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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0921 [new locus tag: SACOL_RS04725 ]
- pan locus tag?: SAUPAN003015000
- symbol: SACOL0921
- pan gene symbol?: mpfB
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0921 [new locus tag: SACOL_RS04725 ]
- symbol: SACOL0921
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 927776..928816
- length: 1041
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3237744 NCBI
- RefSeq: YP_185792 NCBI
- BioCyc: see SACOL_RS04725
- MicrobesOnline: 912392 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1021GTGATCATTGCCATAATTATATTGATATTTATTTCGTTTTTCTTTTCAGGAAGCGAGACG
GCATTAACGGCTGCCAATAAAGCAAAATTTAAAACTGAAGCTGACAAAGGTGATAAAAAA
GCAAAAGGCATTGTAAAGTTACTTGAAAAACCAAGTGAGTTTATTACAACGATTCTAATT
GGGAATAATGTCGCGAATATTTTATTACCAACACTTGTTACAATTATGGCTTTACGTTGG
GGGATTAGCGTTGGTATTGCATCAGCTGTTTTAACAGTTGTTATCATTTTGATCTCCGAA
GTGATTCCCAAGTCTGTCGCTGCAACATTTCCAGATAAAATAACAAGGCTTGTATATCCA
ATTATTAATATTTGTGTCATTGTGTTCCGTCCTATCACATTACTTTTAAATAAGTTGACG
GACAGTATTAATCGAAGTTTATCTAAGGGCCAACCTCAAGAACATCAATTTTCAAAAGAA
GAATTTAAAACAATGTTAGCAATTGCTGGACATGAAGGTGCTTTAAATGAAATTGAGACG
AGTAGGTTGGAAGGTGTCATTAATTTTGAAAATTTAAAAGTAAAAGATGTAGATACAACA
CCTAGAATTAATGTGACGGCATTTGCTTCAAATGCGACATACGAAGAAGTTTATGAAACG
GTTATGAATAAGCCATACACTAGATATCCAGTGTACGAGGGAGATATTGATAACATTATT
GGGGTGTTTCATTCTAAATATCTGTTGGCTTGGAGTAATAAAAAAGAAAATCAAATTACA
AACTATTCAGCTAAGCCATTATTTGTGAATGAACACAATAAAGCTGAATGGGTATTACGT
AAGATGACTATTTCTAGAAAACATTTAGCAATTGTGTTGGACGAATTTGGTGGTACTGAA
GCGATAGTGTCACATGAAGACTTAATTGAAGAATTATTAGGTATGGAAATTGAAGATGAG
ATGGATAAAAAGGAAAAAGAAAAACTTTCTCAACAGCAAATTCAATTTCAACAACGGAAA
AATCGCAACGTATCTATATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0921 [new locus tag: SACOL_RS04725 ]
- symbol: SACOL0921
- description: hypothetical protein
- length: 346
- theoretical pI: 7.16655
- theoretical MW: 38986
- GRAVY: 0.000867052
⊟Function[edit | edit source]
- TIGRFAM: gliding motility-associated protein GldE (TIGR03520; HMM-score: 207)
- TheSEED :
- Hemolysins and related proteins containing CBS domains
- PFAM: no clan defined DUF21; Domain of unknown function DUF21 (PF01595; HMM-score: 157.1)and 2 moreCBS; CBS domain (PF00571; HMM-score: 30.5)MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 11.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 9.68
- Cellwall Score: 0.17
- Extracellular Score: 0.16
- Internal Helices: 2
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0
- Signal peptide possibility: 1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: Signal peptide SP(Sec/SPI) length 23 aa
- SP(Sec/SPI): 0.63781
- TAT(Tat/SPI): 0.008428
- LIPO(Sec/SPII): 0.154797
- Cleavage Site: CS pos: 23-24. ALT-AA. Pr: 0.2082
- predicted transmembrane helices (TMHMM): 4
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MIIAIIILIFISFFFSGSETALTAANKAKFKTEADKGDKKAKGIVKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVIPKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKEEFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVYETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAEWVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQIQFQQRKNRNVSI
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Integral membrane [1] [2]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
J Bacteriol: 2004, 186(13);4085-99
[PubMed:15205410] [WorldCat.org] [DOI] (P p)