From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02396
  • pan locus tag?: SAUPAN005471000
  • symbol: SAOUHSC_02396
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02396
  • symbol: SAOUHSC_02396
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2215122..2215979
  • length: 858
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGATAACGTAAAAGCAATATTTTTGGACATGGATGGAACAATTTTACATGAAAATAAT
    CAAGCATCAACGTATACGAAAGATGTCATTAATCAATTGAGAGAGAAAGGATATAAAGTA
    TTTTTGGCAACAGGACGTTCGCATTCTGAAATACATCAACTTGTACCTCAAGATTTTGCG
    GTTAATGGCATCATTAGTTCAAATGGAACAATTGGAGAAGTAGATGGAGAAATTATCTTC
    AAGCATGGTTTATCATTGGCTCAAGTGCAACAAATTACTAATTTAGCTAAGCGCCAACAA
    ATTTATTATGAGGTATTTCCTTTTGAAGGTAATAGAGTTTCTTTAAAAGAAGATGAAACA
    TGGATGCGAGATATGATTCGTAGTCAAGATCCTATTAATGGCGTAAGTCATAGTGAATGG
    TCTTCAAGACAAGATGCGCTTGCTGGTAAGATAGATTGGGTAACTAAGTTTCCTGAAGGT
    GAATATTCAAAAATTTATCTATTCAGTTCTAATTTAGAAAAAATAACAGCATTTAGAGAT
    GAATTAAAGCAAAATCATGTGCAACTACAGATTAGTGTTTCAAATTCATCAAGATTTAAT
    GCGGAAACAATGGCTTATCAAACTGATAAAGGTACAGGCATTAAAGAAATGATTGCACAT
    TTTGGTATTCATCAAGAAGAAACGTTAGTTATTGGAGATAGCGACAATGATAGAGCAATG
    TTTGAATTTGGTCATTATACAGTTGCTATGAAAAATGCACGCCCTGAAATCCAAGCATTA
    ACTTCAGATGTAACGGCATACACGAATGAAGAGGATGGCGCAGCAAAATATTTAGCAGAG
    CATTTTTTAGCTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    858

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02396
  • symbol: SAOUHSC_02396
  • description: hypothetical protein
  • length: 285
  • theoretical pI: 4.8743
  • theoretical MW: 32297.8
  • GRAVY: -0.488421

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 171.3)
    and 19 more
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 88.7)
    sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 88.6)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 83.2)
    mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 50.3)
    mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 46.9)
    sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 38.9)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 37.2)
    Metabolism Amino acid biosynthesis Serine family phosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 32.4)
    Unknown function General mannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 30.9)
    sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 26.6)
    Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 24.3)
    HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 21.3)
    histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 19.2)
    phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 18.7)
    HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 18.5)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IB (TIGR01490; HMM-score: 18.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 18.1)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 13.8)
    phosphonatase-like hydrolase (TIGR03351; HMM-score: 12.7)
  • TheSEED  :
    • Hydrolase (HAD superfamily)
  • PFAM:
    HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 178.4)
    and 8 more
    S6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 57.5)
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 38.1)
    HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 22.1)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 21.3)
    Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 20.4)
    Acid_phosphat_B; HAD superfamily, subfamily IIIB (Acid phosphatase) (PF03767; HMM-score: 17.3)
    GT-B (CL0113) Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 14.6)
    Cache (CL0165) sCache_like; Single Cache-like (PF16736; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00549
    • TAT(Tat/SPI): 0.00027
    • LIPO(Sec/SPII): 0.000604
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDNVKAIFLDMDGTILHENNQASTYTKDVINQLREKGYKVFLATGRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQITNLAKRQQIYYEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEGEYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMIAHFGIHQEETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAKYLAEHFLAE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_02396&oldid=60139"