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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0726 [new locus tag: SACOL_RS03730 ]
  • pan locus tag?: SAUPAN002548000
  • symbol: sarX
  • pan gene symbol?: sarX
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0726 [new locus tag: SACOL_RS03730 ]
  • symbol: sarX
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 752661..753086
  • length: 426
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGGCTGAAAAATTTAAGATAATAATGACAGAAGCATTGTCTTTATATATTTGGGGGTGC
    AACATTTTGAATACTGAGAAATTAGAAACATTGCTTGGCTTCTATAAACAATATAAAGCA
    TTATCTGAATATATTGATAAAAAATATAAGTTGTCGCTAAATGATTTAGCAGTCTTAGAT
    TTAACGATGAAGCATTGCAAAGATGAAAAAGTACTTATGCAATCATTTTTAAAAACTGCA
    ATGGATGAGCTAGATTTAAGTAGGACAAAATTATTAGTTTCTATAAGAAGACTAATTGAA
    AAAGAAAGACTTAGTAAAGTTAGATCATCTAAAGATGAGCGTAAAATTTATATTTATTTA
    AATAATGATGATATATCTAAATTTAATGCTTTATTTGAAGATGTAGAACAATTTTTAAAT
    ATTTAA
    60
    120
    180
    240
    300
    360
    420
    426

⊟Protein[edit | edit source]

Protein Data Bank: 5HS5

⊟General[edit | edit source]

  • locus tag: SACOL0726 [new locus tag: SACOL_RS03730 ]
  • symbol: SarX
  • description: hypothetical protein
  • length: 141
  • theoretical pI: 8.62636
  • theoretical MW: 16717.5
  • GRAVY: -0.251064

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 108.2)
    and 1 more
    Genetic information processing Protein synthesis tRNA aminoacylation histidine--tRNA ligase (TIGR00442; EC 6.1.1.21; HMM-score: 10.8)
  • TheSEED  :
    • FIG01108210: hypothetical protein
  • PFAM:
    HTH (CL0123) RNA_pol_Rpc34; RNA polymerase Rpc34 subunit (PF05158; HMM-score: 18.1)
    and 1 more
    no clan defined HPS6; Hermansky-Pudlak syndrome 6 protein (PF15702; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009607
    • TAT(Tat/SPI): 0.000534
    • LIPO(Sec/SPII): 0.173694
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAEKFKIIMTEALSLYIWGCNILNTEKLETLLGFYKQYKALSEYIDKKYKLSLNDLAVLDLTMKHCKDEKVLMQSFLKTAMDELDLSRTKLLVSIRRLIEKERLSKVRSSKDERKIYIYLNNDDISKFNALFEDVEQFLNI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Adhar C Manna, Ambrose L Cheung
Expression of SarX, a negative regulator of agr and exoprotein synthesis, is activated by MgrA in Staphylococcus aureus.
J Bacteriol: 2006, 188(12);4288-99
[PubMed:16740935] [WorldCat.org] [DOI] (P p)

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