From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1818 [new locus tag: SA_RS10440 ]
  • pan locus tag?: SAUPAN005244000
  • symbol: SA1818
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1818 [new locus tag: SA_RS10440 ]
  • symbol: SA1818
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2059637..2060197
  • length: 561
  • essential: no DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAATAAAAAACTATTAACAAAAACATTGATAGCAAGTGCTTTAGTTTTAACAACAGTA
    GGTTCAGGTTTTCATTCTTCTTCAAATTATAATGGTATTAATAACGTTGCAAAAGCTGAG
    CAAACGACAGATAACGCATTGTGGAAAAATGTAAGAGACGCTTTAAAAGACGCGAATATT
    ATCGATAAAACAGATAATGAAAATGTCAAGGTTACGTATAAAATAGAAAATGGTGGAGAA
    AATACCATAGAAGGAACAGCTAATTTAGAAAATATTAGTACTTCAAACAATCCTAAAATA
    AACCCTCAAAATGTTACAAAAATTAATATAACTAGAAAAAATTCGAACTACCCTAATATT
    GATGCTAATAATACATGGAAAAAATTAACAGAAAAATTGAAAGAAAAAAATATAGTGAAC
    AACGGCGACAATGTTTCAATCTTAAGTACAGACCCTAAAGATGAGACTGTATTCGGTAAA
    GTAGGAGAAGATAAATCAAACGTAAGCAATAGATACATCAATCCTAAAGATATAAATGAA
    ATTCAAATCACTAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    561


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1818 [new locus tag: SA_RS10440 ]
  • symbol: SA1818
  • description: hypothetical protein
  • length: 186
  • theoretical pI: 9.74077
  • theoretical MW: 20605.8
  • GRAVY: -0.82957

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions putative phage terminase, small subunit, P27 family (TIGR01558; HMM-score: 20.1)
    and 1 more
    Genetic information processing Transcription Transcription factors transcription elongation factor GreA (TIGR01462; HMM-score: 15.2)
  • TheSEED  :
    • Secreted protein Ear, superantigen-encoding pathogenicity islands SaPI
    Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Staphylococcal pathogenicity islands SaPI  Secreted protein Ear, superantigen-encoding pathogenicity islands SaPI
  • PFAM:
    no clan defined DUF4888; Domain of unknown function (DUF4888) (PF16229; HMM-score: 66.3)
    and 6 more
    DUF7599; Domain of unknown function (DUF7599) (PF24538; HMM-score: 21.3)
    PK_C; Pyruvate kinase, alpha/beta domain (PF02887; HMM-score: 16.1)
    LepB_GAP_C; LepB GAP domain C-terminal subdomain (PF18227; HMM-score: 14.6)
    WYL (CL0654) WYL_2; WYL_2, Sm-like SH3 beta-barrel fold (PF10902; HMM-score: 13.5)
    no clan defined Terminase_4; Phage terminase, small subunit (PF05119; HMM-score: 12.9)
    P-loop_NTPase (CL0023) nSTAND_NTPase4; Novel STAND NTPase 4 (PF24406; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.023
    • Extracellular Score: 0.9768
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 39 aa
    • SP(Sec/SPI): 0.783596
    • TAT(Tat/SPI): 0.003552
    • LIPO(Sec/SPII): 0.182171
    • Cleavage Site: CS pos: 39-40. AKA-EQ. Pr: 0.4078
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKKLLTKTLIASALVLTTVGSGFHSSSNYNGINNVAKAEQTTDNALWKNVRDALKDANIIDKTDNENVKVTYKIENGGENTIEGTANLENISTSNNPKINPQNVTKINITRKNSNYPNIDANNTWKKLTEKLKEKNIVNNGDNVSILSTDPKDETVFGKVGEDKSNVSNRYINPKDINEIQITKK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise 

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]


⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA1818&oldid=128075"