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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA1872 [new locus tag: SA_RS10765 ]
- pan locus tag?: SAUPAN005336000
- symbol: rsbU
- pan gene symbol?: rsbU
- synonym:
- product: sigmaB regulation protein RsbU
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA1872 [new locus tag: SA_RS10765 ]
- symbol: rsbU
- product: sigmaB regulation protein RsbU
- replicon: chromosome
- strand: -
- coordinates: 2119821..2120822
- length: 1002
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1124771 NCBI
- RefSeq: NP_375175 NCBI
- BioCyc: see SA_RS10765
- MicrobesOnline: 104201 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961GTGGAAGAATTTAAGCAACATTATAAGGGTTTAATTGATGAAAGTTTAACGTGCCAAGAT
AAAGTAGAATTGATAAAAAAGTGTGAGAAATACACTGACGAAGTGATTCGTAAGGACGTC
TTGCCTGAAGACATTGTCGATATTCACAAAAACTATATATTGACGTTAAACTTAACGCGT
GAAGATGTGTTCAAGACATTAGATGTCTTACAAGAAATCGTTAAAGGCTTTGGTTATAGT
TATCGAGATTATCAAAGATTGGTAGATAAACTTCAAGTTCACGATAAAGAGATAGACTTA
GCTTCTAGCTTACAACAAACAATGCTTAAAACAGATATTCCACAATTTGATAGTATTCAA
ATTGGCGTTATTTCAGTGGCAGCACAAAAAGTAAGTGGAGATTATTTTAATTTAATTGAC
CATAACGATGGCACAATGAGCTTTGCTGTTGCAGATGTCATTGGGAAAGGTATACCAGCT
GCTTTAGCAATGAGTATGATAAAGTTTGGCATGGATTCTTATGGACACTCACAATTACCG
AGTGATGGCTTAAAACGTTTAAATCGTGTTGTTGAAAAGAATATTAATCAAAATATGTTC
GTCACAATGTTTTATGGTTTATATGAAGAAATGAACCATTTATTGTATTGTAGTTCAGCT
GGTCATGAGCCTGGATATATTTATCGCGCTGAAAAAGAAGAATTTGAAGAAATTTCAGTT
AGAGGTAGAGTGTTAGGAATCAGTTCACAAACACGATATCAACAACAAGAAATTCCTATA
TACCTTGATGATTTAATTATCATTTTAACGGATGGTGTGACTGAAGCTAGAAATAGTGAA
GGTACCTTTATAGATAAACAAAAACTTTTAGAATATATTAAAAAACATAAACATATGCAC
CCACAAGATATTGTTCAAATTATCTATGAAGCAATTTTAAAGCTTCAAAACCCAAATAAA
AAAGATGATATGACTATTTTGATTATAAAAAGAGTAAATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA1872 [new locus tag: SA_RS10765 ]
- symbol: RsbU
- description: sigmaB regulation protein RsbU
- length: 333
- theoretical pI: 5.44262
- theoretical MW: 38425.9
- GRAVY: -0.334835
⊟Function[edit | edit source]
- TIGRFAM: Cellular processes Sporulation and germination stage II sporulation protein E (TIGR02865; EC 3.1.3.16; HMM-score: 52.8)
- TheSEED :
- Serine phosphatase RsbU, regulator of sigma subunit
- PFAM: PP2C (CL0238) SpoIIE; Stage II sporulation protein E (SpoIIE) (PF07228; HMM-score: 164.2)and 1 moreKaiA_RbsU (CL0637) RsbU_N; Phosphoserine phosphatase RsbU, N-terminal domain (PF08673; HMM-score: 52.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002671
- TAT(Tat/SPI): 0.000403
- LIPO(Sec/SPII): 0.001476
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPEDIVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLASSLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPAALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCSSAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEARNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: sigB < rsbW < rsbV < rsbU
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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