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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0954 [new locus tag: SACOL_RS04890 ]
  • pan locus tag?: SAUPAN003052000
  • symbol: mnhB
  • pan gene symbol?: mnhB1
  • synonym:
  • product: monovalent cation/H+ antiporter subunit B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0954 [new locus tag: SACOL_RS04890 ]
  • symbol: mnhB
  • product: monovalent cation/H+ antiporter subunit B
  • replicon: chromosome
  • strand: -
  • coordinates: 954742..955170
  • length: 429
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAATAGACAACAAAATGATCTAATTTTGCAATTTGCTGCAGTTATTATCTTTTTCATG
    GTAATGGTCTTTGGTTTTTCACTTTTCTTAGCAGGACATTATACACCTGGTGGCGGTTTC
    GTCGGCGGATTATTATTCGCAAGTTCACTTGTTATCATTACGATTGCATTTGATATAGAA
    ACAATGCGTAAAATTTTCCCATTAGATTTTAAAATCTTGATAGGTATCGGATTAGTTTTT
    TGTATTGCGACGCCTATCGCAAGTTGGTTTTTAGGCAAAAATTTCTTTACGCATGTCACA
    TTTGATATACCGTTATTTATATTAGAACCTGTTCATATGACAACAGCAGTATTCTTTGAT
    TTCGGTGTTTTATGTGCAGTAGTTGGTACTGTCATGACAATTATTATTTCGATTGGAGAG
    AACGAATAG
    60
    120
    180
    240
    300
    360
    420
    429

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0954 [new locus tag: SACOL_RS04890 ]
  • symbol: MnhB
  • description: monovalent cation/H+ antiporter subunit B
  • length: 142
  • theoretical pI: 4.88938
  • theoretical MW: 15681.7
  • GRAVY: 1.19155

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Cations and iron carrying compounds monovalent cation:proton antiporter (TIGR00943; HMM-score: 160.7)
  • TheSEED  :
    • Na(+) H(+) antiporter subunit B (TC 2.A.63.1.3)
    Membrane Transport Uni- Sym- and Antiporters Sodium Hydrogen Antiporter  Na(+) H(+) antiporter subunit B (TC 2.A.63.1.3)
  • PFAM:
    no clan defined MnhB; Domain related to MnhB subunit of Na+/H+ antiporter (PF04039; HMM-score: 100.6)
    and 1 more
    NfeD-like (CL0252) NfeD; NfeD-like C-terminal, partner-binding (PF01957; HMM-score: 7.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004243
    • TAT(Tat/SPI): 0.000461
    • LIPO(Sec/SPII): 0.00489
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGLLFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFDIPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Integral membrane [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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