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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0289 [new locus tag: SACOL_RS01465 ]
  • pan locus tag?: SAUPAN001212000
  • symbol: SACOL0289
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0289 [new locus tag: SACOL_RS01465 ]
  • symbol: SACOL0289
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 327492..327983
  • length: 492
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGACTTTCGAAGAAAAATTAAGTGAAATGTATAGCGAGATTGCGAATAAGATTATTAGC
    ATGATACCGGTAGAGTGGGAAAAAGTATATGCAATGGCATATATCGATGAAGAATGTGGA
    GAAGTGTTTTACAATTATACTGAACCAAGCAGTGATGAATTATTTTACTATACAAGTGTG
    ATAAAGAAGTATAATTTATTGAAATCAAGCTTTATGGATTCAGTATATGAGTTGCATGAT
    CAATTTGAGGAACTAAGAGAAGTATTTATAGAAGAAGGTCTTGAACCATGGACATCATGC
    GAATTTGATTTTACAAAAGAAGATAAATTAAAAGTTTCATTTGATTATATTGATTGGATA
    AATACAGAGTTTGATCAATTAGGCCGTGAAAATTATTATATGTACAAAAAATTTGGTGTT
    TTACCAGAAACGGAATATGAAATTAATAAAGTTAAAGAAATCGAGCAATATATTAAAGAG
    CAAGAAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    492

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0289 [new locus tag: SACOL_RS01465 ]
  • symbol: SACOL0289
  • description: hypothetical protein
  • length: 163
  • theoretical pI: 3.95498
  • theoretical MW: 19864.1
  • GRAVY: -0.61227

⊟Function[edit | edit source]

  • TIGRFAM:
    conserved hypothetical protein (TIGR01741; HMM-score: 275.7)
  • TheSEED  :
    • Repetitive hypothetical protein near ESAT cluster, SA0282 homolog
    Membrane Transport Protein secretion system, Type VII ESAT-6 proteins secretion system in Firmicutes  Repetitive hypothetical protein near ESAT cluster, SA0282 homolog
  • PFAM:
    no clan defined DUF600; Protein of unknown function, DUF600 (PF04634; HMM-score: 146.7)
    and 1 more
    CBP4; CBP4 (PF07960; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015416
    • TAT(Tat/SPI): 0.000409
    • LIPO(Sec/SPII): 0.000888
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTFEEKLSEMYSEIANKIISMIPVEWEKVYAMAYIDEECGEVFYNYTEPSSDELFYYTSVIKKYNLLKSSFMDSVYELHDQFEELREVFIEEGLEPWTSCEFDFTKEDKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPETEYEINKVKEIEQYIKEQEE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell: 41 [2]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  2. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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