From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0639 [new locus tag: SACOL_RS03300 ]
  • pan locus tag?: SAUPAN002374000
  • symbol: SACOL0639
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0639 [new locus tag: SACOL_RS03300 ]
  • symbol: SACOL0639
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 671333..671674
  • length: 342
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    ATGAAAAATACATTCCTTATTTGTGATGAATGTCAGGCAGTCAATATAAGAACGTTACAA
    AAGAAGTTGGAAAAATTAGATCCCGATGCTGAAATCGTGATAGGTTGTCAATCTTATTGT
    GGACCTGGACGCCGAAAAACATTCACTTTTGTTAATAACCGCCCACTGGCTGCGCTTACT
    GAAGAAGAATTAATCGAAAAAGTTTCTCAACAATTAAAGAAACCACGTGATCCTGAAGAA
    GAAGAGCGTTTAAGAAAACGACATGAAGAACGTAAACGTCGTAAAGAAGAACAAGATAGA
    AAGCTTAAAGAAAAATTAGAAAAGCGAAAAGCACAACAATAA
    60
    120
    180
    240
    300
    342

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0639 [new locus tag: SACOL_RS03300 ]
  • symbol: SACOL0639
  • description: hypothetical protein
  • length: 113
  • theoretical pI: 9.89847
  • theoretical MW: 13535.5
  • GRAVY: -1.31239

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation glutamyl-tRNA(Gln) amidotransferase, subunit D (TIGR02153; EC 6.3.5.-; HMM-score: 8.9)
  • TheSEED  :
    • FIG01308914: hypothetical protein YwzC
  • PFAM:
    no clan defined DUF1450; Protein of unknown function (DUF1450) (PF07293; HMM-score: 31.7)
    and 2 more
    Zn_Beta_Ribbon (CL0167) DUF2072; Zn-ribbon containing protein (PF09845; HMM-score: 15.7)
    BCLiA (CL0551) APG6; Autophagy protein Apg6 (PF04111; HMM-score: 8.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.013811
    • TAT(Tat/SPI): 0.000256
    • LIPO(Sec/SPII): 0.010296
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGRRKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDPEEEERLRKRHEERKRRKEEQDRKLKEKLEKRKAQQ

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL0639&oldid=102257"