PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL0168 [new locus tag: SACOL_RS00855 ]
• pan locus tag^?: SAUPAN001023000
• symbol: argJ
• pan gene symbol^?: argJ
• synonym:
• product: bifunctional ornithine acetyltransferase/N-acetylglutamate synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL0168 [new locus tag: SACOL_RS00855 ]
• symbol: argJ
• product: bifunctional ornithine acetyltransferase/N-acetylglutamate synthase
• replicon: chromosome
• strand: -
• coordinates: 192647..193888
• length: 1242
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3236042 NCBI
• RefSeq: YP_185067 NCBI
• BioCyc: see SACOL_RS00855
• MicrobesOnline: 911645 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAACATCAAGAAACGACATCACAACAATATAACTTTTCAATTATTAAACATGGCGAT
  ATCAGTACACCTCAAGGCTTCACGGCTGGTGGTATGCACATCGGTTTACGCGCTAACAAA
  AAAGACTTTGGGTGGATTTACTCATCGTCTTTGGCAAGTGCAGCTGCCGTATATACTTTA
  AATCAGTTTAAAGCTGCACCACTTATTGTCACTGAAGACACTTTACAAAAGTCTAAAGGA
  AAATTACAAGCACTTGTTGTTAATTCAGCTAATGCAAATTCTTGTACCGGTCAACAAGGC
  ATAGATGATGCACGACAAACACAAACATGGGTTGCTCAACAACTTCAAATACCATCTGAG
  CATGTTGCTGTTGCTTCAACTGGGGTCATTGGTGAATATTTGCCTATGGATAAAATTAAG
  ACTGGGACCGAACATATTAAGGATGCTAATTTTGCAACGCCAGGTGCGTTTAACGAGGCA
  ATTTTAACAACTGATACCTGTACAAAACATATCGCTGTATCACTAAAAATCGATGGTAAA
  ACCGTTACAATTGGTGGTAGCACCAAAGGTTCAGGTATGATTCACCCAAATATGGCTACC
  ATGCTTGCTTTTATAACAACCGATGCATCGATTGAATCGAATACACTTCATCAATTATTA
  AAATCTTCGACTGACCATACATTTAATATGATTACTGTTGATGGCGATACAAGTACAAAT
  GACATGGTATTAGTCATGGCAAATCACCAAGTTGAACACCAAATACTTAGTCAAGACCAT
  CCACAATGGGAAACATTTGTTGATGCATTCAATTTTGTCTGTACATTTTTAGCTAAAGCT
  ATAGCCAGAGATGGCGAAGGCGCAACAAAGTTAATCAGCGTCAATGTGTCAGGCGCTAAA
  TCAATATCAGATGCAAGAAAAATCGGTAAAACTATCGTAAGTTCAAATCTAGTAAAATCA
  GCTATTTTTGGCGAAGATGCCAATTTTGGTCGAATCATTACAGCTATTGGCTACAGCGGA
  TGTGAAATTGATCCTAACTGCACATATGTTCAACTGAACCAAATACCTGTCGTTGATAAA
  GGTATGGCTGTACTATTTGATGAGCAAGCTATGTCGAATACATTAACTCATGAAAATGTC
  ACAATTGACGTTCAGCTTGGTTTAGGTAACGCTGCAGCGACTGCATACGGTTGTGATTTA
  TCCTATGATTATGTGCGTATCAACGCATCATATCGAACATAA
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  1242
  

⊟Protein

⊟General

• locus tag: SACOL0168 [new locus tag: SACOL_RS00855 ]
• symbol: ArgJ
• description: bifunctional ornithine acetyltransferase/N-acetylglutamate synthase
• length: 413
• theoretical pI: 6.18088
• theoretical MW: 44399.8
• GRAVY: -0.108232

⊟Function

• reaction:
  EC 2.3.1.1?  ExPASy
  Amino-acid N-acetyltransferase Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate
  EC 2.3.1.35?  ExPASy
  Glutamate N-acetyltransferase N²-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate
• TIGRFAM:
  Metabolism Amino acid biosynthesis Glutamate family glutamate N-acetyltransferase/amino-acid acetyltransferase (TIGR00120; EC 2.3.1.1,2.3.1.35; HMM-score: 494.5)
• TheSEED  :
  • Glutamate N-acetyltransferase (EC 2.3.1.35)
  • N-acetylglutamate synthase (EC 2.3.1.1)
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Glutamate N-acetyltransferase (EC 2.3.1.35)
  and 1 more
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  N-acetylglutamate synthase (EC 2.3.1.1)
• PFAM:
  DmpA_ArgJ (CL0635) ArgJ; ArgJ family (PF01960; HMM-score: 481.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.67
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.027663
  • TAT(Tat/SPI): 0.010515
  • LIPO(Sec/SPII): 0.002982
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 57651184 NCBI
• RefSeq: YP_185067 NCBI
• UniProt: Q5HJJ0 UniProt

⊟Protein sequence

• MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFGWIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGIDDARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKTGTEHIKDANFATPGAFNEAILTTDTCTKHIAVSLKIDGKTVTIGGSTKGSGMIHPNMATMLAFITTDASIESNTLHQLLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILSQDHPQWETFVDAFNFVCTFLAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIITAIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHENVTIDVQLGLGNAAATAYGCDLSYDYVRINASYRT

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: argB < argJ < argC < rocD1

⊟Regulation

• regulator: ArgR/AhrC (repression) regulon

  ArgR/AhrC

  (TF)

  important in Arginine biosynthesis, Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications