From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2093 [new locus tag: SA_RS12025 ]
  • pan locus tag?: SAUPAN005767000
  • symbol: ssaA
  • pan gene symbol?: ssaA
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA2093 [new locus tag: SA_RS12025 ]
  • symbol: ssaA
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2352596..2353399
  • length: 804
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAGAAAATCGCTACAGCTACTATCGCAACTGCAGGATTCGCTACAATCGCAATTGCA
    TCAGGAAATCAAGCTCATGCTTCTGAGCAAGATAACTACGGTTATAATCCAAACGACCCA
    ACATCATATAGCTATACTTACACTATTGATGCACAAGGTAACTACCATTACACATGGAAA
    GGTAACTGGCATCCAAGTCAATTAAACCAAGATAATGGCTACTACAGCTATTACTACTAC
    AATGGCTACAATAACTACAACAATTACAACAATGGTTATAGCTACAATAATTACAGCCGT
    TACAACAACTACTCAAATAATAATCAATCATATAACTACAATAACTATAATAGTTACAAC
    ACAAACAGCTACCGTACTGGTGGTTTAGGTGCAAGCTACAGCACTTCAAGCAACAATGTT
    CAAGTAACTACAACTATGGCTCCATCATCAAATGGCCGTTCAATCTCAAGTGGTTATACT
    TCAGGACGTAACTTATACACTTCTGGTCAATGTACATACTACGTATTTGATCGTGTAGGT
    GGTAAAATCGGTTCAACTTGGGGCAATGCAAGTAACTGGGCTAACGCAGCTGCAAGAGCT
    GGTTACACAGTGAACAATACACCAAAAGCTGGTGCAATTATGCAAACAACTCAAGGTGCA
    TACGGTCACGTTGCATACGTTGAAAGTGTTAACAGCAATGGTTCAGTAAGAGTTTCAGAA
    ATGAACTATGGTTATGGCCCAGGTGTTGTAACTTCACGTACAATCTCAGCTAGCCAAGCT
    GCTGGTTATAACTTCATTCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    804

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA2093 [new locus tag: SA_RS12025 ]
  • symbol: SsaA
  • description: hypothetical protein
  • length: 267
  • theoretical pI: 9.09862
  • theoretical MW: 29327
  • GRAVY: -0.846816

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Secretory antigen SsaA
  • PFAM:
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 54.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: NQAHASEQ
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.998381
    • TAT(Tat/SPI): 0.000739
    • LIPO(Sec/SPII): 0.000657
    • Cleavage Site: CS pos: 27-28. AHA-SE. Pr: 0.9903
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYSYTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNNYNNGYSYNNYSRYNNYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSGYTSGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQTTQGAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]