DUF4500; Domain of unknown function (DUF4500) (PF14937; HMM-score: 14.1)
  Pho86; Inorganic phosphate transporter Pho86 (PF11124; HMM-score: 12.8)
  RAMP4; Ribosome associated membrane protein RAMP4 (PF06624; HMM-score: 11.5)
  Transporter (CL0375) Amastin; Amastin surface glycoprotein (PF07344; HMM-score: 11.4)
  no clan defined CobS; Cobalamin-5-phosphate synthase (PF02654; HMM-score: 10.4)
  Gx_transp (CL0315) ECF_trnsprt; ECF transporter, substrate-specific component (PF12822; HMM-score: 10)
  Peroxisome (CL0484) PRT_C; Plant phosphoribosyltransferase C-terminal (PF08372; HMM-score: 8.5)
  Peptidase_MA (CL0126) Peptidase_M50B; Peptidase M50B-like (PF13398; HMM-score: 8.5)
  no clan defined IncA; IncA protein (PF04156; HMM-score: 8.3)
  DUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 8.3)
  NfeD-like (CL0252) NfeD; NfeD-like C-terminal, partner-binding (PF01957; HMM-score: 7.4)
  no clan defined DHHC; DHHC palmitoyltransferase (PF01529; HMM-score: 6.6)
  Halogen_Hydrol; 5-bromo-4-chloroindolyl phosphate hydrolysis protein (PF10112; HMM-score: 6.1)
  DUF3043; Protein of unknown function (DUF3043) (PF11241; HMM-score: 6.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 4
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.000585
  • TAT(Tat/SPI): 0.000135
  • LIPO(Sec/SPII): 0.006131
• predicted transmembrane helices (TMHMM): 4

⊟Accession numbers

• GI: 15928017 NCBI
• RefSeq: NP_375550 NCBI
• UniProt: A0A0H3JMR1 UniProt

⊟Protein sequence

• MLVSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKNSKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAEKLAYEQRQNDSGHFRYDNQ

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA2226 > SA2227 > SA2228

⊟Regulation

• regulators: CcpA regulon, CodY (repression) regulon

  CcpA	(TF)	important in Carbon catabolism; RegPrecise
  CodY	(TF)	important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications