PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2388 [new locus tag: SA_RS13670 ]
• pan locus tag^?: SAUPAN006290000
• symbol: tnpA
• pan gene symbol^?: tnpA
• synonym:
• product: transposition regulatory protein tnpA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2388 [new locus tag: SA_RS13670 ]
• symbol: tnpA
• product: transposition regulatory protein tnpA
• replicon: chromosome
• strand: -
• coordinates: 2676598..2677683
• length: 1086
• essential: no DEG

⊟Accession numbers

• Gene ID: 1125317 NCBI
• RefSeq: NP_375714 NCBI
• BioCyc: see SA_RS13670
• MicrobesOnline: 104740 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  
  GTGAAGGTTCAAAGGATAGAAGTGGAGAATAAGCCGTATCCATTGTATTTATTACTAGAT
  AAAGAATACCAGCTAATAGAACCAGTAATGAAATTTATTAAATACTTAGATAATACTGGT
  AAGTCTCCTAATACCATTAAGGCATACTGCTATCATTTAAAGTTGCTGTACGAGTTCATG
  GAACAGAGAGGTGTTATTCTTAATGATATTAACTTTGAGTTGTTAGCAGACTTCGTAGGT
  TGGTTGAGATATCCTTCAGCATCAAATGTAATTGATCTTCAGTCAAAAAAAGCCATAAGA
  GAAGAAACGACAGTGAATACAATTTTAAATGTAGTTATGAGTTTTCTTGATTATTTAAGT
  AGATTAGGAGAATTTAAATCAATTGATGTATTTAAACAAGCAAAGGGAAGAAATTTCAAA
  GGATTTTTACATCATGTTAATAAGGGTAGATACCAAAAGAATGTCTTAAAGTTAAGGGTT
  AAAAAGAAACAGATAAGAACATTGAGATCAAAGGAAGTTAAGCAAATTATTGATGCTTGT
  CATACGAAAAGAGATAAATTAATTTTAATGCTTATGTATGAGGGTGGTTTAAGAATCGGT
  GAAGTGTTATCGCTTAGGCTTGAAGATATTGTCACTTGGGACAATCAAATCCATTTAACA
  CCTAGAGATGTTAATGTTAATGAAGCTTATATTAAATTAAGGAAGGAAAGAACAATACAT
  GTGAGTAAAGAACTTATGTCACTTTATACAGATTACTTGATATATGAGTATAGTGAGGAA
  TTGGAGCATGATTATGTTTTTATTTCCTTAAAAGAAGGCTATTTTGGGAAACCACTAAAG
  TACCAAAGTGTTCTTGATCTAGTTAGAAGAATAGTTAAAAGGACTGGAATAGAATTTACA
  TCACATATGCTTCGCCACACTCACGCAACGCAGCTAATTAGGGAAGGATGGGATGTTGCG
  TTCGTTCAAAAGAGATTAGGTCACGCACATGTTCAGACAACGTTAAATACCTATGTTCAT
  CTTTCAGATCAGGATATGAAAAATGAGTTTAATAAATACCTCGAGAGAAAGGAGCATAAG
  AAATGA
  60
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  1086
  

⊟Protein

⊟General

• locus tag: SA2388 [new locus tag: SA_RS13670 ]
• symbol: TnpA
• description: transposition regulatory protein tnpA
• length: 361
• theoretical pI: 9.89064
• theoretical MW: 42940.7
• GRAVY: -0.441828

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerC (TIGR02224; HMM-score: 108.3)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair tyrosine recombinase XerD (TIGR02225; HMM-score: 105.8)
  and 2 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair integron integrase (TIGR02249; HMM-score: 58.1)
  Genetic information processing Mobile and extrachromosomal element functions Other integron integrase (TIGR02249; HMM-score: 58.1)
• TheSEED  :
  • Transposase A from transposon Tn554
• PFAM:
  DNA-mend (CL0382) Phage_integrase; Phage integrase family (PF00589; HMM-score: 154.1)
  and 3 more
  l-integrase_N (CL0469) Phage_int_SAM_1; Phage integrase, N-terminal SAM-like domain (PF02899; HMM-score: 31.1)
  Phage_int_SAM_4; Phage integrase, N-terminal SAM-like domain (PF13495; HMM-score: 23.8)
  Phage_int_SAM_5; Phage integrase SAM-like domain (PF13102; HMM-score: 20.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002319
  • TAT(Tat/SPI): 0.000069
  • LIPO(Sec/SPII): 0.000316
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15928181 NCBI
• RefSeq: NP_375714 NCBI
• UniProt: P0A051 UniProt

⊟Protein sequence

• MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPNTIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREETTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRVKKKQIRTLRSKEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIHLTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFGKPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTTLNTYVHLSDQDMKNEFNKYLERKEHKK

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: tnpC < tnpB < tnpA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

T Ito, Y Katayama, K Hiramatsu
Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315.
Antimicrob Agents Chemother: 1999, 43(6);1449-58
[PubMed:10348769] [WorldCat.org] [DOI] (P p)