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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2181 [new locus tag: SA_RS12515 ]
  • pan locus tag?: SAUPAN005924000
  • symbol: SA2181
  • pan gene symbol?: nreA
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2181 [new locus tag: SA_RS12515 ]
  • symbol: SA2181
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2449454..2449906
  • length: 453
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    TTGACACCAGAAGCAATAATTGAAGATCGTAGATTTCAAGAGACATTAGATAAAATACGC
    AAAGAAGAGGGTTATGATTTTGCGGCAATCGCTTTCTATGAATCTAACAAACCATCATCT
    CCAATAAAATGGCACTATGTTTCAGGTAATAAAAATAATCGATTTAAATTGATTATTTTA
    AGAAAAGGTCGTGGATTAGCTGGAACTGTTATGAAAACAGGAAAGCGTATGGTAATAGCC
    AATGTTGGTTTAGCTTTAGGGCCTGAAGAAAAGATTGACTATCCAATTTTATTGAGCGAA
    TCATTAACAGCAGTTTTAGCAGTGCCGCTTTGGTATAAGAATCAAGTGTATGGCGTACTA
    TTATTTGGTCAAAGAGATGGTCGTCCTTTACCAAAAATATTTGATAATGATGATATTCAA
    CGTAAGTTTGGTATTTTCAATGATGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    453

⊟Protein[edit | edit source]

Protein Data Bank: 4PAU

⊟General[edit | edit source]

  • locus tag: SA2181 [new locus tag: SA_RS12515 ]
  • symbol: SA2181
  • description: hypothetical protein
  • length: 150
  • theoretical pI: 9.92029
  • theoretical MW: 17149.7
  • GRAVY: -0.381333

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Central intermediary metabolism Nitrogen fixation Nif-specific regulatory protein (TIGR01817; HMM-score: 11.6)
    Signal transduction Regulatory functions DNA interactions Nif-specific regulatory protein (TIGR01817; HMM-score: 11.6)
  • TheSEED  :
    • Oxygen-sensing, dissimilatory nitrate and nitrite reduction regulatory protein NreA
  • PFAM:
    GAF (CL0161) GAF_2; GAF domain (PF13185; HMM-score: 34.5)
    and 2 more
    GAF; GAF domain (PF01590; HMM-score: 26.6)
    GAF_3; GAF domain (PF13492; HMM-score: 20.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008644
    • TAT(Tat/SPI): 0.003867
    • LIPO(Sec/SPII): 0.002183
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKWHYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMVIANVGLALGPEEKIDYPILLSESLTAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, NreC* (activation) regulon
    Rex*(TF)important in Energy metabolism; RegPrecise 
    NreC*(TF)important in Nitrate and nitrite respiration; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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