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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0167 [new locus tag: SACOL_RS00850 ]
  • pan locus tag?: SAUPAN001021000
  • symbol: argB
  • pan gene symbol?: argB
  • synonym:
  • product: acetylglutamate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0167 [new locus tag: SACOL_RS00850 ]
  • symbol: argB
  • product: acetylglutamate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 191867..192631
  • length: 765
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAATTTATTGTCATTAAAATTGGTGGCAGTACACTTAGTGACATGCATCCATCAATT
    ATTAACAACATTAAGCATTTACGATCAAACAACATCTACCCCATTATCGTTCATGGCGGT
    GGCCCATTTATTAATGAAGCATTATCAAACCAGCAAATCGAGCCACACTTTGTTAATGGC
    CTAAGAGTGACTGATAAAGCAACCATGACCATTACTAAACACACGCTCATTGCAGACGTT
    AACACTGCATTAGTAGCTCAATTTAACCAGCACCAATGTTCTGCAATAGGCTTATGTGGT
    TTGGATGCACAGCTGTTTGAAATTACATCTTTTGATCAACAATATGGATATGTCGGTGTT
    CCGACCGCTTTAAATAAGGATGCTTTACAGTATTTATGTACTAAATTTGTACCTATCATC
    AATTCGATTGGTTTCAATAACCATGATGGAGAATTTTACAATATTAATGCTGACACGCTT
    GCCTATTTTATTGCATCATCATTAAAAGCGCCTATTTATGTATTAAGTAATATTGCAGGT
    GTACTCATCAATGATGTTGTTATACCTCAATTGCCATTAGTCGATATTCATCAATATATT
    GAACATGGTGATATTTATGGAGGTATGATTCCCAAAGTGCTAGATGCCAAAAATGCGATT
    GAAAATGGCTGTCCTAAAGTTATCATTGCATCAGGAAACAAGCCAAATATCATTGAATCT
    ATTTACAATAATGATTTTGTTGGCACAACAATCCTTAATTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    765

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0167 [new locus tag: SACOL_RS00850 ]
  • symbol: ArgB
  • description: acetylglutamate kinase
  • length: 254
  • theoretical pI: 6.21852
  • theoretical MW: 27738.7
  • GRAVY: 0.183858

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.2.8?  ExPASy
    Acetylglutamate kinase ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family acetylglutamate kinase (TIGR00761; EC 2.7.2.8; HMM-score: 284.1)
    and 4 more
    Metabolism Amino acid biosynthesis Glutamate family amino-acid N-acetyltransferase (TIGR01890; EC 2.3.1.1; HMM-score: 51.3)
    Metabolism Amino acid biosynthesis Glutamate family glutamate 5-kinase (TIGR01027; EC 2.7.2.11; HMM-score: 18.6)
    Metabolism Amino acid biosynthesis Glutamate family delta l-pyrroline-5-carboxylate synthetase (TIGR01092; HMM-score: 17.3)
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 14)
  • TheSEED  :
    • N-acetylglutamate kinase (EC 2.7.2.8)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Acetylglutamate kinase (EC 2.7.2.8)
  • PFAM:
    no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 137.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011465
    • TAT(Tat/SPI): 0.000175
    • LIPO(Sec/SPII): 0.001681
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFINEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLDAQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTLAYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKNAIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: ArgR (repression) regulon
    ArgR(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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