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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2294 [new locus tag: SA_RS13155 ]
  • pan locus tag?: SAUPAN006131000
  • symbol: gntK
  • pan gene symbol?: gntK
  • synonym:
  • product: gluconokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2294 [new locus tag: SA_RS13155 ]
  • symbol: gntK
  • product: gluconokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2576949..2578502
  • length: 1554
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAAATATATGATTGGTGTCGACATTGGAACGACGAGTACCAAGTCAGTCTTATATGAT
    GAAAATGGAGCTTTTATCATGAAACATCAAATCGGCTATGATTTACACACACCAAACGTT
    GATGTCTCAGAAGAAAACCCAGATGAATTATTTGATGCGGTATTAATGACAATTAAATAC
    ATAATGAGGGAATCGAAAGTTAATCAAGATGATATTAAATTTGTGTCATTTAGTGCGCAA
    ATGCATAGCTTGATTGCGATGGATCAGCAACATCAAAGATTAACAAATAATATTACTTGG
    GCAGATAACCGCGCTGCAAAATATGCAACAGTAATAAATGAAGTGCATGATGGCAATGCG
    ATTTATCAGCGAACAGGTACGCCTATTCATCCTATGTCGCCTTTAGTGAAAATTTTTTGG
    ATGAAACATGAATGGCAAGATGTATTTCAACGTACTGCTAAGTTTGCAGATATTAAAACA
    TACATTTTCTATCATTTATTTGATACATATATCATTGATTATTCAATGGCTTCCGCAACA
    GGGATGTTTAATTTAGAAACATTAGATTGGGATGTTGGGGCATTAGAATTGCTTGGTATT
    TCCAAGGAAATGTTCCCGGAATTAGTGCCAACAACGTACGTAATGAAAGGCATGAAAGAA
    CGTTATGCAACATTAATGGGGCTTAATGAAGATACACCGTTTGTTATTGGTGCGAGTGAT
    GGGGTCCTTTCTAATTTGGGTGTCAATAGTGTTGGTAAAGGAGAAGTTGCTGTCACAATC
    GGTACATCTGGCGCGATTCGTACTGTGATAGATAAACCACGTACTGATTACAAAGGTAGA
    ATATTTTGTTATGTCTTAACAGAGGACCACTACGTCATCGGAGGTCCTGTAAATAATGGT
    GGTGTCGTATTGAGATGGTTGCGCGACGAGTTGCTAGCGAGTGAAGTCGAAACTGCGAAA
    CGTCTCGGTGTTGATCCTTATGATGTCTTAACTCAAATTGCAAAGCGTGTTAAACCAGGT
    GCAGATGGTTTAATATTCCATCCCTATTTAGCTGGAGAACGTGCGCCGCTTTGGAACGCA
    AATGCAAGAGGTTCATTCTTCGGTTTAACTTTATCTCATAAAAAAGAACATATGATTCGC
    GCTGCATTAGAAGGTGTTCTTTACAATTTGTATACCGTCTACCTTGCACTCATTGAAGTA
    ATGAATGAAACGCCTAATATGATAAAAGCAACAGGTGGTTTTGCGAAAAGTGAAGTATGG
    CGTCAAATGATGTCAGATATATTTGACACAGAGTTAGTGGTTCCTGAAAGTTATGAAAGT
    TCATGCTTAGGTGCCTGCGTGCTTGGACTTAAAGCTGTAGGTGACATTGAAGATTTTTCA
    ATCGTTTCATCGATGGTCGGTGCTACAAATAATCATACGCCGTTTGAAGAAAATGTCGCT
    GTTTACCAAGAGCTCGTATCCATTTTTATCAATTTAAGTCGTTCTTTAACAGAGAATTAT
    GAACAAATTGCAGATTTTCAACGCCAACATATGGCTGAAAATAAAACACAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1554

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2294 [new locus tag: SA_RS13155 ]
  • symbol: GntK
  • description: gluconokinase
  • length: 517
  • theoretical pI: 4.97775
  • theoretical MW: 58077
  • GRAVY: -0.109284

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Sugars gluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 1004.5)
    and 8 more
    Metabolism Energy metabolism Sugars xylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 332.1)
    Metabolism Energy metabolism Other glycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 205.3)
    Metabolism Energy metabolism Sugars L-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 119)
    FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 95.9)
    Metabolism Energy metabolism Sugars ribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 63)
    rhamnulokinase (TIGR02627; EC 2.7.1.5; HMM-score: 60.7)
    putative CoA-substrate-specific enzyme activase (TIGR00241; HMM-score: 21)
    benzoyl-CoA reductase, subunit A (TIGR02259; EC 1.3.7.8; HMM-score: 11)
  • TheSEED  :
    • Gluconokinase (EC 2.7.1.12)
    Carbohydrates Monosaccharides D-gluconate and ketogluconates metabolism  Gluconokinase (EC 2.7.1.12)
  • PFAM:
    Actin_ATPase (CL0108) FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 238.7)
    and 3 more
    FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 108.2)
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 24.9)
    no clan defined DDE_Tnp_IS66_C; IS66 C-terminal element (PF13817; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003802
    • TAT(Tat/SPI): 0.000095
    • LIPO(Sec/SPII): 0.000766
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKYMIGVDIGTTSTKSVLYDENGAFIMKHQIGYDLHTPNVDVSEENPDELFDAVLMTIKYIMRESKVNQDDIKFVSFSAQMHSLIAMDQQHQRLTNNITWADNRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLVKIFWMKHEWQDVFQRTAKFADIKTYIFYHLFDTYIIDYSMASATGMFNLETLDWDVGALELLGISKEMFPELVPTTYVMKGMKERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTDYKGRIFCYVLTEDHYVIGGPVNNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIAKRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYTVYLALIEVMNETPNMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGLKAVGDIEDFSIVSSMVGATNNHTPFEENVAVYQELVSIFINLSRSLTENYEQIADFQRQHMAENKTQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA regulon, GntR (repression) regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 
    GntR(TF)important in Gluconate utilization; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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