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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0407 [new locus tag: SACOL_RS02050 ]
  • pan locus tag?: SAUPAN001872000
  • symbol: glpT
  • pan gene symbol?: glpT
  • synonym:
  • product: glycerol-3-phosphate transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0407 [new locus tag: SACOL_RS02050 ]
  • symbol: glpT
  • product: glycerol-3-phosphate transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 413356..414714
  • length: 1359
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAATTTTCTTAAACCTGCAAAGCATATTAAGCCTTTGCCAGAAAATCAGATAGATGAT
    ACCTATAAACGATTACGTCTCCAAGTATTTCTTGGTATTTTCATCGGTTACGCTGGGTAC
    TATTTATTACGTAAAAACTTTTCGTTAGCGATGCCGGCATTGCAAGAGCAAGGTTTTACA
    AAAGCGGAACTAGGTTTTGCACTTTCTGCTGTTTCCATCGCATATGGATTTAGTAAGTTC
    TTTATGGGTACTGTAAGTGATCGGAGCAATGCTCGGATATTCTTAGTTCTTGGATTAGCA
    CTCACTGCTATCGTCAATTTGTTAATGGGATTTGTACCGTTCTTTACATCAGGTATCGGT
    ATTATGTTTGTCCTATTATTCTTAAATGGATGGTTTCAAGGTATGGGCTGGCCACCTTCA
    GGCCGTGTTCTCGTTCACTGGTTTAGTGTAAGTGAACGCGGAAGTAAGACTGCCCTTTGG
    AACGTTGCGCATAATGTTGGTGGAGGTATTATGGCACCTATTGCTGCTTGGGGTATTACA
    ACAACTGCATTTATCAACTTTGGTTATTTAAAAGGTTTCGAAGGTGTATTCATTTACCCT
    GCACTCTTAGCACTTATCATTGCCGCAATTTCATACGTATTGATTAGAGACACACCTCAA
    TCTCAAGGTTTACCTCCAATCGAAATTTATAAAAATGACTTTGCTACAAGCGATAAGAAA
    ACATTAGAAACAGAATTAACTACAAAAGAAATTTTATTTAAATATGTACTGAACAATAAA
    TGGGTATGGGCAATTGCCTTTGCAAATATATTTGTTTATTTCGTGCGTTATGGTGTACTT
    GATTGGGCGCCAGTCTACTTAAGTGAAGAAAAACATTTCGACTTAAAAGCATCAGGTTGG
    GCATACTTCTTATACGAATGGGCTGGAATTCCTGGTACATTATTATGTGGTTACATTTCT
    GATAAATTATTCAAAGGTCGTCGTGGACCTGCAGGTTTCTTCTTTATGTTAGGTGTCACA
    GTATTTGTATTAATTTATTGGTTAAATCCTCCAGGCAATGCTTGGTTAGACAATGTCTCA
    TTAATTGCCATTGGTTTCTTAATATATGGACCAGTTATGTTAATTGGTTTACAAGCATTA
    GATTATGTACCTAAAAAAGCAGCTGGCACAGCAGCTGGATTAACAGGATTATTTGGTTAT
    CTGTTTGGTGCTGTAATGGCCAACATCGTCTTAGGTGCTGTAGTTGATAAATTCGGATGG
    GATGTCGGTTTTATTTTATTAACAGCAATTAGTGTGTTTGCAATGTTGAGCTTTATCCTC
    ACTTGGAATAAAGTAGGACAAGAAACCGTTCATCATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1359

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0407 [new locus tag: SACOL_RS02050 ]
  • symbol: GlpT
  • description: glycerol-3-phosphate transporter
  • length: 452
  • theoretical pI: 9.59874
  • theoretical MW: 50167.7
  • GRAVY: 0.568363

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Other glycerol-3-phosphate transporter (TIGR00712; HMM-score: 666.5)
    and 10 more
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids phosphoglycerate transporter family protein (TIGR00881; HMM-score: 467.2)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-galactonate transporter (TIGR00893; HMM-score: 128)
    Metabolism Transport and binding proteins Anions Na(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 56.7)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 46.2)
    Metabolism Transport and binding proteins Unknown substrate MFS transporter, metabolite:H+ symporter (MHS) family protein (TIGR00883; HMM-score: 44.4)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids oxalate/formate antiporter family transporter (TIGR00890; HMM-score: 26.5)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids MFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 22.1)
    multidrug resistance protein (TIGR00880; HMM-score: 16.2)
    Cellular processes Cellular processes Pathogenesis type III secretion protein, HrcV family (TIGR01399; HMM-score: 9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type III secretion protein, HrcV family (TIGR01399; HMM-score: 9)
  • TheSEED  :
    • Glycerol-3-phosphate transporter
    Carbohydrates Sugar alcohols Glycerol and Glycerol-3-phosphate Uptake and Utilization  Glycerol-3-phosphate transporter
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 162.4)
    and 1 more
    OATP; Organic Anion Transporter Polypeptide (OATP) family (PF03137; HMM-score: 9.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013134
    • TAT(Tat/SPI): 0.001879
    • LIPO(Sec/SPII): 0.00081
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLRKNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALTAIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALWNVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDTPQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVRYGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFFFMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAAGLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETVHH

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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