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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00147
  • pan locus tag?: SAUPAN001021000
  • symbol: SAOUHSC_00147
  • pan gene symbol?: argB
  • synonym:
  • product: acetylglutamate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00147
  • symbol: SAOUHSC_00147
  • product: acetylglutamate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 157954..158718
  • length: 765
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAATTTATTGTCATTAAAATTGGTGGCAGTACACTTAGTGACATGCATCCATCAATT
    ATTAACAACATTAAGCATTTACGATCAAACAACATCTACCCCATTATCGTTCATGGCGGT
    GGCCCATTTATTAATGAAGCATTATCAAACCAGCAAATCGAGCCACACTTTGTTAATGGC
    CTAAGAGTGACTGATAAAGCAACCATGACCATTACTAAACACACGCTCATTGCAGACGTT
    AACACTGCATTAGTAGCTCAATTTAACCAGCACCAATGTTCTGCAATAGGCTTATGTGGT
    TTGGATGCACAGCTGTTTGAAATTACATCTTTTGATCAACAATATGGATATGTCGGTGTT
    CCGACCGCTTTAAATAAGGATGCTTTACAGTATTTATGTACTAAATTTGTACCTATCATC
    AATTCGATTGGTTTCAATAACCATGATGGAGAATTTTACAATATTAATGCTGACACGCTT
    GCCTATTTTATTGCATCATCATTAAAAGCGCCTATTTATGTATTAAGTAATATTGCAGGT
    GTACTCATCAATGATGTTGTTATACCTCAATTGCCATTAGTCGATATTCATCAATATATT
    GAACATGGTGATATTTATGGAGGTATGATTCCCAAAGTGCTAGATGCCAAAAATGCGATT
    GAAAATGGCTGTCCTAAAGTTATCATTGCATCAGGAAACAAGCCAAATATCATTGAATCT
    ATTTACAATAATGATTTTGTTGGCACAACAATCCTTAATTCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    765

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00147
  • symbol: SAOUHSC_00147
  • description: acetylglutamate kinase
  • length: 254
  • theoretical pI: 6.21852
  • theoretical MW: 27738.7
  • GRAVY: 0.183858

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.2.8?  ExPASy
    Acetylglutamate kinase ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family acetylglutamate kinase (TIGR00761; EC 2.7.2.8; HMM-score: 284.1)
    and 4 more
    Metabolism Amino acid biosynthesis Glutamate family amino-acid N-acetyltransferase (TIGR01890; EC 2.3.1.1; HMM-score: 51.3)
    Metabolism Amino acid biosynthesis Glutamate family glutamate 5-kinase (TIGR01027; EC 2.7.2.11; HMM-score: 18.6)
    Metabolism Amino acid biosynthesis Glutamate family delta l-pyrroline-5-carboxylate synthetase (TIGR01092; HMM-score: 17.3)
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 14)
  • TheSEED  :
    • N-acetylglutamate kinase (EC 2.7.2.8)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Acetylglutamate kinase (EC 2.7.2.8)
  • PFAM:
    no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 137.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011465
    • TAT(Tat/SPI): 0.000175
    • LIPO(Sec/SPII): 0.001681
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFINEALSNQQIEPHFVNGLRVTDKATMTITKHTLIADVNTALVAQFNQHQCSAIGLCGLDAQLFEITSFDQQYGYVGVPTALNKDALQYLCTKFVPIINSIGFNNHDGEFYNINADTLAYFIASSLKAPIYVLSNIAGVLINDVVIPQLPLVDIHQYIEHGDIYGGMIPKVLDAKNAIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: ArgR* (repression) regulon
    ArgR*(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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