From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2201
  • pan locus tag?: SAUPAN005947000
  • symbol: SA2201
  • pan gene symbol?: tcyB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2201
  • symbol: SA2201
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2470101..2470595
  • length: 495
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGATAGTACAACTATTTATCATATTTTATGGTATTCCAGAATTAGGTAGATTATTAACA
    AATGACGCTGACAACCAATGGACATTGGCACCTGTAGTGGCTGCTATTATTGGTTTATCA
    TTAAATGTAGGTGCGTATGCTTCGGAAATTATTCGTGGCGGTATTATTTCTATACCGAAA
    GGACAAACAGAAGCTGCATATTCCATCGGTATGACGTATGGTCAAACGATACAACGTATC
    ATTTTACCGCAGGCAATTCGAGTGTCGATTCCTGCACTAGGTAATACATTTTTAAGTTTA
    ATCAAAGATACATCATTATTAGGATTTATTTTAGTGGCTGAAATGTTTAGAAAAGCTCAA
    GAAGTTGCGTCTACAACATATGAATATTTAACAATTTATGTGTTAGTTGCGCTAATGTAC
    TGGGTGGTATGCTTTATTATTTCAATTATCCAAGGTATCTATGAATCTTATATTGAAAGA
    GGGTATCGCTCATGA
    60
    120
    180
    240
    300
    360
    420
    480
    495

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2201
  • symbol: SA2201
  • description: hypothetical protein
  • length: 164
  • theoretical pI: 5.0682
  • theoretical MW: 18181.3
  • GRAVY: 0.677439

⊟Function[edit | edit source]

  • TIGRFAM:
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 119.7)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 106)
    and 4 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 32.8)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 22.3)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 21.1)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 15.3)
  • TheSEED  :
    • amino acid ABC transporter, permease protein
    Sulfur Metabolism Organic sulfur assimilation L-Cystine Uptake and Metabolism  L-Cystine ABC transporter, permease protein TcyB
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 71.8)
    and 1 more
    no clan defined NEMP; NEMP family (PF10225; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.186698
    • TAT(Tat/SPI): 0.007076
    • LIPO(Sec/SPII): 0.002631
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIVQLFIIFYGIPELGRLLTNDADNQWTLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYGQTIQRIILPQAIRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVVCFIISIIQGIYESYIERGYRS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CymR* (repression) regulon
    CymR*(TF)important in Cysteine metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA2201&oldid=95426"