From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2053 [new locus tag: SA_RS11800 ]
  • pan locus tag?: SAUPAN005709000
  • symbol: SA2053
  • pan gene symbol?: glcU
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA2053 [new locus tag: SA_RS11800 ]
  • symbol: SA2053
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2314403..2315266
  • length: 864
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    TTGCAATTTCTTGATTTCTTAATCGCACTTTTACCTGCTTTATTCTGGGGAAGTGTCGTT
    CTTATTAATGTGTTCGTCGGCGGTGGACCTTACAACCAAATTCGTGGTACGACGCTAGGC
    GCACTCATTGTTGGATTAGGTTTACTTATTACTGGTTTCGCAAAATTCAATAATCCTACT
    GTAATTATTGTCGGTCTTATTTCTGGTGCATTATGGGCGTTTGGACAAGCGAATCAGCTT
    AAATCTATTAGTTTAATCGGTGTATCAAATACTATGCCAGTTTCTACAGGTATGCAATTA
    GTTGGTACAACATTATTCAGCGTTATCTTTTTAGGTGAATGGTCTTCAATGACTCAAATT
    ATCTTTGGTTTAATCGCCATGATATTATTAGTTACTGGTGTAGCACTTACTTCACTTAAA
    GCCAAAAATGAACGTCAATCAGATAATCCTGAATTTAAAAAAGCAATGGGTATTTTAATT
    GTATCTACAGTTGGATATGTAGGTTTCGTTGTACTTGGTGACATCTTTGGTGTTGGTGGA
    ACTGATGCATTGTTCTTCCAATCTGTCGGTATGGCAATTGGTGGCTTTATCCTATCCATG
    AATCATAAAACATCACTTAAATCAACAGCACTTAATCTATTGCCAGGTGTGATTTGGGGA
    ATTGGTAACTTGTTCATGTTCTATTCTCAACCAAAAGTTGGTGTAGCTACAAGTTTCTCA
    TTATCACAGTTACTTGTTATCGTTTCAACATTAGGTGGTATTTTCATTTTAGGAGAAAGA
    AAAGATCGTCGTCAGATGACGGGTATTTGGGCAGGTATTATTATTATCGTGATAGCTGCT
    ATAATTCTAGGTAATTTGAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    864

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA2053 [new locus tag: SA_RS11800 ]
  • symbol: SA2053
  • description: hypothetical protein
  • length: 287
  • theoretical pI: 10.52
  • theoretical MW: 30393.1
  • GRAVY: 0.93554

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids RhaT L-rhamnose-proton symporter family protein (TIGR00776; HMM-score: 317)
  • TheSEED  :
    • Glucose:proton symporter GlcU
  • PFAM:
    DMT (CL0184) Sugar_transport; Sugar transport protein (PF06800; HMM-score: 329.9)
    and 7 more
    EamA; EamA-like transporter family (PF00892; HMM-score: 24.5)
    Ureide_permease; Ureide permease (PF07168; HMM-score: 21.3)
    GPCR_A (CL0192) Ceramidase; Ceramidase (PF05875; HMM-score: 10.5)
    PepSY_TM-like (CL0490) PepSY_TM_like_2; Putative PepSY_TM-like (PF16357; HMM-score: 9.8)
    DMT (CL0184) TMEM144; Transmembrane family, TMEM144 of transporters (PF07857; HMM-score: 8.2)
    no clan defined PRA1; PRA1 family protein (PF03208; HMM-score: 7.6)
    Thioredoxin (CL0172) OST3_OST6; OST3 / OST6 family, transporter family (PF04756; HMM-score: 5.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016323
    • TAT(Tat/SPI): 0.000692
    • LIPO(Sec/SPII): 0.019264
  • predicted transmembrane helices (TMHMM): 9

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIVGLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVGTTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILIVSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVIWGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIIIVIAAIILGNLK

Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]