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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1872 [new locus tag: SA_RS10765 ]
  • pan locus tag?: SAUPAN005336000
  • symbol: rsbU
  • pan gene symbol?: rsbU
  • synonym:
  • product: sigmaB regulation protein RsbU

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA1872 [new locus tag: SA_RS10765 ]
  • symbol: rsbU
  • product: sigmaB regulation protein RsbU
  • replicon: chromosome
  • strand: -
  • coordinates: 2119821..2120822
  • length: 1002
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGGAAGAATTTAAGCAACATTATAAGGGTTTAATTGATGAAAGTTTAACGTGCCAAGAT
    AAAGTAGAATTGATAAAAAAGTGTGAGAAATACACTGACGAAGTGATTCGTAAGGACGTC
    TTGCCTGAAGACATTGTCGATATTCACAAAAACTATATATTGACGTTAAACTTAACGCGT
    GAAGATGTGTTCAAGACATTAGATGTCTTACAAGAAATCGTTAAAGGCTTTGGTTATAGT
    TATCGAGATTATCAAAGATTGGTAGATAAACTTCAAGTTCACGATAAAGAGATAGACTTA
    GCTTCTAGCTTACAACAAACAATGCTTAAAACAGATATTCCACAATTTGATAGTATTCAA
    ATTGGCGTTATTTCAGTGGCAGCACAAAAAGTAAGTGGAGATTATTTTAATTTAATTGAC
    CATAACGATGGCACAATGAGCTTTGCTGTTGCAGATGTCATTGGGAAAGGTATACCAGCT
    GCTTTAGCAATGAGTATGATAAAGTTTGGCATGGATTCTTATGGACACTCACAATTACCG
    AGTGATGGCTTAAAACGTTTAAATCGTGTTGTTGAAAAGAATATTAATCAAAATATGTTC
    GTCACAATGTTTTATGGTTTATATGAAGAAATGAACCATTTATTGTATTGTAGTTCAGCT
    GGTCATGAGCCTGGATATATTTATCGCGCTGAAAAAGAAGAATTTGAAGAAATTTCAGTT
    AGAGGTAGAGTGTTAGGAATCAGTTCACAAACACGATATCAACAACAAGAAATTCCTATA
    TACCTTGATGATTTAATTATCATTTTAACGGATGGTGTGACTGAAGCTAGAAATAGTGAA
    GGTACCTTTATAGATAAACAAAAACTTTTAGAATATATTAAAAAACATAAACATATGCAC
    CCACAAGATATTGTTCAAATTATCTATGAAGCAATTTTAAAGCTTCAAAACCCAAATAAA
    AAAGATGATATGACTATTTTGATTATAAAAAGAGTAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1002

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA1872 [new locus tag: SA_RS10765 ]
  • symbol: RsbU
  • description: sigmaB regulation protein RsbU
  • length: 333
  • theoretical pI: 5.44262
  • theoretical MW: 38425.9
  • GRAVY: -0.334835

Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination stage II sporulation protein E (TIGR02865; EC 3.1.3.16; HMM-score: 52.8)
  • TheSEED  :
    • Serine phosphatase RsbU, regulator of sigma subunit
    Stress Response Stress Response - no subcategory SigmaB stress responce regulation  Serine phosphatase RsbU, regulator of sigma subunit
  • PFAM:
    PP2C (CL0238) SpoIIE; Stage II sporulation protein E (SpoIIE) (PF07228; HMM-score: 164.2)
    and 1 more
    KaiA_RbsU (CL0637) RsbU_N; Phosphoserine phosphatase RsbU, N-terminal domain (PF08673; HMM-score: 52.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002671
    • TAT(Tat/SPI): 0.000403
    • LIPO(Sec/SPII): 0.001476
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPEDIVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLASSLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPAALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCSSAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEARNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]