From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00854
  • pan locus tag?: SAUPAN003015000
  • symbol: SAOUHSC_00854
  • pan gene symbol?: mpfB
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00854
  • symbol: SAOUHSC_00854
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 824165..825205
  • length: 1041
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    GTGATCATTGCCATAATTATATTGATATTTATTTCGTTTTTCTTTTCAGGAAGCGAGACG
    GCATTAACGGCTGCCAATAAAACAAAATTTAAAACTGAAGCTGACAAAGGTGATAAAAAA
    GCAAAAGGCATTGTAAAGTTACTTGAAAAACCAAGTGAGTTTATTACAACGATTCTAATT
    GGGAATAATGTCGCGAATATTTTATTACCAACACTTGTTACAATTATGGCTTTACGTTGG
    GGGATTAGCGTTGGTATTGCATCAGCTGTTTTAACAGTTGTTATCATTTTGATCTCCGAA
    GTGATTCCCAAGTCTGTCGCTGCAACATTTCCAGATAAAATAACAAGGCTTGTATATCCA
    ATTATTAATATTTGTGTCATTGTGTTCCGTCCTATCACATTACTTTTAAATAAGTTGACG
    GACAGTATTAATCGAAGTTTATCTAAGGGCCAACCTCAAGAACATCAATTTTCAAAAGAA
    GAATTTAAAACAATGTTAGCAATTGCTGGACATGAAGGTGCTTTAAATGAAATTGAGACG
    AGTAGGTTGGAAGGTGTCATTAATTTTGAAAATTTAAAAGTAAAAGATGTAGATACAACA
    CCTAGAATTAATGTGACGGCATTTGCTTCAAATGCGACATACGAAGAAGTTTATGAAACG
    GTTATGAATAAGCCATACACTAGATATCCAGTGTACGAGGGAGATATTGATAACATTATT
    GGGGTGTTTCATTCTAAATATCTGTTGGCTTGGAGTAATAAAAAAGAAAATCAAATTACA
    AACTATTCAGCTAAGCCATTATTTGTGAATGAACACAATAAAGCTGAATGGGTATTACGT
    AAGATGACTATTTCTAGAAAACATTTAGCAATTGTGTTGGACGAATTTGGTGGTACTGAA
    GCGATAGTGTCACATGAAGACTTAATTGAAGAATTATTAGGTATGGAAATTGAAGATGAG
    ATGGATAAAAAGGAAAAAGAAAAACTTTCTCAACAGCAAATTCAATTTCAACAACGGAAA
    AATCGCAACGTATCTATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1041

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00854
  • symbol: SAOUHSC_00854
  • description: hypothetical protein
  • length: 346
  • theoretical pI: 7.16655
  • theoretical MW: 39016.1
  • GRAVY: -0.00635838

⊟Function[edit | edit source]

  • TIGRFAM:
    gliding motility-associated protein GldE (TIGR03520; HMM-score: 207.6)
    and 1 more
    Hypothetical proteins Conserved TIGR00304 family protein (TIGR00304; HMM-score: 7.6)
  • TheSEED  :
    • Hemolysins and related proteins containing CBS domains
  • PFAM:
    no clan defined DUF21; Domain of unknown function DUF21 (PF01595; HMM-score: 157.2)
    and 2 more
    CBS; CBS domain (PF00571; HMM-score: 30.5)
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 11.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.68
    • Cellwall Score: 0.17
    • Extracellular Score: 0.16
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide SP(Sec/SPI) length 23 aa
    • SP(Sec/SPI): 0.647135
    • TAT(Tat/SPI): 0.007514
    • LIPO(Sec/SPII): 0.16086
    • Cleavage Site: CS pos: 23-24. ALT-AA. Pr: 0.2311
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGIVKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVIPKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKEEFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVYETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKENQITNYSAKPLFVNEHNKAEWVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQIQFQQRKNRNVSI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [4] [3]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  4. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SAOUHSC_00854&oldid=97220"