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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2381 [new locus tag: SA_RS13635 ]
  • pan locus tag?: SAUPAN006279000
  • symbol: SA2381
  • pan gene symbol?: sasK
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2381 [new locus tag: SA_RS13635 ]
  • symbol: SA2381
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2669869..2670504
  • length: 636
  • essential: no DEG

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAAAAAGTTATAAGTTTATTTGTTGCAATTTTATCAGTGTTGATTTTTACGGATAAT
    GCGAACGCCGAAAATAACAAACCTGAAGGTGTGAATGAAAACAGCAATTTAATACCAGTT
    AGACAACCGGATGCAAATTATCCAGGGCCAGTTAGTGACATAGCACGTGTGAAAAAACTT
    GATAATTTAATAGATGGCACGAAAGATCCAAATGTTATCAAGCAGTTGAAAGATGATAAT
    GAGGTGCCGAATAATGCGGTAAAGGCAAAAGATTCTAAAATAGTATCCGACTGCAGACAT
    TTACTAAAACATCCAGATAATTTAATAGATGGCACGAAAGATCCAAATGTTATTAATCAG
    TTGAAAAACGCAAATGGAACATCGAATAATAGTGCTGTAAATCCAAGTATTAAAGCAAAT
    GATATTCACACAAAAAGTAATAAACAAATGGCTAAACAACAAAGTATTCATTTGGATAGC
    AAGAGCAAGTCAGTACATAAGCAACAATTGAAAGAATTGCCAAAAACAGGAGTAAACAAC
    GAAAGTAATACAGCAATAATGGCTTTATTTATTGGTTTAATATTGCTTGTAGTAAGTTTA
    GTGACAAGATTTATAAAATTAAATAGCCATCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    636

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2381 [new locus tag: SA_RS13635 ]
  • symbol: SA2381
  • description: hypothetical protein
  • length: 211
  • theoretical pI: 10.1625
  • theoretical MW: 23177.3
  • GRAVY: -0.475355

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Other LPXTG cell wall anchor domain (TIGR01167; HMM-score: 17.3)
  • TheSEED  :
    • Predicted cell-wall-anchored protein SasK (LPXTG motif)
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Predicted cell-wall-anchored protein SasK (LPXTG motif)
  • PFAM:
    no clan defined Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 26)
    and 6 more
    eIF3_N; eIF3 subunit 6 N terminal domain (PF09440; HMM-score: 15.5)
    DUF5383; Family of unknown function (DUF5383) (PF17355; HMM-score: 15.4)
    Glycophorin_A; Glycophorin A (PF01102; HMM-score: 14.6)
    DUF3899; Domain of unknown function (DUF3899) (PF13038; HMM-score: 12.6)
    DUF3188; Protein of unknown function (DUF3188) (PF11384; HMM-score: 12.2)
    Peptidase_CA (CL0125) Menin; Menin (PF05053; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: found LPxTG motif :LPKTG
  • SignalP: Signal peptide SP(Sec/SPI) length 23 aa
    • SP(Sec/SPI): 0.995633
    • TAT(Tat/SPI): 0.000316
    • LIPO(Sec/SPII): 0.002572
    • Cleavage Site: CS pos: 23-24. ANA-EN. Pr: 0.9834
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKVISLFVAILSVLIFTDNANAENNKPEGVNENSNLIPVRQPDANYPGPVSDIARVKKLDNLIDGTKDPNVIKQLKDDNEVPNNAVKAKDSKIVSDCRHLLKHPDNLIDGTKDPNVINQLKNANGTSNNSAVNPSIKANDIHTKSNKQMAKQQSIHLDSKSKSVHKQQLKELPKTGVNNESNTAIMALFIGLILLVVSLVTRFIKLNSHQ

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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