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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1957 [new locus tag: SA_RS11235 ]
  • pan locus tag?: SAUPAN005471000
  • symbol: SA1957
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1957 [new locus tag: SA_RS11235 ]
  • symbol: SA1957
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2208144..2209001
  • length: 858
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGATAACGTAAAAGCAATATTTTTGGACATGGATGGAACAATTTTACATGAAAATAAT
    CAAGCATCAACGTATACGAAAGATGTCATTAATCAATTGAGAGAGAAAGGATATAAAGTA
    TTTTTGGCAACAGGACGTTCGCATTCTGAAATACATCAACTTGTACCTCAAGATTTTGCG
    GTTAATGGCATCATTAGTTCAAATGGAACAATTGGAGAAGTAGATGGAGAAATTATCTTC
    AAGCATGGTTTATCATTGGCTCAAGTGCAACAAATTACTAATTTAGCTAAGCGCCAACAA
    ATTTATTATGAGGTATTTCCTTTTGAAGGTAATAGAGTTTCTTTAAAAGAAGATGAAACA
    TGGATGCGAGATATGATTCGTAGTCAAGATCCTATTAATGGCGTAAGTCATAGTGAATGG
    TCTTCAAGACAAGATGCGCTTGCTGGTAAGATAGATTGGGTAACTAAGTTTCCTGAAGGT
    GAATATTCAAAAATTTATCTATTCAGTTCTAATTTAGAAAAAATAACAGCATTTAGAGAT
    GAATTAAAGCAAAATCATGTGCAACTACAGATTAGTGTTTCAAATTCATCAAGATTTAAT
    GCGGAAACAATGGCTTATCAAACTGATAAAGGTACAGGCATTAAAGAAATGATTGCACAT
    TTTGGTATTCATCAAGAAGAAACGTTAGTTATTGGAGATAGCGACAATGATAGAGCAATG
    TTTGAATTTGGTCATTATACAGTTGCTATGAAAAATGCACGCCCTGAAATCCAAGCATTA
    ACTTCAGATGTAACGGCATACACGAATGAAGAGGATGGCGCAGCAAAATATTTAGCAAAG
    CATTTTTTAGCTGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    858

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1957 [new locus tag: SA_RS11235 ]
  • symbol: SA1957
  • description: hypothetical protein
  • length: 285
  • theoretical pI: 5.07078
  • theoretical MW: 32282.8
  • GRAVY: -0.489825

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 171.3)
    and 19 more
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 88.7)
    sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 88.4)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 83.2)
    mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 50.1)
    mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 46.9)
    sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 38.9)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 37.2)
    Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 32.4)
    Unknown function General mannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 30.9)
    sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 26.5)
    Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 24.3)
    HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 21.2)
    histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 19.2)
    phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 18.7)
    HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 18.5)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 18.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 17.5)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 13.9)
    phosphonatase-like hydrolase (TIGR03351; HMM-score: 12.7)
  • TheSEED  :
    • Hydrolase, alpha/beta fold family
  • PFAM:
    HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 178.4)
    and 8 more
    S6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 57.5)
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 38)
    HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 22.1)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 21.3)
    Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 20.4)
    Acid_phosphat_B; HAD superfamily, subfamily IIIB (Acid phosphatase) (PF03767; HMM-score: 17.3)
    GT-B (CL0113) Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 14.6)
    Cache (CL0165) sCache_like; Single Cache-like (PF16736; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00549
    • TAT(Tat/SPI): 0.00027
    • LIPO(Sec/SPII): 0.000604
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLATGRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIYYEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEGEYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMIAHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAKYLAKHFLAD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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