From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1921 [new locus tag: SA_RS11045 ]
  • pan locus tag?: SAUPAN005410000
  • symbol: tdk
  • pan gene symbol?: tdk
  • synonym:
  • product: thymidine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1921 [new locus tag: SA_RS11045 ]
  • symbol: tdk
  • product: thymidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2169392..2169991
  • length: 600
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGTATGAAACTTACCATTCCGGTTGGATAGAATGTATTACAGGTAGTATGTTTAGCGGT
    AAATCCGAAGAACTAATAAGACGTCTAAGAAGGGGTATATACGCTAAACAAAAAGTCGTT
    GTATTTAAACCGGCGATTGATGATCGATATCATAAAGAAAAAGTGGTCTCTCATAATGGT
    AATGCCATTGAAGCGATTAATATTTCTAAAGCAAGTGAAATTATGACGCACAACTTAACT
    AATGTTGATGTTATAGGAATAGACGAAGTGCAATTTTTTGACGATGAAATTGTAAGTATA
    GTAGAAAAACTATCAGCAGATGGACACCGTGTTATTGTTGCTGGATTAGATATGGATTTT
    AGGGGCGAACCGTTCGAACCAATGCCTAAATTGATGGCTGTCAGTGAACAAGTAACGAAA
    CTTCAAGCAGTTTGTGCTGTTTGTGGTTCTTCATCTAGTAGAACGCAACGATTAATAAAT
    GGTAAACCAGCTAAAATCGATGATCCGATTATCTTAGTAGGTGCCAATGAAAGTTATGAG
    CCACGTTGTCGCGCGCACCATATCGTAGCACCTAGTGATAATAATAAGGAGGAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1921 [new locus tag: SA_RS11045 ]
  • symbol: Tdk
  • description: thymidine kinase
  • length: 199
  • theoretical pI: 6.15911
  • theoretical MW: 22213.2
  • GRAVY: -0.264322

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.21?  ExPASy
    Thymidine kinase ATP + thymidine = ADP + thymidine 5'-phosphate
  • TIGRFAM:
    Cellular processes Cellular processes Chemotaxis and motility flagellar motor switch protein FliG (TIGR00207; HMM-score: 13)
  • TheSEED  :
    • Thymidine kinase (EC 2.7.1.21)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  Thymidine kinase (EC 2.7.1.21)
    and 1 more
    Nucleosides and Nucleotides Pyrimidines pyrimidine conversions  Thymidine kinase (EC 2.7.1.21)
  • PFAM:
    P-loop_NTPase (CL0023) TK; Thymidine kinase (PF00265; HMM-score: 197)
    and 1 more
    AAA_19; AAA domain (PF13245; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004685
    • TAT(Tat/SPI): 0.000712
    • LIPO(Sec/SPII): 0.00219
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKPAIDDRYHKEKVVSHNGNAIEAINISKASEIMTHNLTNVDVIGIDEVQFFDDEIVSIVEKLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLINGKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA1921&oldid=85516"