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NCBI: 10-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0310 [new locus tag: SACOL_RS01565 ]
  • pan locus tag?: SAUPAN001237000
  • symbol: SACOL0310
  • pan gene symbol?:
  • synonym:
  • product: nucleoside permease NupC

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0310 [new locus tag: SACOL_RS01565 ]
  • symbol: SACOL0310
  • product: nucleoside permease NupC
  • replicon: chromosome
  • strand: +
  • coordinates: 344220..345440
  • length: 1221
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATATTTTATTCGCTATCACAGGGATAGCATTTGCACTATTTGTTGCGTTTTTATTC
    AGTTTTGATCGTAAAAAAATAGACTTCAAAAAGACGTTAATAATGATATTTATTCAAGTG
    TTGATCGTGTTATTTATGATGAACACAACGATTGGTTTGACAATTTTAACTGCACTAGGT
    TCATTTTTTGAAGGGCTAATAAATATTAGTAAAGCAGGCATAAATTTTGTTTTTGGAGAT
    ATACAAAATAAAAATGGCTTTACGTTCTTTTTAAACGTATTACTGCCATTAGTTTTTATT
    TCTGTATTAATAGGCATCTTTAATTATATTAAGGTATTACCATTTATTATCAAATATGTA
    GGTATCGCTATTAATAAAATAACTAGAATGGGGCGCTTAGAAAGTTATTTTGCTATTTCA
    ACAGCAATGTTTGGGCAACCAGAAGTATATTTAACAATAAAAGATATTATTCCAAGATTA
    TCTAGAGCGAAATTATATACAATTGCGACGTCTGGTATGAGTGCTGTTAGTATGGCAATG
    CTAGGTTCATATATGCAGATGATTGAACCCAAGTTCGTAGTTACAGCAGTAATGTTAAAT
    ATTTTTAGTGCGCTTATCATCGCCAGTGTAATCAATCCCTATAAATCTGATGATACTGAT
    GTTGAAATTGATAACTTAACGAAATCCACAGAAACTAAAACATTGAATGGAAAAACAGGA
    AAACCTAAGAAAGTTGCCTTTTTCCAAATGATTGGTGATAGTGCGATGGATGGGTTTAAA
    ATCGCTGTTGTAGTAGCCGTAATGTTGTTAGCATTTATTTCATTAATGGAAGCAATTAAT
    ATCATGTTTGGTAGTGTTGGTTTGAACTTTAAACAGCTTATTGGCTATGTGTTTGCACCA
    ATCGCATTCTTAATGGGGATTCCATGGAGCGAAGCTGTTCCAGCTGGCTCTTTAATGGCG
    ACTAAATTAATTACAAATGAGTTTGTAGCAATGCTTGATTTTAAAAATGTCCTGGGTGAT
    GTATCAGCTCGAACACAAGGTATCATTTCAGTTTACTTAGTAAGCTTCGCTAATTTTGGT
    ACGGTTGGTATCATCGTAGGTTCAATTAAAGGCATTAGTGATAAACAAGGAGAAAAAGTT
    GCATCCTTTGCAATGAGGTTGCTACTTGGTTCAACTCTAGCTTCAATCATTTCAGGATCA
    ATCATTGGCTTAGTATTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1221

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL0310 [new locus tag: SACOL_RS01565 ]
  • symbol: SACOL0310
  • description: nucleoside permease NupC
  • length: 406
  • theoretical pI: 10.113
  • theoretical MW: 44220.8
  • GRAVY: 0.862562

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside transporter, NupC family (TIGR00804; HMM-score: 353.6)
  • TheSEED  :
    • Probable pyrimidine nucleoside transport protein associated with pseudouridine catabolism
    Nucleosides and Nucleotides Nucleosides and Nucleotides - no subcategory Pseudouridine Metabolism  Probable pyrimidine nucleoside transport protein associated with pseudouridine catabolism
  • PFAM:
    no clan defined Nucleos_tra2_C; Na+ dependent nucleoside transporter C-terminus (PF07662; HMM-score: 195.7)
    and 1 more
    Nucleos_tra2_N; Na+ dependent nucleoside transporter N-terminus (PF01773; HMM-score: 56.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005572
    • TAT(Tat/SPI): 0.000222
    • LIPO(Sec/SPII): 0.038552
  • predicted transmembrane helices (TMHMM): 8

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MNILFAITGIAFALFVAFLFSFDRKKIDFKKTLIMIFIQVLIVLFMMNTTIGLTILTALGSFFEGLINISKAGINFVFGDIQNKNGFTFFLNVLLPLVFISVLIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRLSRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDDTDVEIDNLTKSTETKTLNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLMEAINIMFGSVGLNFKQLIGYVFAPIAFLMGIPWSEAVPAGSLMATKLITNEFVAMLDFKNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGSTLASIISGSIIGLVL

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]