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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0179 [new locus tag: SACOL_RS00910 ]
  • pan locus tag?: SAUPAN001036000
  • symbol: SACOL0179
  • pan gene symbol?: murR
  • synonym:
  • product: RpiR family phosphosugar-binding transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0179 [new locus tag: SACOL_RS00910 ]
  • symbol: SACOL0179
  • product: RpiR family phosphosugar-binding transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 206627..207502
  • length: 876
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGACAAATATTTTATATCGCATTGATAAGCAGTTGAGTGATTTTACGAAGACAGAAAAG
    ATAATCGCTGATTACATTTTAAAGAATCCACATAAAATCATTGATATGACTGTGAATGAT
    TTGGCAGATGTTACGAATGTTAGTACAGCATCAATTGTTAGATTTAGTCGGAAAATGACA
    CATCAAGGTTTTCAAGAGCTAAAGATTGCGATATCTCGATACTTACCCGAAGATATTGCA
    ACCAATCCACATTTAGAATTGATTGAAAATGAATCTGTAGAAACTTTGAAAAATAAAATG
    ATTGCTAGAGCAACGAATACGATGCGATTTGTAGCTACTAATATTATGGATGCGCAAATT
    GATGCAATTTGTGATGTGTTGAAAAATGCCAGGACAATATTTTTATTTGGATTTGGCGCA
    TCGAGTTTGACTATTGGTGATCTTTTTCAAAAGTTATCTCGTATTGGCTTAAATGTCAGG
    TTATTACATGAAACGCATTTACTTGTGTCAACATTTGCGACGCATGATGATAGAGATTGC
    ATGATTTTTGTGACGAATCAAGGTAGTCATAGTGAATTGCAGTCAATTGCACAGGTGGCC
    ACACATTACAGTATTCCCATCATAACTATATCTAGTACAGCTAATAATCCAGTGGCTCAA
    ATTGCAGACTATGCATTGATTTATGGCAGAACTGATGAAAATGAAATGCGTATGGCGGCT
    ACAACGTCACTATTTGCACAGTTATTCACGGTAGATATATTGTACTATCGATTTGTAGCA
    TTAAATTATCATGCGATTCTAGATTGTATAACCCAATCGAAAATGGCACTTGATAATTAC
    AGGAAGCATCTTGCGACGATAGATTTTAAACATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    876

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0179 [new locus tag: SACOL_RS00910 ]
  • symbol: SACOL0179
  • description: RpiR family phosphosugar-binding transcriptional regulator
  • length: 291
  • theoretical pI: 6.91264
  • theoretical MW: 32986.7
  • GRAVY: 0.00274914

⊟Function[edit | edit source]

  • TIGRFAM:
    6-phospho 3-hexuloisomerase (TIGR03127; EC 5.3.-.-; HMM-score: 41.6)
    and 1 more
    Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 29.6)
  • TheSEED  :
    • Sialic acid utilization regulator, RpiR family
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  Sialic acid utilization regulator, RpiR family
  • PFAM:
    HTH (CL0123) HTH_6; Helix-turn-helix domain, rpiR family (PF01418; HMM-score: 80)
    and 6 more
    SIS (CL0067) SIS; SIS domain (PF01380; HMM-score: 53.3)
    SIS_2; SIS domain (PF13580; HMM-score: 16.8)
    HTH (CL0123) MarR; MarR family (PF01047; HMM-score: 15.4)
    MarR_2; MarR family (PF12802; HMM-score: 15.3)
    no clan defined DUF2325; Uncharacterized protein conserved in bacteria (DUF2325) (PF10087; HMM-score: 13.9)
    HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: N-acetylmuramate-6-phosphate
  • genes regulated by MurR*, TF important in N-acetylmuramate utilizationRegPrecise
    repression
    mupG* > murQ > murP* > murR*
    transcription unit transferred from N315 data RegPrecise

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002902
    • TAT(Tat/SPI): 0.000097
    • LIPO(Sec/SPII): 0.000253
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADVTNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENESVETLKNKMIARATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVRLLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPVAQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMALDNYRKHLATIDFKH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: MurR* (repression) regulon, CcpA regulon
    MurR*(TF)important in N-acetylmuramate utilization; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CcpA(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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