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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0217 [new locus tag: SACOL_RS01110 ]
  • pan locus tag?: SAUPAN001102000
  • symbol: SACOL0217
  • pan gene symbol?: nikA
  • synonym: opp5A
  • product: ABC transporter substrate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0217 [new locus tag: SACOL_RS01110 ]
  • symbol: SACOL0217
  • product: ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 258147..259622
  • length: 1476
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAGTTTAAAAGACTAGCAACTATATTTTCAGCAGTATTAGTGTTATCAGGATGTGGC
    TCAATGCATTCATCTGGCAAAGACTTAAATATTTCGTTACCGTTAAAAACAAAGTCTATT
    GCACCTTATGAAACAGATGTACCAGTTAAGATTGGTGCTGCAGAATCATTATTTAAAACA
    AATGATCAAGGAAAAATAGAAAAAGCATTAGTGAAATCGTATCATCAACCAAATGATACA
    ACGTTAGATATAGAATTAAAAGATAATATTAAATTTCAAAACGGTCAAAAATTGACTGCA
    GAAAAAGTGAAATCTAGCCTTGAAAATAGCATGAAAAAAAGCGACTTGGTCAAATATTCA
    TTACCAATATCATCAATTACCGCTAAAGGTCAAAAACTGACAATTAAAACCAACTCCGCT
    TACCCTGAACTTGTATCTGAATTAGCTAATCCTTTTATGGCAATTTATGATACAGATGCT
    AAATCAGATGTTAATCAAACTCCTGTTGGTACAGGCCCTTACCAAATAAAAGATTATAAG
    CAATCTCGAAAAATATCATTGTCGAATTTTAAGGACTATTGGCAAGGTAAACCGAAACTT
    GATCATATTACTGTGACGTACCAAGAAGACGGCAATAATCGCGTCAGAAATTTAGAATCT
    CAAAAAGATGATTTAATAACTGATGTCCCAGTTAATAAAGTTCAAGACATAGAAAATAAT
    CAAAATTTAAAAGTGTCAAAAGAATCTGGATTTAGAACTTCTTTACTTATGTATAATCAT
    ACTAATAAAAAAATGACTAAATCCGTTCGTGAAGCATTAGATCATATCATTGATAGACAA
    GGTATTGCAGATCATATTTATCAAGGTTATGCGAAACCTGCAACGAGTCCATTTAATGAT
    AAAATTCCATATATTAAAGAACCTAAGTTAACCAAACAAAATATCGAACAAGCAAAAATG
    TTATTAGCTAAAGATGGTTATACAAAAGAACACCCTTTAAAAATTAAGTTAATTACGTAT
    GACGGTCGTCCAGAGCTATCGAAAATTGCGCAAGTATTACAATCAGATGCTAAAAAAGCA
    AATATTGAAATCGACATTAAAAGTGTTGATGACATAGAAGGTTACTTAAAAGACCGTTCT
    GCATGGGATGCAACGATGTATAGTTTCGGAACAATTCCTCGTGGAGATACAGGTTATTTC
    TTTAATCAAGCATATAAAAAAGATGGTGCTATTAATAAAGGAGACTACAATAATAGCAAC
    GTCGATGATTTAATTAATCAATTGAATCATACTGTAGATGTTAAGGAACGTCACAATATT
    TCTAATGATATTATTAAATTATCAAGTCGAGATGTACCAAACAGTTATATTGCTTACAAC
    GATCAAATAGTCGCTGCTAACTCTAAGGTTAAAAATTATAAAGTAACACCTGAAGGTATC
    TATTTAATCGATTATCGAACAACGATTGAACGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1476

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0217 [new locus tag: SACOL_RS01110 ]
  • symbol: SACOL0217
  • description: ABC transporter substrate-binding protein
  • length: 491
  • theoretical pI: 9.5255
  • theoretical MW: 55430.4
  • GRAVY: -0.670061

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, nickel/metallophore periplasmic binding protein (TIGR02294; HMM-score: 117.6)
    and 2 more
    Metabolism Transport and binding proteins Unknown substrate ABC transporter substrate binding protein, KPN_01854 family (TIGR04028; HMM-score: 42.2)
    glycosyltransferase, SP_1767 family (TIGR03728; HMM-score: 17.3)
  • TheSEED  :
    • Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1)
    Membrane Transport ABC transporters ABC transporter oligopeptide (TC 3.A.1.5.1)  Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1)
    and 1 more
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Sex pheromones in Enterococcus faecalis and other Firmicutes  Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1)
  • PFAM:
    PBP (CL0177) SBP_bac_5; Bacterial extracellular solute-binding proteins, family 5 Middle (PF00496; HMM-score: 223.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 9.2
    • Extracellular Score: 0.78
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: VLSGCGS
  • SignalP: Signal peptide LIPO(Sec/SPII) length 18 aa
    • SP(Sec/SPI): 0.002815
    • TAT(Tat/SPI): 0.000092
    • LIPO(Sec/SPII): 0.996788
    • Cleavage Site: CS pos: 18-19. LSG-CG. Pr: 0.9975
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEKVKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDAKSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLKIKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Lipoprotein [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Aurelia Hiron, Elise Borezée-Durant, Jean-Christophe Piard, Vincent Juillard
Only one of four oligopeptide transport systems mediates nitrogen nutrition in Staphylococcus aureus.
J Bacteriol: 2007, 189(14);5119-29
[PubMed:17496096] [WorldCat.org] [DOI] (P p)Aurelia Hiron, Brunella Posteraro, Marie Carrière, Laetitia Remy, Cécile Delporte, Marilena La Sorda, Maurizio Sanguinetti, Vincent Juillard, Elise Borezée-Durant
A nickel ABC-transporter of Staphylococcus aureus is involved in urinary tract infection.
Mol Microbiol: 2010, 77(5);1246-60
[PubMed:20662775] [WorldCat.org] [DOI] (I p)

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